Temperature- and pH-dependent accumulation of heat-shock proteins in the acidophilic green alga Chlamydomonas acidophila

Gerloff‐Elias, Antje; Barua, Deepak; Mölich, Andreas; Spijkerman, Elly

doi:10.1111/j.1574-6941.2006.00078.x

Cited by 44 publications

(29 citation statements)

References 48 publications

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“…It was suggested recently that heat-shock proteins play a major role in tolerance to pH and temperature stress among aquatic protists (Gerloff-Elias et al 2006), which may explain why eurythermal species are also tolerant to wide ranges of pH. Average μ and V reported in the present study were not different from the data obtained in response to temperature by Gächter & Weisse (2006), and the clones ranked similarly with respect to cell size and μ.…”

Section: Magnitude Of Intraspecific Variationcontrasting

confidence: 54%

Local adaptation among geographically distant clones of the cosmopolitan freshwater ciliate Meseres corlissi. II. Response to pH

Weisse

Scheffel²,

Štádler³

et al. 2007

Aquat. Microb. Ecol.

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We investigated the pH response of 5 clones of the oligotrichine ciliate Meseres corlissi originating from 2 temperate localities in Austria, from a subtropical habitat in China, and from a warm-temperate habitat in Australia. The pH reaction norm was investigated under standard laboratory conditions, with the small cryptophyte Cryptomonas sp. provided as food at saturating food levels over the pH range 4.0 to 9.5. Experiments were conducted at 22.5°C and lasted for 24 h. We measured growth rate, cell length and volume, and cellular production at each pH. We found significant clone-specific differences in each measured parameter, which increased with geographical distance of the isolates. Overall, the pH reaction norms of the Austrian isolates were significantly different from those of the Australian and Chinese clones. The tolerance to low pH differed by up to 1.5 pH units between the clones, i.e. intraspecific differences were comparable with interspecific differences measured earlier under similar experimental conditions. Our results suggest that the pH reaction norm is not homogenous for the species, but that genetically and phenotypically different ecotypes may exist among free-living, cosmopolitan ciliates that are adapted to a particular habitat.

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Section: Magnitude Of Intraspecific Variationcontrasting

confidence: 54%

Local adaptation among geographically distant clones of the cosmopolitan freshwater ciliate Meseres corlissi. II. Response to pH

Weisse

Scheffel²,

Štádler³

et al. 2007

Aquat. Microb. Ecol.

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“…The freshwater cryptophyte Cryptomonas sp., which is closely related to Rhodomonas marina investigated in the present study, tolerated wide pH fluctuations from pH 4.4 to 9.65 and was able to regulate its cell volume over this range (Weisse & Stadler 2006). Some common freshwater species of the green algal genus Chlamydomonas show positive growth rates over more than 7 pH units and keep their intracellular pH constant over this range (Spijkerman 2005, Gerloff-Elias et al 2006. In addition to affecting intracellular pH, low extracellular pH may directly affect membrane potential, energy partitioning, and enzyme activity (Beardall & Raven 2004, Riebesell 2004, Giordano et al 2005.…”

Section: Lower Ph Tolerance Limit For Marine Phytoplankton Growthmentioning

confidence: 89%

Effect of lowered pH on marine phytoplankton growth rates

Berge¹,

Daugbjerg²,

Andersen³

et al. 2010

Mar. Ecol. Prog. Ser.

