Temperature and GA3 as Modulating Factors in the Biosynthesis of Alkaloids during Imbibition and Early Development of Annona x atemoya Mabb. cv. ‘Gefner’ Seedlings

Silva, Gustavo Cabral da; De-la-Cruz-Chacón, Iván; Honório, Ana Beatriz Marques; Martin, Bruna Cavinatti; Sousa, Marília Caixeta; Campos, Felipe Girotto; Boaro, Carmen Sílvia Fernandes; Ferreira, Gisela

doi:10.3390/horticulturae8090766

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…The need to allocate defensive compounds to a particular plant part primarily depends on its susceptibility to predators but also varies with the developmental stage of the plant and environmental conditions [37]. The biosynthesis of alkaloids is often affected by temperature, which also influences respiration and photosynthesis [38]. Enhanced respiration and photosynthesis may lead to elevated protein synthesis and alkaloid production [38].…”

Section: Discussionmentioning

confidence: 99%

“…The biosynthesis of alkaloids is often affected by temperature, which also influences respiration and photosynthesis [38]. Enhanced respiration and photosynthesis may lead to elevated protein synthesis and alkaloid production [38]. Despite the seasonal variation, Konchar et al [34] pointed out that the reproductive stage of the plant is more consistent with the observed differences in alkaloid accumulation than any variation in the environment.…”

Section: Discussionmentioning

confidence: 99%

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Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime

Marković,

Trifunović-Momčilov,

Radulović

et al. 2023

Horticulturae

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The genus Fritillaria is characterized by the production of alkaloids, primarily of the isosteroidal type. The aim of this preliminary study was to perform a histolocalization screening aimed at revealing the presence of alkaloids in the bulbs of F. meleagris, cultured in vitro under different growth regimes and at different developmental stages, as a powerful tool to identify preferable in vitro plant material for alkaloid isolation. Histochemical localization of alkaloids was performed using Wagner’s and Dragendorff’s reagents in fresh sections of bulbs cultured at 24 °C or 7 °C for 4 weeks, as well as those cultured at 24 °C following the 4-week chilling treatment, which were sampled at the beginning of sprouting. A positive reaction was observed with both reagents and was particularly intense in cold-treated bulbs. Alkaloids were mainly distributed in the bulbs and partly in the sprouts of the in vitro grown F. meleagris. The most intense staining, indicative of high alkaloid content, was observed in the bulb scales of pre-chilled bulbs that sprouted at 24 °C, rendering them preferable as in vitro plant material for alkaloid isolation. The results suggest that alkaloid production in the bulbs of F. meleagris can be improved by manipulating growth in the microenvironment of in vitro cultures, in order to meet the increasing industrial demand for medicinally or commercially important metabolites used as traditional medicines and herbal remedies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime

Marković,

Trifunović-Momčilov,

Radulović

et al. 2023

Horticulturae

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Climate change-related warming-induced shifts in leaf chemical traits favor nutrition of the specialist herbivore Battus polydamas archidamas

et al. 2023

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One of the major impacts of climate change is increasing global temperatures. Because warming is expected to affect plant morphological and chemical traits, it may therefore also influence plant interactions with other trophic levels, including herbivores. Here, we simulated a climate warming scenario of +2.7°C in the field using open-top chambers and assessed the effects of warming on plant performance (growth, leaf area, and chlorophyll), leaf nutrients (nitrogen and carbon), and primary (amino acids and carbohydrates) and secondary (toxic aristolochic acids) metabolites in the plant Aristolochia chilensis. We performed untargeted metabolomics analyses for estimating general changes in foliar metabolites between ambient control and warming-treated plants. Bioassays were additionally conducted to evaluate how changes in host plant chemistry affected growth and nutritional parameters in first-instar larvae of the specialist lepidopteran herbivore Battus polydamas archidamas. We found that warming did not significantly affect plant performance, but did result in significant changes in leaf nutrients, and primary and secondary metabolites, although in opposite directions. While primary metabolites (specifically, nitrogen-containing compounds) decreased in response to treatment, aristolochic acids increased. Untargeted metabolomics analyses showed that, of a total of 824 features, 50 were significantly different between ambient control and warming-treated plants; some of these were identified by MS/MS spectra as amino acids. Larvae feeding on warming-treated plants, showed significantly enhanced growth, food conversion efficiency, and lipid concentration. Our study contributes to current understanding of climate change impacts on trophic interactions, showing that projected temperature increases lead to changes in the resistance phenotype of the host plant, favoring nutrition and growth of a unique specialist herbivore.

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Gibberellic Acid Concentrations and Storage of Caryocar brasiliense (Caryocaraceae) Seeds Propagated in Tubes

et al. 2022

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The immersion of seeds in gibberellic acid solutions can promote dormancy breaking. Storage can be dynamic in seed longevity. The objective of this work was to evaluate different concentrations of gibberellic acid applied to seeds isolated from two storage times on the emergence of C. brasiliense tree seedlings grown in tubes. There were two experiments with different planting and storage times. The seeds were extracted from the endocarp. In both, a randomized block design was used with the following seven treatments: 0, 50, 100, 500, 1000 and 2000 mg L−1 of GA3 and dry seeds without pre-soaking, with five replications of 20 seeds. The seeds were treated with fungicide and sown in tubes containing substrate. After 60 days, evaluations and transplantation were carried out. In the experiment with older pyrenes (seeds stored for seven months), there was high seed mortality (96.54%) and a small emergence rate (3.45%). On the other hand, when new pyrenes were used, the percentage of dead seeds was 32.71% and emergence was 62.28%. GA3 did not stimulate germination or the diameter of C. brasiliense tree seedlings, except for stem length. The 2000 concentration provided a greater stem length and greater leaf area compared to dry seeds.

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Temperature and GA3 as Modulating Factors in the Biosynthesis of Alkaloids during Imbibition and Early Development of Annona x atemoya Mabb. cv. ‘Gefner’ Seedlings

Cited by 3 publications

References 32 publications

Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime

Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime

Climate change-related warming-induced shifts in leaf chemical traits favor nutrition of the specialist herbivore Battus polydamas archidamas

Gibberellic Acid Concentrations and Storage of Caryocar brasiliense (Caryocaraceae) Seeds Propagated in Tubes

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