Telomere biology of the chicken: A model for aging research

Swanberg, Susan E.; O'Hare, Thomas H.; Robb, Elizabeth A.; Robinson, Charmaine M.; Chang, Hong; Delany, Mary E.

doi:10.1016/j.exger.2010.04.002

Cited by 27 publications

(28 citation statements)

References 78 publications

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“…This was surprising, as the substitution in our case, which resulted in a profound defect in processivity, was a methionine. Moreover, among the species with a methionine at this position, Gallus gallus and Takifugu rubripes have telomerases that are processive in vitro, 40,41 whereas Xenopus laevis telomerase is known to be nonprocessive, producing at most 2 repeats when analyzed by conventional assays. 42 No reports of telomerase activity and processivity in Coturnix japonica were found.…”

Section: Blood 2 May 2013 X Volume 121 Number 18 Reduced Telomerasementioning

confidence: 99%

A homozygous telomerase T-motif variant resulting in markedly reduced repeat addition processivity in siblings with Hoyeraal Hreidarsson syndrome

Gramatges

Sasa

et al. 2013

Blood

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Section: Blood 2 May 2013 X Volume 121 Number 18 Reduced Telomerasementioning

confidence: 99%

A homozygous telomerase T-motif variant resulting in markedly reduced repeat addition processivity in siblings with Hoyeraal Hreidarsson syndrome

Gramatges

Sasa

et al. 2013

Blood

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“…However, in long-lived mammals, after embryogenesis, telomerase is down-regulated in most somatic tissues, which is thought to have evolved as a tumorsuppressing mechanism (7). The pattern in birds is less well understood, but in some species, like the zebra finch Taeniopygia guttata, the pattern is similar to that in long-lived mammals (8)(9)(10). Progressive telomere shortening has been linked to both normal aging and to various degenerative diseases in a wide range of studies mainly in humans and mice (2), and telomere lengthening has been shown to have a restorative effect (11).…”

mentioning

confidence: 99%

Telomere length in early life predicts lifespan

Heidinger

Blount

Boner

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

617

720

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The attrition of telomeres, the ends of eukaryote chromosomes, is thought to play an important role in cell deterioration with advancing age. The observed variation in telomere length among individuals of the same age is therefore thought to be related to variation in potential longevity. Studies of this relationship are hampered by the time scale over which individuals need to be followed, particularly in long-lived species where lifespan variation is greatest. So far, data are based either on simple comparisons of telomere length among different age classes or on individuals whose telomere length is measured at most twice and whose subsequent survival is monitored for only a short proportion of the typical lifespan. Both approaches are subject to bias. Key studies, in which telomere length is tracked from early in life, and actual lifespan recorded, have been lacking. We measured telomere length in zebra finches (n = 99) from the nestling stage and at various points thereafter, and recorded their natural lifespan (which varied from less than 1 to almost 9 y). We found telomere length at 25 d to be a very strong predictor of realized lifespan (P < 0.001); those individuals living longest had relatively long telomeres at all points at which they were measured. Reproduction increased adult telomere loss, but this effect appeared transient and did not influence survival. Our results provide the strongest evidence available of the relationship between telomere length and lifespan and emphasize the importance of understanding factors that determine early life telomere length.aging | Taeniopygia guttata | senescence | bird

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“…The attributes of the chicken provide an additional vertebrate model for investigation of the ALT pathway. Copyright © 2011 S. Karger AG, Basel The telomeres of the chicken genome are maintained by telomerase similar to other vertebrates Swanberg and Delany, 2006;Swanberg et al, 2010]. Telomere maintenance, i.e.…”

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confidence: 99%

Molecular and Cellular Evidence for the Alternative Lengthening of Telomeres (ALT) Mechanism in Chicken

O'Hare

Delany

2011

Cytogenet Genome Res

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Telomere maintenance is an important genetic mechanism controlling cellular proliferation. Normally, telomeres are maintained by telomerase which is downregulated upon cellular differentiation in most somatic cell lineages. Telomerase activity is upregulated in immortalized cells and cancers to support an infinite lifespan and uncontrolled cell growth; however, some immortalized and transformed cells lack telomerase activity. Telomerase-negative tumors and immortalized cells utilize an alternative mechanism for maintaining telomeres termed alternative lengthening of telomeres (ALT). This research explored evidence for the ALT pathway in chicken cell lines by studying nontransformed immortalized cell lines (DF-1 and OU2) and comparing them to a normal (mortal) cell line and a transformed cell line (DT40). The research consisted of molecular and cellular analyses including profiling of telomeric DNA (array sizing and total content), telomerase activity, and expression of genes involved in the telomerase, recombination, and ALT pathways. In addition, an immunofluorescence analysis for an ALT marker, i.e. ALT-associated promyelocytic leukemia bodies (APBs), was conducted. Evidence for ALT was observed in the telomerase-negative immortalized cell lines. Additionally, the APB marker was also found in the other cell systems. The attributes of the chicken provide an additional vertebrate model for investigation of the ALT pathway.

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Telomere biology of the chicken: A model for aging research

Cited by 27 publications

References 78 publications

A homozygous telomerase T-motif variant resulting in markedly reduced repeat addition processivity in siblings with Hoyeraal Hreidarsson syndrome

A homozygous telomerase T-motif variant resulting in markedly reduced repeat addition processivity in siblings with Hoyeraal Hreidarsson syndrome

Telomere length in early life predicts lifespan

Molecular and Cellular Evidence for the Alternative Lengthening of Telomeres (ALT) Mechanism in Chicken

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