Temnopleurus as an emerging echinoderm model

Yaguchi, Shunsuke

doi:10.1016/bs.mcb.2018.09.001

Cited by 5 publications

(12 citation statements)

References 8 publications

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“…Echinoderms with similar developmental tempos include the Panamanian populations of L. variegatus which have generation times on the order of 6 to 8 months, 57 comparable to L. pictus . Temnopleurus reevesii can also achieve maturity between 6 and 10 months 58 under optimal conditions, making its generation time similar to L. pictus . Those working with Paracentrotus lividus have started to compile similar staging schemes 59 and it may also have comparable generation times of approximately 5 months to earliest gamete production 28 .…”

Section: Discussionmentioning

confidence: 99%

“…68 Gene editing has now become widespread in marine organisms, including sea urchins. [69][70][71][72][73] During this "CRISPR era" [74][75][76] the ability to rear juveniles, generate lines 58 and investigate later life developmental impacts resulting from early events in embryogenesis is going to be of increasing importance. The comparatively short generation time of L. pictus enables opportunities to create inbred lines of animals with reduced variability and stable genetic backgrounds for manipulation.…”

Section: Conclusion: Historical and Future Contributions From Resementioning

confidence: 99%

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Embryo, larval, and juvenile staging of Lytechinus pictus from fertilization through sexual maturation

Nesbit

Hamdoun

2020

Developmental Dynamics

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Background: Sea urchin embryos have been used for more than a century in the study of fertilization and early development. However, several of the species used, such as Strongylocentrotus purpuratus, have long generation times making them suboptimal for transgenerational studies. Results: Here, we present an overview of the development of a rapidly developing echinoderm species, Lytechinus pictus, from fertilization through sexual maturation. When grown at room temperature (20 C) embryos complete the first cell cycle in 90 minutes, followed by subsequent cleavages every 45 minutes, leading to hatching at 9 hours postfertilization (hpf). The swimming embryos gastrulate from 12 to 36 hpf and produce the cells which subsequently give rise to the larval skeleton and immunocytes. Larvae begin to feed at 2 days and metamorphose by 3 weeks. Juveniles reach sexual maturity at 4 to 6 months of age, depending on individual growth rate. Conclusions: This staging scheme lays a foundation for future studies in L. pictus, which share many of the attractive features of other urchins but have the key advantage of rapid development to sexual maturation. This is significant for multigenerational and genetic studies newly enabled by CRISPR-CAS mediated gene editing.

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Section: Discussionmentioning

confidence: 99%

Section: Conclusion: Historical and Future Contributions From Resementioning

confidence: 99%

Embryo, larval, and juvenile staging of Lytechinus pictus from fertilization through sexual maturation

Nesbit

Hamdoun

2020

Developmental Dynamics

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“…Because the adults are carnivorous, they have to be kept individually if the aquarium is not large enough. They are fed with mussels, clams, and oysters as described previously (Yaguchi, 2019a). This species has not been frequently used as a biological material, but the Kitazawa group has reported details of their late development along with another member of the Temnopleurus genus.…”

Section: Temnopleurus Reevesii Has the Potential To Be A Sea Urchin Genetics Modelmentioning

confidence: 99%

“…Although the breeding season of T . reevesii under natural conditions has not been reported thus far, gametes are constantly obtained when the temperature of the seawater in the adult aquarium is set above 20°C (Yaguchi, 2019a), suggesting that their breeding season is summer‐fall. However, based on our trials, the culturing temperature for embryos/larvae can be between 15–30°C for T .…”

Section: Temnopleurus Reevesii Has the Potential To Be A Sea Urchin G...mentioning

confidence: 99%

“…After compaction occurs at this stage, development proceeds as in other sea urchins, resulting in pluteus larvae with similar morphology to other sea urchin larvae including normal spicule patterns (Figure 2e‐H). Temperature tolerance levels has been established during culturing of the embryos/larvae of this species (Yaguchi, 2019a; Yaguchi et al., 2015). Generally, the culturing temperature is set to less than 20°C for sea urchins whose breeding season is winter‐spring and 20–25°C for sea urchins whose breeding season is summer‐fall.…”

Section: Temnopleurus Reevesii Has the Potential To Be A Sea Urchin G...mentioning

confidence: 99%

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Temnopleurus reevesii as a new sea urchin model in genetics

Yaguchi

2022

Dev Growth Differ

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Echinoderms, including sea urchins and starfish, have played important roles in cell, developmental and evolutionary biology research for more than a century. However, since most of them take a long time to mature sexually and their breeding seasons are limited, it has been difficult to obtain subsequent generations in the laboratory, resulting in them not being recognized as model organisms in recent genetics research. To overcome this issue, we maintained and obtained gametes from several nonmodel sea urchins in Japan and finally identified Temnopleurus reevesii as a suitable model for sea urchin genetics. Genomic and transcriptomic information was obtained for this model, and the DNA database TrBase was made publicly available. In this review, we describe how we found this species useful for biological research and show an example of CRISPR/Cas9 based knockout sea urchin.

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Staging of post-settlement growth in the nudibranch Hypselodoris festiva

Hayashi,

Nakano

2024

Sci Rep

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Sea slugs of the family Chromodorididae (Nudibranchia, Gastropoda, Mollusca) have garnered attention by researchers and hobbyists alike for their bright and variable color patterns. However, the chromodorid life cycle has yet to be fully elucidated as there exist no reports of their rearing in the laboratory. Here, we report the rearing of Hypselodoris festiva from eggs to adults, where we categorized their post-settlement growth patterns from juvenile to adult stages. Body coloration appeared around 36 days, and organogenesis of vital adult organs began within 42 days after hatching. The anus of H. festiva was observed to change from a ventral to dorsal position during juvenile growth. Individuals reached sexual maturity after six months post-hatching, with successful mating and spawning observed ex situ. This study outlines comprehensive rearing methods and life cycle staging that could be applied to other chromodorid species. We propose H. festiva as a model organism for chromodorid research, with this research contributing to the progress of developmental and evolutionary research on sea slugs.

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Temnopleurus as an emerging echinoderm model

Cited by 5 publications

References 8 publications

Embryo, larval, and juvenile staging of Lytechinus pictus from fertilization through sexual maturation

Embryo, larval, and juvenile staging of Lytechinus pictus from fertilization through sexual maturation

Temnopleurus reevesii as a new sea urchin model in genetics

Staging of post-settlement growth in the nudibranch Hypselodoris festiva

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