Temperature affects liver and muscle metabolism in photostimulated migratory redheaded buntings (Emberiza bruniceps)

Sur, Sayantan; Sharma, Aakansha; Trivedi, Amit Kumar; Bhardwaj, Sanjay Kumar; Kumar, Vinod

doi:10.1007/s00360-019-01229-5

Cited by 7 publications

(3 citation statements)

References 51 publications

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“…The product of this reaction ( postassessment of RNA integrity) was used for qPCR. Gene-specific primers were designed from gene sequences available with us (acc, fasn, dgat2, cd36, fabp3, cpt1, atp2a2, sirt1, hspa8, sod1, nos2 and adcyap1;Trivedi et al, 2014;Sharma et al, 2018a,b;Sharma and Kumar, 2019;Sur et al, 2019Sur et al, , 2020 or from those we cloned for this study (apoa1, vps13a, camk1 and camk2a). We cloned a gene sequence by using degenerate primer sequence based on a conserved region of the gene, and the amplified products (>500 bp) of bunting hypothalamus cDNA templates were commercially sequenced (Eurofins, Bangalore, India) and subjected to a NCBI database nucleotide BLAST search to ascertain gene identity.…”

Section: Measurement Of Gene Expression In the Liver Muscle And Hypothalamusmentioning

confidence: 99%

Molecular changes associated with migratory departure from wintering areas in obligate songbird migrants

Sharma

Singh

Gupta

et al. 2021

Journal of Experimental Biology

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Day length regulates the development of spring migratory and subsequent reproductive phenotypes in avian migrants. This study used molecular approaches, and compared mRNA and proteome-wide expression in captive redheaded buntings that were photostimulated under long-day (LD) conditions for 4 days (early stimulated, LD-eS) or for ∼3 weeks until each bird had shown 4 successive nights of Zugunruhe (stimulated, LD-S); controls were maintained under short days. After ∼3 weeks of LD, photostimulated indices of the migratory preparedness (fattening, weight gain and Zugunruhe) were paralleled with upregulated expression of acc, dgat2 and apoa1 genes in the liver, and of cd36, fabp3 and cpt1 genes in the flight muscle, suggesting enhanced fatty acid (FA) synthesis and transport in the LD-S state. Concurrently, elevated expression of genes involved in the calcium ion signalling and transport (camk1 and atp2a2; camk2a in LD-eS), cellular stress (hspa8 and sod1, not nos2) and metabolic pathways (apoa1 and sirt1), but not of genes associated with migratory behaviour (adcyap1 and vps13a), were found in the mediobasal hypothalamus (MBH). Further, MBH-specific quantitative proteomics revealed that out of 503 annotated proteins, 28 were differentially expressed (LD-eS versus LD-S: 21 up-regulated and 7 down-regulated) and they enriched five physiological pathways that are associated with FA transport and metabolism. These first comprehensive results on gene and protein expression suggest that changes in molecular correlates of FA transport and metabolism may aid the decision for migratory departure from wintering areas in obligate songbird migrants.

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Section: Measurement Of Gene Expression In the Liver Muscle And Hypothalamusmentioning

confidence: 99%

Molecular changes associated with migratory departure from wintering areas in obligate songbird migrants

Sharma

Singh

Gupta

et al. 2021

Journal of Experimental Biology

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“…We assayed the accumulation of neutral lipids in the liver as a measure of flight fuel storage with the development of spring and autumn migration phenotypes. For this, we used the Oil Red O (ORO) staining protocol, as described by Mehlem, Hagberg, Muhl, Eriksson, and Falkevall (2013) and demonstrated in red-headed buntings (Sur, Sharma, Trivedi, Bhardwaj, & Kumar, 2019). Briefly, frozen liver tissue was embedded in 15% polyvinylpyrrolidone (PVP; PVP40T, Sigma) and sectioned at 15 µm thickness on a Leica 1850 Cryostat.…”

Section: Assay Of Flight Fuel Accumulation In the Liver And Adipose Tmentioning

confidence: 99%

Metabolic plasticity mediates differential responses to spring and autumn migrations: Evidence from gene expression patterns in migratory buntings

