Temperature-control-induced masculinization in tiger puffer Takifugu rubripes

He, Zhou; Zhuang, Zixin; Zhang, Rui; Xu, Qi-Zheng; Liang, Yuting; Jiang, Zhiqiang; Li, Xia; Ma, Tianyu; Li, Yajuan

doi:10.1007/s00343-019-7382-z

Cited by 10 publications

(9 citation statements)

References 20 publications

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“…The fish samples used in this study was produced by the low-temperature treatment induced masculinization of fugu in Zhou et al [21]. Briefly, an orthogonal test L 9 (3 4 ) design was chosen with three factors at three levels: treatment starting times (days post-hatch, dph) of 20, 50, 80 dph; treatment temperatures of 13 °C, 15 °C and 17 °C; treatment duration of 30, 45, 60 days.…”

Section: Methodsmentioning

confidence: 99%

Changes in DNA methylation during epigenetic-associated sex reversal under low temperature in Takifugu rubripes

Zhuang

Sun

et al. 2019

PLoS ONE

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DNA methylation has frequently been implicated in sex determination and differentiation in teleost species. In order to detect the DNA methylation patterns established during sexual differentiation in tiger pufferfish T . rubripes , we performed comprehensive whole genome methylation sequencing and analyses of the gonads of male, female, and pseudo male. We obtained a total of 33.12, 32.44, and 31.60 Gb clean data for male, female, and pseudo male, with a sequencing depth of 66.44×, 60.47× and 54.86×, respectively. The methylation level of cytosine (C) residues in the genomic DNA from gonads was 11.016%, 10.428%, and 11.083% in male, female, and pseudo male, respectively. More than 65% of C methylation was at CpG sites, and less than 1% was at CHG and CHH sites. In each regulatory element, there were low methylation levels on both sides of the transcription start site, and higher methylation levels in exons, introns, and downstream of genes. The highest mCpG was on chromosome 8 and the lowest mCpG was on chromosome 5. Comparisons of whole-genome DNA methylation between pairs of samples revealed that there were 3,173 differentially methylated regions (DMRs) between female and male, and 3,037 DMRs between male and pseudo male, corresponding to 0.232% and 0.223% of the length of the genome, respectively. There were only 1,635 DMRs between female and pseudo male, representing 0.127% of the length of the genome. A number of differentially methylated genes (DMGs) related to sex determination and differentiation were selected, such as amhr2 and pfcyp19a . After Bisulfite Sequencing PCR (BSP) verification, amhr2 was exhibited low methylation level in normal males and pseudo male, and high methylation level in normal females but pfcyp19a showed low methylation level in normal females and high methylation level in normal males and pseudo males. These results provide information about the molecular epigenetic mechanisms of DNA methylation during low-temperature induced masculinization of tiger pufferfish, and increase our understanding of the mechanisms of sex determination and differentiation in this important aquaculture fish species.

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Section: Methodsmentioning

confidence: 99%

Changes in DNA methylation during epigenetic-associated sex reversal under low temperature in Takifugu rubripes

Zhuang

Sun

et al. 2019

PLoS ONE

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“…The types of potential temperature effects on shifts in population sex ratio: Decrease (a) or increase (b) in proportion of males with increasing temperature; decrease or increase in proportion of males at extreme temperatures (c). Most observations so far show male biases at high temperature (emphasized in dark grey, examples mentioned in Introduction), but other sex ratio biases have been reported (emphasized in light grey), for example: A1, tiger puffer Takifugu rubripes (Zhou et al ., 2019); A2, channel catfish Ictalurus punctatus (Patiño et al ., 1996); B1, tidewater silverside Menidia peninsulae (Middaugh & Hemmer, 1987); C2, southern flounder Paralichthys lethostigma (Luckenbach et al ., 2003)…”

Section: Types Of Temperature Effects On Sex Determinationmentioning

confidence: 99%

Effects of global warming on sex ratios in fishes

Geffroy

Wedekind

2020

Journal of Fish Biology

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In fishes, sex is determined by genetics, the environment or an interaction of both. Temperature is among the most important environmental factors that can affect sex determination. As a consequence, changes in temperature at critical developmental stages can induce biases in primary sex ratios in some species. However, early sex ratios can also be biased by sex‐specific tolerances to environmental stresses that may, in some cases, be amplified by changes in water temperature. Sex‐specific reactions to environmental stress have been observed at early larval stages before gonad formation starts. It is therefore necessary to distinguish between temperature effects on sex determination, generally acting through the stress axis or epigenetic mechanisms, and temperature effects on sex‐specific mortality. Both are likely to affect sex ratios and hence population dynamics. Moreover, in cases where temperature effects on sex determination lead to genotype–phenotype mismatches, long‐term effects on population dynamics are possible, for example temperature‐induced masculinization potentially leading to the loss of Y chromosomes or feminization to male‐biased operational sex ratios in future generations. To date, most studies under controlled conditions conclude that if temperature affects sex ratios, elevated temperatures mostly lead to a male bias. The few studies that have been performed on wild populations seem to confirm this general trend. Recent findings suggest that transgenerational plasticity could mitigate the effects of warming on sex ratios in some populations.

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“…The types of potential temperature effects on shifts in population sex ratio: Decrease (A) or increase (B) in proportion of males with increasing temperature; decrease or increase in proportion of males at extreme temperatures (C). Most observations so far show male biases at high temperature (emphasized in dark grey, examples mentioned in Introduction), but other sex ratio biases have been reported (emphasized in light grey), for example A1: tiger puffer Takifugu rubripes (Zhou et al, 2019), A2: channel catfish Ictalurus punctatus (Patiño et al 1996), B1 : tidewater silverside Menidia peninsulae (Middaugh & Hemmer, 1987), C2: southern flounder Paralichthys lethostigma (Luckenbach et al, 2003). .…”

Section: Discussionmentioning

confidence: 99%

Effects of global warming on sex ratios in fishes

2021

Journal of Fish Biology

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Temperature-control-induced masculinization in tiger puffer Takifugu rubripes

Cited by 10 publications

References 20 publications

Changes in DNA methylation during epigenetic-associated sex reversal under low temperature in Takifugu rubripes

Changes in DNA methylation during epigenetic-associated sex reversal under low temperature in Takifugu rubripes

Effects of global warming on sex ratios in fishes

Effects of global warming on sex ratios in fishes

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