Temperature and water loss affect ADH activity and gene expression in grape berry during postharvest dehydration

Cirilli, Marco; Bellincontro, Andrea; Santis, Diana De; Botondi, R.; Colao, Maria Chiara; Muleo, Rosario; Mencarelli, Fabio

doi:10.1016/j.foodchem.2011.11.020

Cited by 62 publications

(51 citation statements)

References 36 publications

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“…However, ADH3 showed higher scores than ADH2 and more relationships with other metabolites such as (E)-2-hexenal (0.67), 3-ethylbenzaldehyde (0.84), 2-ethylbenzaldehyde (0.74), terpeniol (0.73) and 2,6-diisocyanatotoluene (0.82). It has been shown that grape ADH1 gene expression was detected in the first phase of fruit development, while ADH2 has been described as a berry ripening-related isogene, with data suggesting that transcriptional regulation of these genes and ADH enzyme activity could partially be related to the ethylene signaling pathway (Cirilli et al, 2012). …”

Section: Resultsmentioning

confidence: 99%

Gene-Metabolite Networks of Volatile Metabolism in Airen and Tempranillo Grape Cultivars Revealed a Distinct Mechanism of Aroma Bouquet Production

Rambla

Trapero-Mozos

Diretto³

et al. 2016

Front. Plant Sci.

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Volatile compounds are the major determinants of aroma and flavor in both grapes and wine. In this study, we investigated the emission of volatile and non-volatile compounds during berry maturation in two grape varieties (Airén and Tempranillo) throughout 2010 and 2011. HS-SPME coupled to gas chromatography and mass spectrometry was applied for the identification and relative quantitation of these compounds. Principal component analysis was performed to search for variability between the two cultivars and evolution during 10 developmental stages. Results showed that there are distinct differences in volatile compounds between cultivars throughout fruit development. Early stages were characterized in both cultivars by higher levels of some apocarotenoids such as β-cyclocitral or β-ionone, terpenoids (E)-linalool oxide and (Z)-linalool oxide and several furans, while the final stages were characterized by the highest amounts of ethanol, benzenoid phenylacetaldehyde and 2-phenylethanol, branched-amino acid-derived 3-methylbutanol and 2-methylbutanol, and a large number of lipid derivatives. Additionally, we measured the levels of the different classes of volatile precursors by using liquid chromatography coupled to high resolution mass spectrometry. In both varieties, higher levels of carotenoid compounds were detected in the earlier stages, zeaxanthin and α-carotene were only detected in Airén while neoxanthin was found only in Tempranillo; more variable trends were observed in the case of the other volatile precursors. Furthermore, we monitored the expression of homolog genes of a set of transcripts potentially involved in the biosynthesis of these metabolites, such as some glycosyl hydrolases family 1, lipoxygenases, alcohol dehydrogenases hydroperoxide lyases, O-methyltransferases and carotenoid cleavage dioxygenases during the defined developmental stages. Finally, based on Pearson correlation analyses, we explored the metabolite-metabolite fluctuations within VOCs/precursors during the berry development; as well as tentatively linking the formation of some metabolites detected to the expression of some of these genes. Our data showed that the two varieties displayed a very different pattern of relationships regarding the precursor/volatile metabolite-metabolite fluctuations, being the lipid and the carotenoid metabolism the most distinctive between the two varieties. Correlation analysis showed a higher degree of overall correlation in precursor/volatile metabolite-metabolite levels in Airén, confirming the enriched aroma bouquet characteristic of the white varieties.

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Section: Resultsmentioning

confidence: 99%

Gene-Metabolite Networks of Volatile Metabolism in Airen and Tempranillo Grape Cultivars Revealed a Distinct Mechanism of Aroma Bouquet Production

Rambla

Trapero-Mozos

Diretto³

et al. 2016

Front. Plant Sci.

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“…Environmental stresses such as hypoxia (Johnson et al, 1994), drought (Senthil-Kumar et al, 2010), and abscisic acid phytohormone (Zhang et al, 1997) induce a high expression of ADH1 . ADH gene expression, enzyme activity, and related metabolites were analyzed under different temperatures, and the results revealed that the levels of acetaldehyde, ethanol, acetic acid, and ethyl acetate fluctuated at 10°, 20°, and 30°C (Cirilli et al, 2012), suggesting temperature affecting ADH activity and the accumulation of related metabolites. Formate levels were reduced, acetate levels were elevated, and extracellular glycerol accumulated in adh1 mutant of Chlamydomonas reinhardtii under anoxic conditions (Magneschi et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Metabolite Profiling of adh1 Mutant Response to Cold Stress in Arabidopsis

