TEM Gene Detection in Clinical Pseudomonas aeruginosa and Escherichia coli Samples

Shams, Elahe; Nateghi, Behnaz; Eshaghiyan, Amir; Behshood, Parisa

doi:10.18502/rmm.v7i1.5258

Cited by 3 publications

(2 citation statements)

References 7 publications

(7 reference statements)

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“…In our study, amplicon sequencing of bla CTX-M revealed the predominance of bla CTX-M-15 (5/6, 83%) among the CTX-resistant E. coli isolates. These findings are in line with the fact that bla CTX-M-15 is the most widely spread ESBL genotype globally [ 34 ]. The emergence of bla CTX-M-15 has been attributed to the clonal spread of the E. coli O25b:H4-ST131 pandemic clone.…”

Section: Discussionsupporting

confidence: 79%

Detection of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Enterobacteriaceae from Diseased Broiler Chickens in Lusaka District, Zambia

Chileshe,

Shawa,

Phiri

et al. 2024

Antibiotics

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Poultry products in Zambia form an integral part of the human diet in many households, as they are cheap and easy to produce. The burden of poultry diseases has, however, remained a major challenge. Growing consumer demand for poultry products in Zambia has resulted in non-prudent antimicrobial use on farms, intending to prevent and treat poultry diseases for growth optimisation and maximising profits. This cross-sectional study aimed to identify the different types of bacteria causing diseases in chickens in Lusaka and to detect the extended-spectrum lactamase (ESBL)-encoding genes. We collected 215 samples from 91 diseased chickens at three post-mortem facilities and screened them for Gram-negative bacteria. Of these samples, 103 tested positive for various clinically relevant Enterobacteriaceae, including Enterobacter (43/103, 41.7%), Escherichia coli (20/103, 19.4%), Salmonella (10/103, 9.7%), and Shigella (8/103, 7.8%). Other isolated bacteria included Yersinia, Morganella, Proteus, and Klebsiella, which accounted for 21.4%. E. coli, Enterobacter, Salmonella, and Shigella were subjected to antimicrobial susceptibility testing. The results revealed that E. coli, Enterobacter, and Shigella were highly resistant to tetracycline, ampicillin, amoxicillin, and trimethoprim-sulfamethoxazole, while Salmonella showed complete susceptibility to all tested antibiotics. The observed resistance patterns correlated with antimicrobial usage estimated from sales data from a large-scale wholesale and retail company. Six (6/14, 42.9%) E. coli isolates tested positive for blaCTX-M, whilst eight (8/14, 57.1%) Enterobacter samples tested positive for blaTEM. Interestingly, four (4/6, 66.7%) of the E. coli isolates carrying blaCTX-M-positive strains were also positive for blaTEM. Sanger sequencing of the PCR products revealed that five (5/6, 83.3%) of the abovementioned isolates possessed the blaCTX-M-15 allele. The results suggest the presence of potentially pathogenic ESBL-producing Enterobacteriaceae in poultry, threatening public health.

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Section: Discussionsupporting

confidence: 79%

Detection of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Enterobacteriaceae from Diseased Broiler Chickens in Lusaka District, Zambia

Chileshe,

Shawa,

Phiri

et al. 2024

Antibiotics

View full text Add to dashboard Cite

show abstract

“…In our study, amplicon sequencing of blaCTX-M revealed the predominance of blaCTX-M-15 (5/6, 83%) among the CTX-resistant E. coli isolates. These findings are in line with the fact that blaCTX-M-15 is the most widely spread ESBL genotype globally [32]. The emergence of blaCTX-M-15 has been attributed to the clonal spread of the E. coli O25b:H4-ST131 pandemic clone.…”

Section: Discussionsupporting

confidence: 79%

Detection of Extended-Spectrum Beta-Lactamase (ESBL) Producing Enterobacteriaceae from Diseased Broiler Chickens in Lusaka District, Zambia

Chileshe,

Shawa,

Phiri

et al. 2023

Preprint

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: Poultry products in Zambia form an integral part of the human diet in many households, as they are cheap and easy to produce. The burden of poultry diseases has, however, remained a major challenge. Growing consumer demand for poultry products in Zambia has resulted in non-prudent antimicrobial use on farms, intending to prevent and treat poultry diseases for growth optimization and maximising profits. This cross-sectional study aimed to identify the different types of bacteria causing diseases in chickens in Lusaka and to detect the extended-spectrum-lactamase (ESBL)-encoding genes. We collected 215 samples from 91 diseased chickens at three post-mortem facilities and screened them for gram-negative bacteria. Of these samples, 103 tested positive for various clinically relevant Enterobacteriaceae, including Enterobacter (43/103, 41.7%), Escherichia coli (20/103, 19.4%), Salmonella (10/103, 9.7%), and Shigella (8/103, 7.8%). Other isolated bacteria included Yersinia, Morganella, Proteus, and Klebsiella, which accounted for 21.4%. E. coli, Enterobacter, Salmonella, and Shigella were subjected to antimicrobial susceptibility testing. The results revealed that E. coli, Enterobacter, and shigella were highly resistant to tetracycline, ampicillin, amoxicillin, and trimethoprim-sulfamethoxazole, while Salmonella showed complete susceptibility to all antibiotics. The observed resistance patterns correlated with antimicrobial usage estimated from sales data from a large-scale wholesale and retail company. Six (6/14, 42.9%) E. coli isolates tested positive for blaCTX-M, whilst eight (8/14, 57.1%) Enterobacter samples tested positive for blaTEM. Interestingly, four (4/6, 66.7%) of the E. coli isolates carrying blaCTX-M-positive strains were also positive for blaTEM. Sanger sequencing of the PCR products revealed that five (5/6,83.3%) of the abovementioned isolates possessed the blaCTX-M-15 allele. The results suggest the presence of potentially pathogenic ESBL-producing Enterobacteriaceae in poultry, threatening public health. Keywords:. Enterobacteriaceae, blaCTX-M, blaTEM

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Extended-spectrum beta-lactamase genes distribution in Pseudomonas species from livestock samples in Ibadan, Nigeria

et al. 2020

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TEM Gene Detection in Clinical Pseudomonas aeruginosa and Escherichia coli Samples

Abstract: .

Cited by 3 publications

References 7 publications

Detection of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Enterobacteriaceae from Diseased Broiler Chickens in Lusaka District, Zambia

Detection of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Enterobacteriaceae from Diseased Broiler Chickens in Lusaka District, Zambia

Detection of Extended-Spectrum Beta-Lactamase (ESBL) Producing Enterobacteriaceae from Diseased Broiler Chickens in Lusaka District, Zambia

Extended-spectrum beta-lactamase genes distribution in Pseudomonas species from livestock samples in Ibadan, Nigeria

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