Sharma

Kumar

2019

Experimental Physiology

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New Findings What is the central question of this study?What are the molecular underpinnings of seasonal metabolic plasticity during spring and autumn migrations in songbirds? What is the main finding and its importance?We report differences in mRNA levels of genes involved in the regulation of glucose and fat metabolism between photoinduced non‐migratory and migratory states and between the spring and autumn migratory states. Higher expression of genes associated with fat mobilization and energy generation in the spring than in the autumn migration suggests differential activation of the metabolic pathways or alteration in the efficiency of existing functional machinery during annual journeys between nearly fixed destinations. Abstract The molecular underpinnings of metabolic plasticity underlying differential responses to spring and autumn migrations are not well understood. We investigated this by examining the differences in mRNA levels of metabolic genes in the liver, muscle and adipose tissues of night‐migratory red‐headed buntings between photostimulated non‐migratory and migratory states and between spring and autumn migratory states. Buntings accumulated more subcutaneous fat and hepatic lipid, had higher body mass, larger adipose cells and higher circulating triglyceride and free fatty acid levels and exhibited more intense Zugunruhe in the spring migratory state than in the autumn migratory state. More importantly, we found differences in the hepatic expression of pdc and pdk genes, indicating a differential acetyl‐CoA requirement, and of the mdh and ogdh genes, suggesting differential oxidative phosphorylation between the non‐migratory and migratory states and between the spring and autumn migratory states. Differences in fasn, bmal1 and glut1 mRNA levels were consistent with this and suggested seasonal differences in lipogenesis and/or glucose uptake. Likewise, differences in mRNA levels of genes coding for lipases (atgl and lpl) suggested that adipose triglycerides and free fatty acids serve largely as the metabolic substrate. Furthermore, changes in mRNA levels of genes coding for the fatty acid binding protein (fabp3) and fatty acid translocases (cd36) were consistent with differential fat fuel supply (via circulating free fatty acids) to aerobically exercising flight muscles between the spring and autumn migrations. These results show seasonal adaptation of genetic pathway(s) underlying seasonal metabolic plasticity that seems to mediate differential responses to spring and autumn migrations in latitudinal migratory songbirds.

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“…A combined stress due to salinity and temperature seriously affect osmoregulation in European sea bass Dicentrarchus labrax (Masroor et al, 2018). Temperature variations affect thermal tolerance (Shaughnessy & McCormick, 2018), rate of oxygen consumption, feeding, liver and muscle metabolism (Sur et al, 2019) and growth in marine and aquatic animals (Magnoni et al, 2018).…”

Section: Thermoregulatory Markersmentioning

confidence: 99%

Markers for Global Climate Change and Its Impact on Social, Biological and Ecological Systems: A Review

Upadhyay¹

2020

AJCC

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Present article sketches out major climate induced changes in marine, aquatic and terrestrial life. Few important biomarkers such as ecological, meteorological, socioeconomic, thermal, biophysical and biological, behavioral markers of climate change and global environmental stress have been highlighted to predict the future challenges and finding appropriate solutions. Though, so many climate change induced effects are visible but few unpredictable effects may be seen in future. Therefore, all such effects have been acknowledged, and tried to find appropriate solutions. Most visible effect is collection of high amounts of carbon dioxide in the atmosphere which is responsible for green house effect and causing natural calamities round the globe. It is not only jeopardized the survival of terrestrial, fresh water animals mainly planktons, bottom dwellers; coral reefs, algae, fish fauna in marine environment belong to different taxon but also responsible for disruption of ocean's food web due to non-assimilation of extra carbon dioxide by the ocean water. There is a sharp decline in fresh water and sea shore micro-flora and micro-fauna. Other major visible effects are loss of biodiversity, depletion of forests, land degradation, severe floods and draughts. On other hand sudden changes in weather conditions causing irreparable devastations due to hurricanes and typhoons, storms, lightening, earthquakes and tsunamis are normally on rise. Both economic and ecological breakdowns are occurring more frequently which are more impactful and persistent. Climate change is major human health stressor; it is making fragmentation of socio-cultural bonds and reducing fertility of soil finally crop production. Climate change is imposing nonadaptive forced human migration, territorial conflicts, decreasing ecosystem productivity, disease out breaks, and impelling unequal resource utilization.

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Temperature affects liver and muscle metabolism in photostimulated migratory redheaded buntings (Emberiza bruniceps)

Cited by 7 publications

References 51 publications

Molecular changes associated with migratory departure from wintering areas in obligate songbird migrants

Molecular changes associated with migratory departure from wintering areas in obligate songbird migrants

Metabolic plasticity mediates differential responses to spring and autumn migrations: Evidence from gene expression patterns in migratory buntings

Markers for Global Climate Change and Its Impact on Social, Biological and Ecological Systems: A Review

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