et al. 2017

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As a result of global warming, vegetation suffers from repeated freeze-thaw cycles caused by more frequent short-term low temperatures induced by hail, snow, or night frost. Therefore, short-term freezing stress of plants should be investigated particularly in light of the current climatic conditions. Alcohol dehydrogenase (ADH) plays a central role in the metabolism of alcohols and aldehydes and it is a key enzyme in anaerobic fermentation. ADH1 responds to plant growth and environmental stress; however, the function of ADH1 in the response to short-term freezing stress remains unknown. Using real-time quantitative fluorescence PCR, the expression level of ADH1 was analyzed at low temperature (4°C). The lethal temperature was calculated based on the electrolyte leakage tests for both ADH1 deletion mutants (adh1) and wild type (WT) plants. To further investigate the relationship between ADH1 and cold tolerance in plants, low-Mr polar metabolite analyses of Arabidopsis adh1 and WT were performed at cold temperatures using gas chromatography-mass spectrometry. This investigation focused on freezing treatments (cold acclimation group: −6°C for 2 h with prior 4°C for 7 d, cold shock group: −6°C for 2 h without cold acclimation) and recovery (23°C for 24 h) with respect to seedling growth at optimum temperature. The experimental results revealed a significant increase in ADH1 expression during low temperature treatment (4°C) and at a higher lethal temperature in adh1 compared to that in the WT. Retention time indices and specific mass fragments were used to monitor 263 variables and annotate 78 identified metabolites. From these analyses, differences in the degree of metabolite accumulation between adh1 and WT were detected, including soluble sugars (e.g., sucrose) and amino acids (e.g., asparagine). In addition, the correlation-based network analysis highlighted some metabolites, e.g., melibiose, fumaric acid, succinic acid, glycolic acid, and xylose, which enhanced connectedness in adh1 network under cold chock. When considered collectively, the results showed that adh1 possessed a metabolic response to freezing stress and ADH1 played an important role in the cold stress response of a plant. These results expands our understanding of the short-term freeze response of ADH1 in plants.

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“…The relative expression of CsADH that the physiological response of grapes preceded the changes in gene expression (Cirilli et al, 2012). The relative expression of CsADH that the physiological response of grapes preceded the changes in gene expression (Cirilli et al, 2012).…”

Section: Expression and Regulation Of The Adh Gene During The Posthmentioning

confidence: 99%

“…Much work has shown that ADH genes can respond to dehydration (Cirilli et al, 2012), low temperature (Strommer, 2011), and abscisic acid (Lu, Paul, Mccarty, & Ferl, 2018 ) and play essential roles in fruit ripening (Hao, Cao, Jin, Tang, & Qi, 2016), seedling development (Díaz, Fernández, & Martínez, 2016), and pollen formation (Freeling, 1978). Much work has shown that ADH genes can respond to dehydration (Cirilli et al, 2012), low temperature (Strommer, 2011), and abscisic acid (Lu, Paul, Mccarty, & Ferl, 2018 ) and play essential roles in fruit ripening (Hao, Cao, Jin, Tang, & Qi, 2016), seedling development (Díaz, Fernández, & Martínez, 2016), and pollen formation (Freeling, 1978).…”

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confidence: 99%

Dynamics of ADH and related genes responsible for the transformation of C₆‐aldehydes to C₆‐alcohols during the postharvest process of oolong tea

Zhou

Yao

et al. 2019

Food Science & Nutrition

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Aroma is an important index of tea quality. The volatile C6‐compounds formed from linoleic and linolenic acids in tea leaf lipids are essential components of tea. C6‐compounds are formed and transformed during the postharvest process of tea leaves. However, the metabolic flux of these C6‐compounds, the activities of related enzymes, and the transcription of related genes during the postharvest process of oolong tea remain unclear. In this study, the chemical profiles of C6‐aldehydes and C6‐alcohols, the pattern of ADH enzyme activity, and the level of CsADH gene expression during the postharvest process of oolong tea were investigated. We found that the turnover process had a positive effect on the accumulation of C6‐alcohols and simultaneously induced ADH activity, especially during the withering stage. The expression of CsADH peaked during the turnover stage. The relative expression level of CSA019598 typically increased during the postharvest process. Correlation analysis demonstrated that CSA019598 expression increased as ADH activity increased. This finding suggests that CSA019598 may play a prominent role in regulating ADH. These results advance our understanding of C6‐compound formation during the postharvest process of oolong tea. We aim to evaluate how green leaf volatiles affect the enzymatic formation and genetic transcription of aromatic compounds in oolong tea in future studies.

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Temperature and water loss affect ADH activity and gene expression in grape berry during postharvest dehydration

Cited by 62 publications

References 36 publications

Gene-Metabolite Networks of Volatile Metabolism in Airen and Tempranillo Grape Cultivars Revealed a Distinct Mechanism of Aroma Bouquet Production

Gene-Metabolite Networks of Volatile Metabolism in Airen and Tempranillo Grape Cultivars Revealed a Distinct Mechanism of Aroma Bouquet Production

Metabolite Profiling of adh1 Mutant Response to Cold Stress in Arabidopsis

Dynamics of ADH and related genes responsible for the transformation of C₆‐aldehydes to C₆‐alcohols during the postharvest process of oolong tea

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Temperature and water loss affect ADH activity and gene expression in grape berry during postharvest dehydration

Cited by 62 publications

References 36 publications

Gene-Metabolite Networks of Volatile Metabolism in Airen and Tempranillo Grape Cultivars Revealed a Distinct Mechanism of Aroma Bouquet Production

Gene-Metabolite Networks of Volatile Metabolism in Airen and Tempranillo Grape Cultivars Revealed a Distinct Mechanism of Aroma Bouquet Production

Metabolite Profiling of adh1 Mutant Response to Cold Stress in Arabidopsis

Dynamics of ADH and related genes responsible for the transformation of C6‐aldehydes to C6‐alcohols during the postharvest process of oolong tea

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Product

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Dynamics of ADH and related genes responsible for the transformation of C₆‐aldehydes to C₆‐alcohols during the postharvest process of oolong tea