Temperature and rates of protein degradation in the fish Gillichthys mirabilis

Somero, George N.; Doyle, Deborah

doi:10.1016/0305-0491(73)90085-0

Cited by 11 publications

(9 citation statements)

References 13 publications

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“…However, a subset of genes coding for ubiquitin-dependent proteolysis were up-regulated. Direct measurements of protein degradation rates in G. mirabilis gill tissues also showed an increase in the rate of proteolytic activity in warm-acclimated fish (Somero and Doyle, 1973). This discrepancy between increased proteolytic activity and decreased abundance of proteins involved in protein degradation might potentially be due to differences in tissues -gills versus hearts -tested in the two studies.…”

Section: Protein Turnovermentioning

confidence: 91%

“…A number of studies have demonstrated the thermal plasticity of G. mirabilis at the whole-organism, physiological, cellular and molecular levels (Sumner and Doudoroff, 1938;Somero and Doyle, 1973;Dietz and Somero, 1992;Hofmann, 2002, 2004;Somero, 2010, 2011). A recent study of cardiac activity in G. mirabilis (Jayasundara and Somero, 2013) revealed significant effects of acclimation on the thermal limits of cardiac function, particularly when fish were responding to an acute heat stress.…”

Section: Introductionmentioning

confidence: 99%

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Proteomic analysis of cardiac response to thermal acclimation in the eurythermal goby fishGillichthys mirabilis

Jayasundara

Tomanek

Dowd

et al. 2015

Journal of Experimental Biology

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Cardiac function is thought to play a central role in determining thermal optima and tolerance limits in teleost fishes. Investigating proteomic responses to temperature in cardiac tissues may provide insights into mechanisms supporting the thermal plasticity of cardiac function. Here, we utilized a global proteomic analysis to investigate changes in cardiac protein abundance in response to temperature acclimation (transfer from 13°C to 9, 19 and 26°C) in a eurythermal goby, Gillichthys mirabilis. Proteomic data revealed 122 differentially expressed proteins across acclimation groups, 37 of which were identified using tandem mass-spectrometry. These 37 proteins are involved in energy metabolism, mitochondrial regulation, iron homeostasis, cytoprotection against hypoxia, and cytoskeletal organization. Compared with the 9 and 26°C groups, proteins involved in energy metabolism increased in 19°C-acclimated fish, indicating an overall increase in the capacity for ATP production. Creatine kinase abundance increased in 9°C-acclimated fish, suggesting an important role for the phosphocreatine energy shuttle in cold-acclimated hearts. Both 9 and 26°C fish also increased abundance of hexosaminidase, a protein directly involved in posthypoxia stress cytoprotection of cardiac tissues. Cytoskeletal restructuring appears to occur in all acclimation groups; however, the most prominent effect was detected in 26°C-acclimated fish, which exhibited significantly increased actin levels. Overall, proteomic analysis of cardiac tissue suggests that the capacity to adjust ATP-generating processes is crucial to the thermal plasticity of cardiac function. Furthermore, G. mirabilis may optimize cellular functions at temperatures near 19°C, which lies within the species' preferred temperature range.

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Section: Protein Turnovermentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of cardiac response to thermal acclimation in the eurythermal goby fishGillichthys mirabilis

Jayasundara

Tomanek

Dowd

et al. 2015

Journal of Experimental Biology

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“…In its habitats, G. mirabilis experience temperatures betweeñ 5 and ~37°C, with daily fluctuations of ~12°C being common (Buckley and Hofmann, 2002;Buckley and Hofmann, 2004). A number of studies have demonstrated the thermal plasticity of G. mirabilis at different levels of biological organization, with recent studies focusing on changes in protein and gene expression in response to acclimation and acute thermal stress (Sumner and Doudoroff, 1938;Somero and Doyle, 1973;Dietz and Somero, 1992;Buckley and Hofmann, 2002;Buckley and Hofmann, 2004;Logan and Somero, 2010;Logan and Somero, 2011). This species' ability to tolerate a wide range of temperatures and its capacity to induce an acclimatory response at multiple levels of biological organization make it an ideal model for characterizing processes underlying physiological plasticity.…”

Section: Introductionmentioning

confidence: 99%

Physiological plasticity of cardiorespiratory function in a eurythermal marine teleost, the longjaw mudsucker,Gillichthys mirabilis

