Temozolomide may induce cell cycle arrest by interacting with URG4/URGCP in SH-SY5Y neuroblastoma cells

Abstract

Temozolomide (TMZ) is an alkylating drug used usually in glioma treatment by inducing the apoptosis in glioma cell. The aim of the study is to investigate the anticancer mechanism of TMZ in SH-SY5Y human neuroblastoma cell line. Cytotoxic effects of TMZ were determined by using XTT assay. IC50 doses in the SH-SY5Y were detected as 5 mM. Expression profiles of novel genes URG4/URGCP, CCND1, CCND2, CDK4, and BCL2 were determined by real-time PCR. The apoptotic effects of TMZ were evaluated with TUNEL method. Furthermore, effects of TMZ on colony formation and invasion were investigated in this study. It was observed that TMZ in SH-SY5Y cell line caused a significant decrease in the gene expressions of URG4/URGCP, CCND1, CCND2, CDK4, and BCL2. According to TUNEL assay results, TMZ markedly induced apoptosis in SH-SY5Y cell line. It was found that TMZ in SH-SY5Y cell line suppressed invasion and colony formation using matrigel invasion chamber and colony formation assay, respectively. To conclude, it is thought that TMZ demonstrates anticarcinogenesis activity by affecting cell cycle arrest, apoptosis, invasion, and colony formation on SH-SY5Y cells. TMZ may be an effective agent for treatment of neuroblastoma as a single or in combination with other drugs.

A New Epigenetic Mechanism of Temozolomide Action in Glioma Cells

“…Incubation with TMZ in a concentration range 1 – 2000uM up to 48h resulted in a decrease of cell viability up to ~16% in HeLa and U118 cells. Our observation is consistent with other reports [ 30 – 32 ]. Moreover it has been also shown that TMZ cytotoxicity is not due to induction of apoptosis [ 31 – 32 ].…”

A new epigenetic mechanism of temozolomide action in glioma cells

“…Incubation with TMZ in a concentration range 1 -2000uM up to 48h resulted in a decrease of cell viability up to~16% in HeLa and U118 cells. Our observation is consistent with other reports [30][31][32]. Moreover it has been also shown that TMZ cytotoxicity is not due to induction of apoptosis [31][32].…”

In vitro Comparative Study for Anti-proliferative Activity of Some Plant Extracts, Fam. Apiaceae, on HeLa Cell Line

“…Alteration in gene expression implies cell arresting and being forced to apoptosis which ceases cancer progress. (Çıtışlı et al, 2015;Fahrioğlu et al, 2016). Cilantro act as a protective agent in lipid metabolism of colon cancer when studied on rats, as it reduced the cholesterol and cholesterol to phospholipid ratio while increased phospholipid ratio (Chithra et al, 2000).…”