Temperature-controlled microintaglio printing for high-resolution micropatterning of RNA molecules

Kobayashi, Ryo; Biyani, Manish; Ueno, S.; Kumal, Subhashini Raj; Kuramochi, Hiromi; Ichikı, Takanori

doi:10.1016/j.bios.2014.07.050

Cited by 4 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[103] A microintaglio printing method for RNA arraying has been optimized, by exploiting the complementarity of the DNA probes with the RNA sequences. [104] Finally, more complex structures of RNA, in particular RNA aptamers, can be deposited on solid supports by inkjet printing, without any loss of functionality. [105] However, the lower chemical stability of RNA oligonucleotides with respect to DNA, and the possibility to use equivalent complementary DNA (cDNA) sequences has limited the use of RNA in printing.…”

Section: Nucleic Acidsmentioning

confidence: 99%

Artificial Biosystems by Printing Biology

Arrabito

Ferrara

Bonasera

et al. 2020

Small

View full text Add to dashboard Cite

The continuous progress of printing technologies over the past 20 years has fueled the development of a wide plethora of applications in materials sciences, flexible electronics and biotechnologies. More recently, printing methodologies have started up to explore the world of Artificial Biology, offering new paradigms in the direct assembly of Artificial Biosystems (small condensates, compartments, networks, tissues and organs) by mimicking the result of the evolution of living systems and also by redesigning natural biological systems, taking inspiration from them. This recent progress is reported in terms of a new field here defined as Printing Biology, resulting from the intersection between the field of printing and the bottom up Synthetic Biology. Printing Biology explores new approaches for the reconfigurable assembly of designed lifelike or life-inspired structures. This review presents this emerging field, highlighting its main features, i.e. printing methodologies (from 2D to 3D), molecular ink properties, deposition mechanisms, and finally the applications and future challenges. Printing Biology is expected to show a growing impact on the development of biotechnology and lifeinspired fabrication. Printing Biology employs different technologies dispensing molecular inks with tunable composition (molecules, polymers, biomolecules) and drop sizes (from nano-up to macroscale) onto solids or into liquids to develop lifelike or life-inspired artificial biosystems (from small condensates, to compartments up to networks, tissues and organs). This work reviews the extraordinary potential of this emerging research field.

show abstract

Section: Nucleic Acidsmentioning

confidence: 99%

Artificial Biosystems by Printing Biology

Arrabito

Ferrara

Bonasera

et al. 2020

Small

View full text Add to dashboard Cite

show abstract

“…This approach was further improved using poly(dimethylsiloxane) (PDMS) for the creation of a soft lithography-based microintaglio printing method to generate high-density protein arrays ( 78 ). A year later, temperature controlled microintaglio printing (TC-μTP) was developed and used to generate RNA microarrays using DNA-immobilized magnetic beads ( 79 ). The second and very similar method that aimed to improve the density of protein arrays uses on-chip micro compartmentalization of protein synthesis in arrayed micrometer scale chambers from confined DNA template molecules ( 80 ).…”

Section: Screening Of Proteins On 2d Microarraysmentioning

confidence: 99%

High-throughput screening of biomolecules using cell-free gene expression systems

Contreras-Llano

Tan

2018

Synthetic Biology

View full text Add to dashboard Cite

The incorporation of cell-free transcription and translation systems into high-throughput screening applications enables the in situ and on-demand expression of peptides and proteins. Coupled with modern microfluidic technology, the cell-free methods allow the screening, directed evolution and selection of desired biomolecules in minimal volumes within a short timescale. Cell-free high-throughput screening applications are classified broadly into in vitro display and on-chip technologies. In this review, we outline the development of cell-free high-throughput screening methods. We further discuss operating principles and representative applications of each screening method. The cell-free high-throughput screening methods may be advanced by the future development of new cell-free systems, miniaturization approaches, and automation technologies.

show abstract

“…To accomplish high-contrast µIP, we have recently introduced an advanced form of µIP by controlling the adsorption and/or printing reaction of the presynthesized ink molecules via a thermal trigger (Figure 5) [30]. This is based on a controllable hybridization reaction between a presynthesized messenger RNA and a substrate-immobilized complementary DNA probe by switching the ability of the messenger RNA to fold into a secondary structure via the temperature during µIP processing.…”

Section: Microintaglio Printing-based Biomolecular Microarraysmentioning

confidence: 99%

“…Therefore, temperature switching before and after the press-sealing step in the µIP process can prevent the undesired printing of presynthesized messenger RNA molecules in the non-array region (Figure 5a). Using this method, we reported the fabrication of transcribed RNA microarrays with a diameter of 1.5 µm and a density of 40,000 spots per mm 2 with high contrast (Figure 5b,c) [30]. Most importantly, we also demonstrated that the uniformity of patterned signals over a range of microarray feature sizes spanning one order of magnitude is not affected, and thus, this method can be used for the miniaturization of arrays to obtain high-density nanoarrays.…”

Section: Microintaglio Printing-based Biomolecular Microarraysmentioning

confidence: 99%

Microintaglio Printing for Soft Lithography-Based in Situ Microarrays

Biyani

Ichikı

2015

Microarrays

Self Cite

View full text Add to dashboard Cite

Advances in lithographic approaches to fabricating bio-microarrays have been extensively explored over the last two decades. However, the need for pattern flexibility, a high density, a high resolution, affordability and on-demand fabrication is promoting the development of unconventional routes for microarray fabrication. This review highlights the development and uses of a new molecular lithography approach, called “microintaglio printing technology”, for large-scale bio-microarray fabrication using a microreactor array (µRA)-based chip consisting of uniformly-arranged, femtoliter-size µRA molds. In this method, a single-molecule-amplified DNA microarray pattern is self-assembled onto a µRA mold and subsequently converted into a messenger RNA or protein microarray pattern by simultaneously producing and transferring (immobilizing) a messenger RNA or a protein from a µRA mold to a glass surface. Microintaglio printing allows the self-assembly and patterning of in situ-synthesized biomolecules into high-density (kilo-giga-density), ordered arrays on a chip surface with µm-order precision. This holistic aim, which is difficult to achieve using conventional printing and microarray approaches, is expected to revolutionize and reshape proteomics. This review is not written comprehensively, but rather substantively, highlighting the versatility of microintaglio printing for developing a prerequisite platform for microarray technology for the postgenomic era.

show abstract

Temperature-controlled microintaglio printing for high-resolution micropatterning of RNA molecules

Cited by 4 publications

References 33 publications

Artificial Biosystems by Printing Biology

Artificial Biosystems by Printing Biology

High-throughput screening of biomolecules using cell-free gene expression systems

Microintaglio Printing for Soft Lithography-Based in Situ Microarrays

Contact Info

Product

Resources

About