Jayasundara

Somero

2013

Journal of Experimental Biology

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SUMMARYAn insufficient supply of oxygen under thermal stress is thought to define thermal optima and tolerance limits in teleost fish. When under thermal stress, cardiac function plays a crucial role in sustaining adequate oxygen supply for respiring tissues. Thus, adaptive phenotypic plasticity of cardiac performance may be critical for modifying thermal limits during temperature acclimation. Here we investigated effects of temperature acclimation on oxygen consumption, cardiac function and blood oxygen carrying capacity of a eurythermal goby fish, Gillichthys mirabilis, acclimated to 9, 19 and 26°C for 4weeks. Acclimation did not alter resting metabolic rates or heart rates; no compensation of rates was observed at acclimation temperatures. However, under an acute heat ramp, warm-acclimated fish exhibited greater heat tolerance (CT max =33.3, 37.1 and 38.9°C for 9°C-, 19°C-and 26°C-acclimated fish, respectively) and higher cardiac arrhythmia temperatures compared with 9°C-acclimated fish. Heart rates measured under an acute heat stress every week during 28days of acclimation suggested that both maximum heart rates and temperature at onset of maximum heart rates changed over time with acclimation. Hemoglobin levels increased with acclimation temperature, from 35gl −1 in 9°C-acclimated fish to 60-80gl −1 in 19°C-and 26°C-acclimated fish. Oxygen consumption rates during recovery from acute heat stress showed post-stress elevation in 26°C-acclimated fish. These data, coupled with elevated resting metabolic rates and heart rates at warm temperatures, suggest a high energetic cost associated with warm acclimation in G. mirabilis. Furthermore, acclimatory capacity appears to be optimized at 19°C, a temperature shown by behavioral studies to be close to the speciesʼ preferred temperature. Supplementary material available online at

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“…A la suite de nombreuses études qualitatives de la synthèse protéique in vivo dans différents tissus des poissons, notamment dans le muscle (Das et Prosser, 1967 ;Jackim et Laroche, 1973 ;Somero et Doyle, 1973 ; Haschemeyer, 1974 ; Narayansingh et Eales, 1975), des déterminations quantitatives du phénomène de renouvellement des protéines ont été entreprises à l'aide des méthodes utilisées chez les Mammifères (Waterlow, Garlick et Millward, 1978 ; Blough, 1979). La méthode de décroissance appliquée chez la Carpe (Castilla, 1974 ;Bouche, 1975) et chez Gillichthys (Somero et Doyle, 1973) montre que la vitesse de dégradation des protéines dans le muscle de Poisson est légèrement plus lente que celle observée dans le foie et comparable à celle mesurée dans le muscle des Mammifères.…”

Section: Introductionunclassified

“…La méthode de décroissance appliquée chez la Carpe (Castilla, 1974 ;Bouche, 1975) et chez Gillichthys (Somero et Doyle, 1973) montre que la vitesse de dégradation des protéines dans le muscle de Poisson est légèrement plus lente que celle observée dans le foie et comparable à celle mesurée dans le muscle des Mammifères. La vitesse de synthèse des protéines musculaires mesurée à l'aide de la méthode d'infusion chez le muge et le baliste (Haschemeyer et Haschemeyer, Persell et (Ferrara, 1976).…”

Section: Introductionunclassified

Etude de la synthèse protéique in vivo dans le muscle de la truite arc-en-ciel (Salmo gairdneri R.). Influence de la température

Fauconneau¹,

Arnal²,

Luquet³

1981

Reprod. Nutr. Dévelop.

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Temperature and rates of protein degradation in the fish Gillichthys mirabilis

Cited by 11 publications

References 13 publications

Proteomic analysis of cardiac response to thermal acclimation in the eurythermal goby fishGillichthys mirabilis

Proteomic analysis of cardiac response to thermal acclimation in the eurythermal goby fishGillichthys mirabilis

Physiological plasticity of cardiorespiratory function in a eurythermal marine teleost, the longjaw mudsucker,Gillichthys mirabilis

Etude de la synthèse protéique in vivo dans le muscle de la truite arc-en-ciel (Salmo gairdneri R.). Influence de la température

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