Temperature-Controlled Structure and Kinetics of Ripple Phases in One- and Two-Component Supported Lipid Bilayers

Kaasgaard, Thomas; Leidy, Chad; Crowe, John H.; Mouritsen, Ole G.; Jørgensen, Kent

doi:10.1016/s0006-3495(03)74479-8

Cited by 128 publications

(149 citation statements)

References 42 publications

Supporting

Mentioning

130

Contrasting

Order By: Relevance

“…For example, ripple domains (in the nanometer range) were described for this particular mixture at temperatures similar to those studied here (50). The equilibrium between lipid phases creates special stress in the vesicle structure.…”

Section: Discussionsupporting

confidence: 61%

Visualization and analysis of apolipoprotein A-I interaction with binary phospholipid bilayers

Tricerri

Toledo

Sánchez

et al. 2005

Journal of Lipid Research

View full text Add to dashboard Cite

Apolipoprotein A-I (apoA-I) interaction with specific cell lipid domains was suggested to trigger cholesterol and phospholipid efflux. We analyzed here apoA-I interaction with dimyristoylphosphatidylcholine/distearoylphosphatidylcholine (DMPC/DSPC) bilayers at a temperature showing phase coexistence. Solid and liquid-crystalline domains were visualized by two-photon fluorescence microscopy on giant unilamellar vesicles (GUVs) labeled with 6-dodecanoyl-2-dimethylamino-naphthalene (Laurdan). A decrease of vesicle size was detected as long as they were incubated with lipid-free apoA-I, together with a shape deformation and a relative enrichment in DSPC. Selective lipid removal mediated by apoA-I from different domains was followed in real time by changes in the Laurdan generalized polarization. The data show a selective interaction of apoA-I with liquid-crystalline domains, from which it removes lipids, at a molar ratio similar to the domain compositions. Next, apoA-I was incubated with DMPC/DSPC small unilamellar vesicles, and products were isolated and quantified. Protein solubilized both lipids but formed complexes relatively enriched in the liquid component. We also show changes in the GUV morphology when cooling down. Our results suggest that the most efficient reaction between apoA-I and DMPC/DSPC occurs in particular bilayer conditions, probably when small fluid domains are nucleated within a continuous gel phase and interfacial packing defects are maximal.

show abstract

Section: Discussionsupporting

confidence: 61%

Visualization and analysis of apolipoprotein A-I interaction with binary phospholipid bilayers

Tricerri

Toledo

Sánchez

et al. 2005

Journal of Lipid Research

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

“…The P β′ ripple phase in supported double bilayers only existed in a small temperature range (31). Decreasing temperature to below the pretransition temperature resulted in the dissipation of the P β′ phase (31). However, the patch domains in GUVs are stable with temperature decrease and do not change into the stripe domains below the pretransition temperature of DPPC ( Figure 6).…”

Section: Discussionmentioning

confidence: 99%

Coexisting Stripe- and Patch-Shaped Domains in Giant Unilamellar Vesicles

Cheng

2006

Biochemistry

View full text Add to dashboard Cite

We report a new type of gel-liquid phase segregation in giant unilamellar vesicles (GUVs) of mixed lipids. Coexisting patch and stripe shaped gel domains in GUV bilayers composed of DOPC/DPPC or DLPC/DPPC are observed by confocal fluorescence microscopy. The lipids in stripe domains are shown to be tilted according to the DiIC18 fluorescence intensity dependence on the excitation polarization. The patch domains are found to be mainly composed of d62-DPPC according to the coherent anti-Stokes Raman scattering (CARS) images of DOPC/d62-DPPC bilayers. When cooling GUVs from above the miscibility temperature, the patch domains start to appear between the chain melting and the pretransition temperature of DPPC. In GUVs containing high molar percentage of DPPC, the stripe domains form below the pretransition temperature. Our observations suggest that the patch and stripe domains are in the P β′ and L β′ gel phases, respectively. According to the thermoelastic properties of GUVs described by Needham and Evans (Biochemistry,27,[8261][8262][8263][8264][8265][8266][8267][8268][8269], the P β′ and L β′ phases are formed at relatively low and high membrane tensions respectively. GUVs with high DPPC percentage have high membrane surface tension and thus mainly exhibit L β′ domains, while GUVs with low DPPC percentage have low membrane surface tension and form P β′ domains accordingly. Adding negatively charged lipid to the lipid mixtures or applying an osmotic pressure to GUVs using sucrose solutions releases the surface tension and leads to the disappearance of L β′ gel phase. The relationship between the observed domains in free-standing GUV bilayers in and those in supported bilayers is discussed.Phase segregation plays important roles in membrane organization and intracellular trafficking (1-5). Phase behavior of lipid mixtures has been extensively studied using different welldefined model membrane systems. GUVs which mimic the plasma membrane with controlled lipid compositions and similar size as cells have been widely used to study the mechanisms of membrane phase segregation, fusion, and fission (6-10). In GUVs composed of a saturated lipid, an unsaturated lipid, and cholesterol, the observation of coexisting ordered and disordered liquid phases below miscibility transition temperature has provided supports for the existence of lipid rafts in cell membranes (11). Despite these advances, the lipid phase behavior in GUVs is far away from being well understood, even for binary lipid mixtures. Patch and stripe shaped domains in GUVs composed of DPPC and another phospholipid have been reported in different papers (7,8,12,13). However, the composition and lipid organization properties of these domains have not been well characterized. The relations between these two types of domains and the different gel phases (P β′ and L β′ ) observed in pure DPPC bilayers are elusive. In addition, the forces that govern the formation of these two types of domains are poorly investigated.To whom correspondence should be addressed....

show abstract

“…Both modes have been simulated using coarsegrained molecular dynamics [28][29][30]. The oscillatory mode corresponds to ripple phases that have been detected experimentally [31]. Evidently, these emerge when F(d) possesses a local maximum at d 0 .…”

Section: (B) Examplementioning

confidence: 87%