Temperature affects morphology, physiology, and biochemistry of plug seedlings of Astragalus membranaceus

Ren, Xiuxia; Ya, Liu; Jeong, Hai Kyoung; Soundararajan, Prabhakaran; Jeong, Byoung Ryong

doi:10.1007/s11738-018-2799-0

Cited by 7 publications

(4 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Temperature is a major environmental factor that regulates various aspects of plant morphology, physiology, and biochemistry, affecting germination, growth, development, and flowering (Ren et al, 2019). Fertility in crops such as rapeseed (Yu et al, 2015) and rice (Oryza sativa) (Yu et al, 2017) is affected by temperature.…”

Section: Introductionmentioning

confidence: 99%

Investigation for a multi-silique trait in Brassica napus by alternative splicing analysis

Chai

Zhang

et al. 2020

PeerJ

View full text Add to dashboard Cite

Background Flower and fruit development are vital stages of the angiosperm lifecycle. We previously investigated the multi-silique trait in the rapeseed (Brassica napus) line zws-ms on a genomic and transcriptomic level, leading to the identification of two genomic regions and several candidate genes associated with this trait. However, some events on the transcriptome level, like alternative splicing, were poorly understood. Methods Plants from zws-ms and its near-isogenic line (NIL) zws-217 were both grown in Xindu with normal conditions and a colder area Ma’erkang. Buds from the two lines were sampled and RNA was isolated to perform the transcriptomic sequencing. The numbers and types of alternative splicing (AS) events from the two lines were counted and classified. Genes with AS events and expressed differentially between the two lines, as well as genes with AS events which occurred in only one line were emphasized. Their annotations were further studied. Results From the plants in Xindu District, an average of 205,496 AS events, which could be sorted into five AS types, were identified. zws-ms and zws-217 shared highly similar ratios of each AS type: The alternative 5′ and 3′ splice site types were the most common, while the exon skipping type was observed least often. Eleven differentially expressed AS genes were identified, of which four were upregulated and seven were downregulated in zws-ms. Their annotations implied that five of these genes were directly associated with the multi-silique trait. While samples from colder area Ma’erkang generated generally reduced number of each type of AS events except for Intron Retention; but the number of differentially expressed AS genes increased significantly. Further analysis found that among the 11 differentially expressed AS genes from Xindu, three of them maintained the same expression models, while the other eight genes did not show significant difference between the two lines in expression level. Additionally, the 205 line-specific expressed AS genes were analyzed, of which 187 could be annotated, and two were considered to be important. Discussion This study provides new insights into the molecular mechanism of the agronomically important multi-silique trait in rapeseed on the transcriptome level and screens out some environment-responding candidate genes.

show abstract

Section: Introductionmentioning

confidence: 99%

Investigation for a multi-silique trait in Brassica napus by alternative splicing analysis

Chai

Zhang

et al. 2020

PeerJ

View full text Add to dashboard Cite

show abstract

“…However, A. membranaceus is cold-and drought-resistant but is sensitive to high temperatures. The root and shoot mass, stem diameter, chlorophyll level, carbohydrate and protein accumulation of seedlings grown at 25 C were significantly higher (Ren et al, 2019)…”

Section: Introductionmentioning

confidence: 90%

“…However, A. membranaceus is cold‐ and drought‐resistant but is sensitive to high temperatures. The root and shoot mass, stem diameter, chlorophyll level, carbohydrate and protein accumulation of seedlings grown at 25°C were significantly higher (Ren et al, 2019). Plant height, root activity and photosynthetic activity are severely restricted when the ambient temperature is >40°C.…”

Section: Introductionmentioning

confidence: 99%

Dark septate endophyte inoculation enhances antioxidant activity in Astragalus membranaceus var. mongholicus under heat stress

