Temperature “breaks” in Arrhenius plots: A thermodynamic consequence of a phase change

Kumamoto, Junh; Raison, John K.; Lyons, Joseph

doi:10.1016/0022-5193(71)90120-2

Cited by 179 publications

(59 citation statements)

References 9 publications

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“…1 deviates from a straight line at ~24 °C and reveals values of activation energy similar to those measured for cleavage of the GPI-anchored protein, 5′-nucleotidase, from the surface of porcine lymphocyte PM [59]. A biphasic Arrhenius plot is common for many membrane-bound enzymes and may result from a lipid phase transition from a gel to a fluid liquid crystalline phase, from lipid phase separation within the membrane [60,25], or from other factors such as a change in activation entropy or changes in membrane components other than lipids [59].…”

Section: Dynamic Monitoring Of Membrane Traffickingmentioning

confidence: 54%

Regulation of membrane trafficking and subcellular organization of endocytic compartments revealed with FM1-43 in resting and activated human T cells

Fomina

Deerinck

Ellisman

et al. 2003

Experimental Cell Research

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FM1-43, a fluorescent styryl dye that penetrates into and stains membranes, was used to investigate kinetics of constitutive endocytosis and to visualize the fate of endocytic organelles in resting and activated human T lymphocytes. The rate of dye accumulation was strongly temperature dependent and ~10-fold higher in activated than in resting T cells. Elevation of cytosolic free Ca 2+ concentration with thapsigargin or ionomycin further accelerated the rate of FM1-43 accumulation associated with cytosolic actin polymerization. Direct modulation of actin polymerization affected membrane trafficking. Actin condensation beneath the plasma membrane with calyculin A abolished FM1-43 internalization, whereas actin depolymerization with cytochalasin D had no effect. Photoconversion of DAB by FM1-43 revealed altered endocytic compartment targeting associated with T cell activation. Internalized cargo was carried to lysosome-like compartments in resting T cells and to multivesicular bodies (MVB) in activated T cells. Externalization of exosomes from MVB occurred commonly in activated but not in resting T cells. T cell exosomes contained raft-associated CD3 proteins, GM1 glycosphingolipids, and phosphatidylserine at the outer membrane leaflet. The present study demonstrates the utility of FM1-43 as a marker of membrane trafficking in T cells and reveals possible mechanisms of its modulation during T cell activation.

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Section: Dynamic Monitoring Of Membrane Traffickingmentioning

confidence: 54%

Regulation of membrane trafficking and subcellular organization of endocytic compartments revealed with FM1-43 in resting and activated human T cells

Fomina

Deerinck

Ellisman

et al. 2003

Experimental Cell Research

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“…1) suggests another possible role for membranes. Many membrane-related processes are known to change their rates at break-point temperatures (22)(23)(24)(25) (26); variation of the cel phase-transition temperature with different fatty acid supplements could account for the supplement effects (27). Alternatively, the temperaturecompensation mechanism in cel-, but not in cel+, could be sensitive to some phase transition that would be present in both strains near 22TC.…”

Section: Discussionmentioning

confidence: 99%

Circadian rhythms in Neurospora crassa: a mutation affecting temperature compensation.

Mattern¹,

Forman²,

Brody³

1982

Proc. Natl. Acad. Sci. U.S.A.

