Telomere length and human telomerase reverse transcriptase (hTERT) level in patients with acute myeloid leukemia: Impact on clinical outcome

Belbesy, Samia F. El; Aggan, Hanaa A. El; Sultan, Hala K.; Naggar, Amel A. El; Ahmed, Hamdino M.I.

doi:10.1016/j.achaem.2015.07.003

Cited by 4 publications

(6 citation statements)

References 28 publications

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“…Furthermore, our study results are not only in accordance with those of several other groups who have identified telomerase activity in the majority of malignant tumors, but also in line with those who have identified telomerase activity in proliferative diseases, predisposing the development of malignant tumors [28][29][30][31][32] .…”

Section: Discussionsupporting

confidence: 92%

Telomerase activity associated with progression of cervical lesions in a group of Colombian patients

Castro-Duque¹,

Loango-Chamorro²,

Ruiz-Hoyos³

et al. 2015

Rev. Bras. Ginecol. Obstet.

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PURPOSE To analyze the relation between the cytological findings and telomerase activity (TA). METHODS Cervical samples were evaluated and classified according to the Bethesda System. Telomerase activity was measured total product generated values (TPG) using the TRAP assay (telomeric repeat amplification protocol); data were analyzed statistically using the χ2 test, with the level of significance set at p<0.05. RESULTS The study was conducted on 102 patients. Of these, 3.9% showed normal cytological findings, 8.8% showed cervicitis; 2% showed Atypical Squamous Cells of Undetermined Significance (ASCUS); 67.6% showed Low Grade Squamous Intraepithelial Lesion (LSIL); 11.8% showed High Grade Squamous Intraepithelial Lesion (H-SIL) and 5.9% showed Squamous Carcinoma. Among telomerase-positive samples, the TPG values were cervicitis

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Section: Discussionsupporting

confidence: 92%

Telomerase activity associated with progression of cervical lesions in a group of Colombian patients

Castro-Duque¹,

Loango-Chamorro²,

Ruiz-Hoyos³

et al. 2015

Rev. Bras. Ginecol. Obstet.

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“…The Area under the curve (AUC) = 0.760 (overall accuracy = 76.0%). This finding indicates that, in AML patients with a value less or equal to 2.01 anm hTERT level, 76.0% of patients will achieve complete remission (sensitivity [true positive cases] was 69.2%, and specificity [true negative cases] was 66.7%) (El Belbesy et al, 2015).…”

Section: Discussionmentioning

confidence: 90%

The Expression of Human Telomerase Reverse Transcriptase in Adult Acute Myeloid Leukemia and Its Correlation With Various Clinico-Pathological Parameters

AlJobori¹,

Jaafar²

2019

GJHS

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BACKGROUND: Acute myeloid leukemia (AML) is a heterogeneous disorder characterized by clonal expansion of myeloid progenitors (blasts) in the bone marrow and peripheral blood, AML accounts for 80% of acute leukemia in adults, its incidence increase with age. AML can be a fatal disease so research to predict prognosis is important. Telomerase (TA) is an enzyme that stabilizes the telomere length and makes the cell immortal. It is present in some of the normal cells, fetal cells, adult germ cells, and presents in 85% of tumors in humans, it has been shown that TA can be used as a prognostic marker in some solid and hematological neoplasms. Telomere length is a factor that predicts telomere function. AIM: We test the quantitative amount of human telomerase reverse transcriptase (hTERT) gene expression in AML (diagnosed according to FAB) adult and its correlation with various clinic-pathological parameters. PATIENTS & METHODS: We used the TRAP assay to assess the hTERT gene expression in mononuclear blood cells from 40 newly diagnosed AML patients, 25 AML patients after completing their course of treatment, and 15 control health subjects. RESULTS: The mean value of hTERT in AML and control groups were [1.59 ± 1.27 anm and 0.035 ± 0.046 anm respectively], and this difference was significantly higher in patients than in control group (p = 0.0001). The telomerase activity was positive in 27 (67.5%) AML patients, while 13 (32.5%) AML patients were negative for telomerase activity. Twenty-five patients after induction chemotherapy were followed up by bone marrow and peripheral blood examination to determine the patient’s response to therapy. Complete hematological remission was achieved in 12 (48.0%) patients and incomplete hematological remission in 13 (52.0%) patients (14%). The hTERT level was significantly higher in patients before induction chemotherapy than after completion of the induction course (p = 0.0001). The hTERT level at diagnosis in patients who did not achieve complete hematological remission was significantly higher than that in patients who achieved complete hematological remission (p = = 0.026). The hTERT level after induction therapy was significantly higher in patients who did not achieve complete hematological remission than in patients who achieved complete hematological remission (p = 0.003). CONCLUSION: Our research suggests that the hTERT expression could serve as a prognostic marker for AML patients. 

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“…Este problema de "replicação das extremidades" resulta em telômeros mais curtos a cada ciclo de replicação do DNA, e que podem atingir um comprimento criticamente curto. Por conseguinte, esses telômeros curtos podem induzir vias de sinalização na célula (como p53 e p21), desencadeando a parada do ciclo celular, senescência e/ou apoptose (EL BELBESY et al, 2015).…”

Section: Introductionunclassified

“…AALBERS et al, 2013;EL BELBESY et al, 2015).Os telômeros são significativamente mais curtos nas células leucêmicas com citogenética anormal quando comparados àquelas sem anormalidade, sendo que os pacientes com cariótipos com múltiplas aberrações têm os telômeros mais curtos(CAPRARO et al, 2011). Além da citogenética, comprimento telomérico encurtado e aumento da atividade da telomerase também se correlacionam com a progressão da doença e recaída(WANG et al, 2014).…”

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Expressão do RNA telomérico não-codificante TERRA em leucemias agudas e telomeropatias

Zanelatto¹

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Telomere length and human telomerase reverse transcriptase (hTERT) level in patients with acute myeloid leukemia: Impact on clinical outcome

Cited by 4 publications

References 28 publications

Telomerase activity associated with progression of cervical lesions in a group of Colombian patients

Telomerase activity associated with progression of cervical lesions in a group of Colombian patients

The Expression of Human Telomerase Reverse Transcriptase in Adult Acute Myeloid Leukemia and Its Correlation With Various Clinico-Pathological Parameters

Expressão do RNA telomérico não-codificante TERRA em leucemias agudas e telomeropatias

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