Bi,

Xue

2023

Physiologia Plantarum

View full text Add to dashboard Cite

The influence of dark septate endophytic (DSE) on the antioxidant activity of Astragalus membranaceus var. mongholicus under heat stress was investigated. A. membranaceus plants, with or without DSE inoculation, were grown at 28°C for 8 weeks in a greenhouse and subsequently subjected to heat stress conditions (42°C) in an artificial climate chamber. DSE inoculation significantly decreased the malondialdehyde (MDA) content during the initial three days of heat stress. The activities of superoxide dismutase (SOD) and peroxidase (POD) of A. membranaceus leaves were significantly enhanced by DSE inoculation under heat stress, with SOD activities being 63–81% higher than in other treatments. The glutathione (GSH) and putrescine (Put) contents accumulated significantly on the third day under heat stress with DSE inoculation. Additionally, the contents of soluble sugars and proline (Pro) exhibited significant increases on the seventh day of heat stress and were 33–55% and 81–83% higher than in other treatments, respectively. Three‐way ANOVA shows that DSE inoculation under heat stress exerted a significant impact on MDA. Multivariate linear regression and structural equality modelling (SEM) further show that the interaction among these antioxidants significantly decreased MDA content and maintained the normal function of cell membranes. In conclusion, DSE inoculation enhanced the heat tolerance of A. membranaceus by boosting its antioxidant capacity and reducing MDA production. This study highlights the potential of utilizing DSE as a strategy to enhance plant heat tolerance.

show abstract

“…Temperate is a major environmental factor that regulates various aspects of plant morphology, physiology, and biochemistry, affecting germination, growth, development, and flowering (Ren et al, 2019). Fertility in crops such as rapeseed (Yu et al, 2015) and rice (Oryza sativa) (Yu et al, 2017) is affected by temperature.…”

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "Investigation for a multi-silique trait in Brassica napus by alternative splicing analysis (v0.1)"

Liu¹

2020

View full text Add to dashboard Cite

Background. Flower and fruit development are vital stages of the angiosperm lifecycle. We previously investigated the multi-silique trait in the rapeseed (Brassica napus) line zws-ms on a genomic and transcriptomic level, leading to the identification of two genomic regions and several candidate genes associated with this trait. However, some events on transcriptome level, like alternative splicing, were poorly understood. Methods. Plants from zws-ms and its near-isogenic line (NIL) zws-217 were both grown in Xindu with normal conditions and a colder area Ma'erkang. Buds from the two lines were sampled and RNA was isolated to perform the transcriptomic sequencing. The numbers and types of alternative splicing (AS) events from the two lines were counted and classified. Genes with AS events and expressed differentially between the two lines, as well as genes with AS events which occurred in only one line were emphasized. Their annotations were further studied. Results. From the plants in Xindu District, an average of 205,496 AS events, which could be sorted into 5 AS types, were identified. zws-ms and zws-217 shared highly similar ratios of each AS type: The alternative 5' and 3' splice site types were the most common, while the exon skipping type was observed least often. Eleven differentially expressed AS genes were identified, of which four were upregulated and seven were downregulated in zws-ms. Their annotations implied that five of these genes were directly associated with the multi-silique trait. While samples from colder area Ma'erkang generated generally reduced number of each type of AS events except the Intron Retention; but the number of differentially expressed AS genes increased significantly. Further analysis found that among the 11 differentially expressed AS genes from Xindu, three of them maintained the same expression models, while the other 8 genes did not show significant difference between the two lines in expression level. Additionally, the 205 line-specifically expressed AS genes were analyzed, of which 187 could be annotated, and two were considered to be important. Discussion. This study provides new insights into the molecular mechanism of the agronomically important multi-silique trait in rapeseed on transcriptome level and screens out some environmentresponding candidate genes.

show abstract

Temperature affects morphology, physiology, and biochemistry of plug seedlings of Astragalus membranaceus

Cited by 7 publications

References 51 publications

Investigation for a multi-silique trait in Brassica napus by alternative splicing analysis

Investigation for a multi-silique trait in Brassica napus by alternative splicing analysis

Dark septate endophyte inoculation enhances antioxidant activity in Astragalus membranaceus var. mongholicus under heat stress

Peer Review #1 of "Investigation for a multi-silique trait in Brassica napus by alternative splicing analysis (v0.1)"

Contact Info

Product

Resources

About