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The circadian rhythm of conidiation (spore formation) in Neurospora crassa is known to be temperature compensated, that is, the period is only slightly affected by the incubation temperature. Thus, the Q10 (the relative rate enhancement corresponding to a 10C rise in temperature) of the rhythm of the bd cap strain from 14 to 300C was 1.1, whereas the Q10 of the uncompensated growth rate in the same interval was 2.4. A mutation at the cel locus resulted in loss of the temperature-compensation property in cultures grown below 22TC. The Q10 of the rhythm below 220C was 2.2, and periods of about 40 hr were observed. In contrast, the Q10 of the rhythm above 220C was 1.1, with circadian periods of 18-21 hr. Thus, cel displayed a threshold temperature or "break point" for the temperature compensation of its rhythm. Supplementation of cel strains, which require fatty acids, with unsaturated or short-chain fatty acids raised the threshold temperature to 260C, whereas supplementation with long-chain saturated fatty acids lowered it to 18C. These data suggest a role for fatty acids, as lipid components or as cellular metabolites, in the mechanism of temperature compensation.Circadian rhythms are the most widely studied of the endogenous biological oscillations. Three characteristics are ordinarily used to identify a rhythm as circadian: its period (about 24 hr when free-running); its sensitivity to light (reflecting the ability to adjust its phase to the phase of the environment); and the relative insensitivity of its period to the growth temperature (1). This last characteristic is called temperature compensation (2). Without such a safeguard, daily biological timekeeping would be highly unreliable, running ahead oflocal time in warm situations (summer, noon, thermal springs) and behind in cool ones (winter, midnight, mountain streams). Examples of uncompensated daily rhythms are rare, and are usually confined to tropical species, which live where ambient temperatures are fairly constant (3).Temperature compensation is often quantified in terms ofthe temperature coefficient Q1o, the relative rate enhancement of a process corresponding to a 100C rise in temperature. Ordinary biological growth rates and reactions have Q10s of 2-3 (2). The

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“…Our reasoning rests on the circumstance that the activities of membrane-bound enzymes, including the protein-synthesizing machinery on membrane-bound ribosomes, also decrease with temperature, observations reasonably related to the thermotropic transitions of the membrane lipids (e.g . 42,26,57,63) . We are expanding the above experiments by gel electrophoretic analyses of the RNA synthesized in the nuclei and transported to the cytoplasm in Tetrahvmena adapted to low temperatures .…”

Section: Discussion Nuclear Membrane Transitionsmentioning

confidence: 99%

REVERSIBLE, THERMOTROPIC ALTERATION OF NUCLEAR MEMBRANE STRUCTURE AND NUCLEOCYTOPLASMIC RNA TRANSPORT IN TETRAHYMENA

Wunderlich

Batz

Speth

et al. 1974

The Journal of Cell Biology

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We examine the effect of cooling upon the freeze-etch ultrastructure of nuclear membranes, as well as upon nucleocytoplasmic RNA transport in the unicellular eukaryote Tetrahymena pyriformis . Chilling produces smooth, particle-free areas on both faces of the two freeze-fractured macronuclear membranes . Upon return to optimum growth temperature the membrane-associated particles revert to their normal uniform distribution and the smooth areas disappear . Chilling lowers the incorporation of [ 14 C]uridine into whole cells and their cytoplasmic RNA . Cooling from the optimum growth temperature of 28°to 18°C (or above) decreases [ 14 C]uridine incorporation into cells more than into their cytoplasmic RNA ; chilling to below 18°C but above 10°C causes the reverse .[ 14 C]Uridine incorporation into whole cells and their cytoplasmic RNA reflects overall RNA synthesis and nucleocytoplasmic RNA transport, respectively . RNA transport decreases strongly between 20°and 16°C, which is also the temperature range where morphologically detectable nuclear membrane transitions occur . This suggests that the nuclear envelope limits the rate of nucleocytoplasmic RNA transport at low temperatures . We hypothesize that a thermotropic lipid phase transition switches nuclear pore complexes from an "open" to a "closed" state with respect to nucleocytoplasmic RNA transport .

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Temperature “breaks” in Arrhenius plots: A thermodynamic consequence of a phase change

Cited by 179 publications

References 9 publications

Regulation of membrane trafficking and subcellular organization of endocytic compartments revealed with FM1-43 in resting and activated human T cells

Regulation of membrane trafficking and subcellular organization of endocytic compartments revealed with FM1-43 in resting and activated human T cells

Circadian rhythms in Neurospora crassa: a mutation affecting temperature compensation.

REVERSIBLE, THERMOTROPIC ALTERATION OF NUCLEAR MEMBRANE STRUCTURE AND NUCLEOCYTOPLASMIC RNA TRANSPORT IN TETRAHYMENA

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