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Continued anthropogenic carbon emissions are expected to result in an increase in atmospheric CO 2 concentration to 700 ppm by the end of this century. This will cause a corresponding drop in the global average surface water pH of the oceans by ~0.4 units to ~7.8 and an increase in the CO 2 concentration of seawater. Ocean acidification may potentially both stimulate and reduce primary production by marine phytoplankton. Data are scarce on the response of marine phytoplankton growth rates to lowered pH/increased CO 2 . Using the acid addition method to lower the seawater pH and manipulate the carbonate system, we determined in detail the lower pH limit for growth rates of 2 model species of common marine phytoplankton. We also tested whether growth and production rates of 6 other common species of phytoplankton were affected by ocean acidification (lowered to pH 7.0). The lower pH limits for growth of the dinoflagellate Heterocapsa triquetra and the cryptophyte Teleaulax amphioxeia were pH ~6.0 and 6.3, respectively. The growth rates of these 2 species were significantly reduced in the range of pH 6.4 to 6.5. Cell volume, growth, and production rates of the 6 other phytoplankton species were statistically similar in the pH range of ~7.0 to 8.5. Our results and literature reports on growth at lowered pH indicate that marine phytoplankton in general are resistant to climate change in terms of ocean acidification, and do not increase or decrease their growth rates according to ecological relevant ranges of pH and free CO 2 . We speculate about whether common natural pH fluctuations in time and space from 7.0 to 9.0 make phytoplankton capable of tolerating near-future ocean acidification. However, due to the less fluctuating pH environment of oceanic regions compared to coastal regions, truly oceanic species may be more sensitive to lowered pH than coastal species. KEY WORDS: Marine phytoplankton. Lowered pH . Growth rates . Primary production . Ocean acidification Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 416: [79][80][81][82][83][84][85][86][87][88][89][90][91] 2010 intracellular pH, membrane potential, energy partitioning, and enzyme activity (Beardall & Raven 2004, Riebesell 2004, Giordano et al. 2005. Thus, ocean acidification may reduce phytoplankton growth rates through direct pH effects.Experimental data on the effects of lowered pH and increased free CO 2 on growth and productivity of phytoplankton are few and do not show a clear pattern (Iglesias-Rodriguez et al. 2008, Langer et al. 2009). Some studies dealing with natural plankton communities, using micro-and mesocosms, have shown altered species composition in response to lowered pH and increased free CO 2 , while other studies have shown very limited effects on species composition and community production (Tortell et al. 2002, Kim et al. 2006, Feng et al. 2009. While microand mesocosm studies have the advantage of allowing the analyses of complete, natural phytoplankton communities, the ...

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“…Especially, when external pH is very low and internal pH is neutral the proton gradient over the cytoplasm membrane will drive CO 2 accumulation. A large proton gradient was present here: external pH was 2.4 and internal pH was either 7.6 (Gerloff- Elias et al 2006) or ranged between 6.2 and 6.8 (Suppl. Fig.…”

Section: Extent Of Ccmmentioning

confidence: 96%

The expression of a carbon concentrating mechanism in Chlamydomonas acidophila under variable phosphorus, iron, and CO2 concentrations

Spijkerman

2011

Photosynth Res

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The CO(2) acquisition was analyzed in Chlamydomonas acidophila at pH 2.4 in a range of medium P and Fe concentrations and at high and low CO(2) condition. The inorganic carbon concentrating factor (CCF) was related to cellular P quota (Q(p)), maximum CO(2)-uptake rate by photosynthesis (V(max,O2)), half saturation constant for CO(2) uptake (K(0.5)), and medium Fe concentration. There was no effect of the medium Fe concentration on the CCF. The CCF increased with increasing Q(p) in both high and low CO(2) grown algae, but maximum Q(p) was 6-fold higher in the low CO(2) cells. In high CO(2) conditions, the CCF was low, ranging between 0.8 and 3.5. High CCF values up to 9.1 were only observed in CO(2)-limited cells, but P- and CO(2)-colimited cells had a low CCF. High CCF did not relate with a low K(0.5) as all CO(2)-limited cells had a low K(0.5) (<4 μM CO(2)). High C(i)-pools in cells with high Q(p) suggested the presence of an active CO(2)-uptake mechanism. The CCF also increased with increasing V(max,O2) which reflect an adaptation to the nutrient in highest demand (CO(2)) under balanced growth conditions. It is proposed that the size of the CCF in C. acidophila is more strongly related to porter density for CO(2) uptake (reflected in V(max,O2)) and less- to high-affinity CO(2) uptake (low K(0.5)) at balanced growth. In addition, high CCF can only be realized with high Q(p).

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Temperature- and pH-dependent accumulation of heat-shock proteins in the acidophilic green alga Chlamydomonas acidophila

Cited by 44 publications

References 48 publications

Local adaptation among geographically distant clones of the cosmopolitan freshwater ciliate Meseres corlissi. II. Response to pH

Local adaptation among geographically distant clones of the cosmopolitan freshwater ciliate Meseres corlissi. II. Response to pH

Effect of lowered pH on marine phytoplankton growth rates

The expression of a carbon concentrating mechanism in Chlamydomonas acidophila under variable phosphorus, iron, and CO2 concentrations

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