Telomere shortening does not occur during postmaturational aging in situ in normal human oral fibroblasts

Kang, Mo K.; Kameta, Ayako; Baluda, M A; Park, No-Hee

doi:10.1016/s0047-6374(03)00145-3

Cited by 6 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that telomere length measurements do not show a clear correlation with tissue renewal times in vivo , but rather are characteristic for individuals and are correlated with those in other organs 48 . Therefore, fibroblasts might retain their original telomere length to a greater degree than epithelial cells, supporting the findings for human oral fibroblasts 49 …”

Section: Relative Telomere Lengths Measured By Q‐fish Using Human Tismentioning

confidence: 61%

“…48 Therefore, fibroblasts might retain their original telomere length to a greater degree than epithelial cells, supporting the findings for human oral fibroblasts. 49 The telomere length patterns differed between neonates and adults. Telomeres of fibroblasts were relatively shorter than those of epithelial cells in neonates less than 1 month-old, but were longer than those of epithelial cells in individuals over 1 month-old.…”

Section: Lingual Mucosa and Epithelial Tissue Stem Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Changes of telomere length with aging

Takubo

Aida

Izumiyama-Shimomura

et al. 2010

Geriatrics Gerontology Int

View full text Add to dashboard Cite

We reviewed our methodology and results of telomere measurements, with reference to telomere length and aging. Human tissues always showed telomere shortening with age, except for the brain and myocardium. Yearly rates of telomere length reduction in various tissues were mostly within the range 20-60 bp, and thus compatible with that expected from only one round of mitosis. It was suggested that when telomeres were found to be longer in any specific organ in a given individual, then the other organs in that individual would also have longer telomeres. Using the quantitative fluorescence in situ hybridization (Q-FISH) method for telomere measurement, we were able to measure the telomere lengths of various cell types within tissues. Here we summarize the results obtained for various cell types in the stomach, tongue and breast. Our Q-FISH method using our original software program "Tissue Telo" is excellent for measuring telomere lengths using tissue sections and PNA probes. Geriatr Gerontol Int 2010; 10 (Suppl. 1): S197-S206.

show abstract

Section: Relative Telomere Lengths Measured By Q‐fish Using Human Tismentioning

confidence: 61%

Section: Lingual Mucosa and Epithelial Tissue Stem Cellsmentioning

confidence: 99%

Changes of telomere length with aging

Takubo

Aida

Izumiyama-Shimomura

et al. 2010

Geriatrics Gerontology Int

View full text Add to dashboard Cite

show abstract

“…In some cells such as fibroblasts a telomere does not shorten (18) and long telomere length has been reported in newborns (19), fetuses (20), and zygotes (21). However, the individuals with longer telomere length seem to have higher life ex pectancy.…”

Section: Introductionmentioning

confidence: 99%

Association Between Telomere Shortening and Ageing During Occupational Exposure

Eshkoor¹,

Jahanshiri²,

Ismail³

et al. 2012

Journal of Medical Biochemistry

View full text Add to dashboard Cite

Summary: Telomere length is considered as a biomarker of ageing, resulting in shortening during the process. The present investigation was an attempt to determine the relative telomere length in mechanical workshop workers. Telomere length shortening in cells during occupational expo sure causes accelerated ageing. Genomic DNA was isolated from buccal epithelial cells collected from 240 individuals, comprising two groups of 120 exposed workers and 120 unexposed controls. Telomere length was measured by using real time PCR. Both telomere (T) and single copy gene (S) specific primers were used to compute the relative T/S ratio and expressed as the relative telomere length. Telomere length differed significantly between the workers and controls (p<0.05). The results showed an indirect and significant association (r=-0.356, p=0.001) between age and telomere length in the workers. This study showed that the difference in telomere length shortening was statistically significant (p<0.05) between the workers and controls. It was concluded that occupational exposure acts as a risk factor to enhance telomere length shortening and accelerate ageing.Keywords: telomere, 36B4 gene, ageing, occupational exposure, buccal cells, real-time PCR Kratak sadr`aj: Du`ina telomera smatra se biomarkerom starenja i tokom ovog procesa rezultira skra}enjem. Ovo istra`ivanje predstavlja poku{aj odre|ivanja relativne du`ine telomera kod radnika u mehani~arskim radionicama. Skra}enje du`ine telomera u }elijama tokom profesionalne izloenosti izaziva ubrzano starenje. Genomska DNK izolovana je iz epitelnih }elija unutra{nje strane obraza sakupljenih od 240 osoba, koji su ~inili dve grupe: 120 profesionalno izloenih radnika i 120 neizlo`enih kontrolnih subjekata. Du`ina telomera izmerena je tehnikom PCR u realnom vremenu. Specifi~ni prajmeri telomera (T) i gena prisutnih u jednoj kopiji (S) upotrebljeni su za izra~unavanje relativne razmere T/S i izra`eni kao relativna du`ina telomera. Du`ina telomera zna~ajno se razlikovala izme|u radnika i kontrolnih subjekata (p<0,05). Rezultati su pokazali da izme|u starosti i du`ine telomera kod radnika postoji indirektna i zna~ajna pove zanost (r=-0,356, p=0,001). Ova studija je pokazala da je razlika u skra}enju du`ine telomera izme|u radnika i kontrolnih subjekata bila statisti~ki zna~ajna (p<0,05). Zaklju~eno je da profesionalna izlo`enost predstavlja faktor rizika za znatnije skra}enje du`ine telomera i ubrzano starenje.Klju~ne re~i: telomera, gen 36B4, starenje, profesionalna izlo`enost, }elije unutra{nje strane obraza, PCR u realnom vremenu

show abstract

Telomere Shortening and Alzheimer’s Disease

2012

View full text Add to dashboard Cite

Telomeres, at the ends of chromosomes and strands of genetic material, become shorter as cells divide in the process of aging. Telomere length has been considered as a biological marker of age. Telomere length shortening has also been evidenced as the causable role in age-related neurodegenerative diseases, including Alzheimer's disease (AD). It has been demonstrated that telomere shortening has been associated with cognitive impairment, amyloid pathology and hyper-phosphorylation of tau in AD and plays an important role in the pathogenesis of AD via the mechanism of oxidative stress and inflammation. However, it seems that there is no relationship between telomere shortening and AD. Therefore, it is essential for further clarification of telomere-related pathogenesis in AD.

show abstract

Telomere shortening does not occur during postmaturational aging in situ in normal human oral fibroblasts

Cited by 6 publications

References 11 publications

Changes of telomere length with aging

Changes of telomere length with aging

Association Between Telomere Shortening and Ageing During Occupational Exposure

Telomere Shortening and Alzheimer’s Disease

Contact Info

Product

Resources

About