Telomeres et cotelomeres d'interet biologique et biomedical—IV. Les telomeres du tris(hydroxymethyl)-acrylamidomethane nouveaux agents amphiphiles non ioniques

Pucci, Bernard; Maurizis, Jean‐Claude; Pavia, A. A.

doi:10.1016/0014-3057(91)90087-5

Cited by 47 publications

(28 citation statements)

References 7 publications

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“…Fluorinated surfactants are remarkably stable. This kind of molecule can lower the surface tension of water more than hydrocarbon-based analogues, but they do not exhibit any detergent power (Pucci et al, 1991). They have several advantages : because of their low MW (molecular weight), they should be able to cross biological membranes and no accumulation phenomenon in the organism should be noted (Maurizis et al, 1994;Chehade et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

An in vitro study of the sub‐cellular distribution of nicotinic receptors

Devillers‐Thiéry

Bourgeois

Pons

et al. 2003

Biology of the Cell

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Nicotinic and serotoninergic 5HT3 receptors share important sequence identities except for their cytoplasmic loop. Both ends of this loop display conserved 3D helical structures with distinct primary sequences. We decided to check whether these two helices named F and G play a role in the sub-cellular distribution of different nicotinic receptors. We systematically exchanged each helix with the equivalent sequence of neuronal nicotinic and a4, b2 and a7 subunits in the functional chimeric a7-5HT3 receptor used as a model system. The new chimeras were expressed in vitro in polarized epithelial cells from pig kidney. We quantified synthesis and export of the receptors to the cell surface by measuring a-bungarotoxin binding sites. Immunogold labelling was used, at the electron microscope level, to determine the amount of each chimera present at either domain, apical and/or basolateral, of these cells. We noticed that in epithelial cells the majority of a-bungarotoxin binding sites remained sequestered in the cytoplasm as already observed in neurons in vivo. The majority of the pentamers present at the cell surface were located at the apical domain. Our results suggest that helix F and G differently regulate assembly and export to the cell surface of a-bungarotoxin binding receptors.

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Section: Discussionmentioning

confidence: 99%

An in vitro study of the sub‐cellular distribution of nicotinic receptors

Devillers‐Thiéry

Bourgeois

Pons

et al. 2003

Biology of the Cell

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“…1), the synthesis of which was described in previous papers. 30,31 We followed the published protocol as summarized below. Surfactants were obtained by free radical polymerization of an acrylamide monomer derived from Tris, the tris(hydroxymethyl) acrylamidomethane (THAM), or from its peracetylated analogue (tris(acetoxymethyl)acrylamidomethane) in the presence of a peruoroalkanethiol as a transfer reagent called "telogen".…”

Section: Methodsmentioning

confidence: 99%

“…The physico-chemical parameters of these telomers (molecular weight, hydrophilic lipophilic balance or HLB, critical micelle concentration or CMC) can be adjusted through both the starting material and the experimental conditions. 30,32 Telomerization experiments were respectively performed in methanol (MeOH), when the monomer THAM was used as the starting material, and in tetrahydrofuran (THF) for peracetylated THAM. The later monomer is required to obtain a Degree of Polymerization (DPn) higher than 15 according to Giusti et al 33 due to the observed limited solubility of polyTris oligomers in methanol.…”

Section: Methodsmentioning

confidence: 99%

“…Taking into account published results about THAM telomerization, 18,30 we choose the values 5, 10, 15, 20, and 25 for the monomer over telogen ratio in order to obtain FTAC surfactants with a DPn (average number of repeating units on the polymeric backbone + 1 telogen moiety, Fig. 1) equal to 7, 13, 18, 22, and 27, respectively.…”

Section: Characterization Of Uorinated Surfactantsmentioning

confidence: 99%

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Perfluorocarbon nanodroplets stabilized by fluorinated surfactants: characterization and potentiality as theranostic agents

et al. 2015

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We aim to produce emulsions that can act as contrast agents and drug carriers for cancer imaging and therapy. To increase tumor detection and decrease drug side effects, it is desirable to take advantage of the enhanced permeability and retention effect that allows nanoparticles to accumulate in tumor tissues.To do so, the emulsion droplets need to be small enough and stable over time in addition to enhancing image contrast and carrying a drug payload. In the present study, we have investigated the properties and potentiality as theranostic agents of perfluorocarbon emulsions stabilized by a biocompatible fluorinated surfactant called FTAC. To obtain better control of our system, the synthesis of those surfactants was studied and their physico-chemical properties were explored in different configurations such as micelles, in the perfluorocarbon droplet shell and at water/air and water/perfluorocarbon interfaces. The originality of this work lies in the determination of numerous characteristics of emulsions and fluorinated surfactants including surface tension, interfacial tension, critical micelle concentration, adiabatic compressibility, density, size distribution (aging studies), and ultrasonic echogenicity. These characterization studies were conducted using different types of FTAC and several perfluorocarbons (perfluoropentane, perfluorohexane, and perfluorooctyl bromide). We have also shown that a hydrophobic drug could be encapsulated in the FTAC-stabilized perfluorocarbon droplets thanks to triacetin addition. Finally, the perfluorocarbon emulsions were detectable in vitro by a clinical 3 T MRI scanner, equipped with a double frequency 19 F/ 1 H transmit-receive coil.

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“…The physicochemical parameters of these telomers (molecular weight, hydrophilic-lipophilic balance, electric charge) can be adjusted through both the starting material and the experimental conditions. [29,30] With regard to their biological properties, we previously showed that these multifunctional carriers are able to cross physiological membranes and diffuse through all tissue types. [31] A whole-body autoradiography performed on rat and mouse allowed us to confirm the ubiquitous biodistribution of THAM-derived telomers in all biological compartments except the brain.…”

Section: Introductionmentioning

confidence: 99%

Thalidomide Derivatives for the Treatment of Neuroinflammation

Contino‐Pépin¹,

Parat²,

Patinote³

et al. 2010

ChemMedChem

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The precise mechanism-of-action of thalidomide remains uncertain and might differ between diseases and under different clinical condition. With implications in the treatment of a variety of inflammatory and autoimmune diseases, as well as for use as an anticancer agent, alone or in combination with established therapeutics, it is clear that thalidomide and its derivatives deserve further scrutiny. In particular, thalidomide was shown to be effective in a mouse model of multiple sclerosis (MS), an autoimmune inflammatory disorder, called experimental autoimmune encephalomyelitis (EAE). Herein, we describe the synthesis and preliminary biological evaluation of new macromolecular prodrugs of thalidomide bearing an aminoalkyl group on the phthalimide ring. The effectiveness of these compounds to limit EAE was investigated, and it was shown that, at 100 mg kg⁻¹ thalidomide-equivalent dose, they abrogated the clinical and pathological features of EAE.

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Telomeres et cotelomeres d'interet biologique et biomedical—IV. Les telomeres du tris(hydroxymethyl)-acrylamidomethane nouveaux agents amphiphiles non ioniques

Cited by 47 publications

References 7 publications

An in vitro study of the sub‐cellular distribution of nicotinic receptors

An in vitro study of the sub‐cellular distribution of nicotinic receptors

Perfluorocarbon nanodroplets stabilized by fluorinated surfactants: characterization and potentiality as theranostic agents

Thalidomide Derivatives for the Treatment of Neuroinflammation

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