Telomere aggregate formation in placenta specimens of pregnancies complicated with pre-eclampsia

Sukenik-Halevy, Rivka; Fejgin, Moshe D.; Kidron, Devora; Goldberg‐Bittman, Lilach; Sharony, Reuven; Biron‐Shental, Tal; Kitay-Cohen, Yona; Amiel, Aliza

doi:10.1016/j.cancergencyto.2009.03.015

Cited by 30 publications

(29 citation statements)

References 22 publications

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“…[88][89][90] These BFB cycles contribute to deletions, gene amplification, and nonreciprocal translocations. 57 Deficiency in dietary methyl donor folate induces NPB formation 60 and is consistent with observations that loss of DNA methylation results in increased telomeric recombination. 91 Though substantial progress has been made in understanding the molecular mechanisms behind NPB formation, 61 the influence of folate deficiency still requires definition.…”

Section: The Breakage-fusion-bridge Cyclesupporting

confidence: 83%

“…Chromatids that lack telomere sequence after dicentric chromosome breakage that is likely fuse again following DNA replication during the S‐phase, forming a dicentric chromosome and further perpetuating the BFB cycle 88–90 . These BFB cycles contribute to deletions, gene amplification, and nonreciprocal translocations 57 . Deficiency in dietary methyl donor folate induces NPB formation 60 and is consistent with observations that loss of DNA methylation results in increased telomeric recombination 91 .…”

Section: The Breakage–fusion–bridge Cyclesupporting

confidence: 77%

“…Telomere dysfunction within a cell has been measured as telomere end‐to‐end associations, telomere aggregates, telomere doublets, nucleoplasmic bridges (NPB), telomere clusters, or telomeric chromatid concatenates, and these have been associated with disease states or predisease conditions. Recent evidence is emerging to show that such telomeric dysfunction is increased under conditions of tumourigenesis and oxidative stress 56,57 . Telomere aggregates can be visualized using 2‐D fluorescence microscopy and may represent telomere‐end fusions that later could potentially become involved in breakage–fusion–bridge (BFB) cycles 58 .…”

Section: The Shelterin Complex and Chromosomal Stabilitymentioning

confidence: 99%

“…Recent evidence is emerging to show that such telomeric dysfunction is increased under conditions of tumourigenesis and oxidative stress. 56,57 Telomere aggregates can be visualized using 2-D fluorescence microscopy and may represent telomere-end fusions that later could potentially become involved in breakage-fusion-bridge (BFB) cycles. 58 Folate deficiency has been shown to induce NPB formation; 59,60 however, it is not clear whether these events are caused by telomere end fusion, misrepair of DNA breaks in telomeres or anywhere else along the chromosome.…”

Section: The Shelterin Complex and Chromosomal Stabilitymentioning

confidence: 99%

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Telomere dynamics: the influence of folate and DNA methylation

Moores

Fenech

O’Callaghan

2011

Annals of the New York Academy of Sciences

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Since the suggestion of their existence, a wealth of literature on telomere biology has emerged aimed at solving the DNA end-underreplication problem identified by Olovnikov in 1971. Telomere shortening/dysfunction is now recognized as increasing degenerative disease risk. Recent studies have suggested that both dietary patterns and individual micronutrients-including folate-can influence telomere length and function. Folate is an important dietary vitamin required for DNA synthesis, repair, and one-carbon metabolism within the cell. However, the potential mechanisms by which folate deficiency directly or indirectly affects telomere biology has not yet been reviewed comprehensively. The present review summarizes recent published knowledge and identifies the residual knowledge gaps. Specifically, this review addresses whether it is plausible that folate deficiency may (1) cause accelerated telomere shortening, (2) intrinsically affect telomere function, and/or (3) cause increased telomere-end fusions and subsequent breakage-fusion-bridge cycles in the cell.

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Section: The Breakage-fusion-bridge Cyclesupporting

confidence: 83%

Section: The Breakage–fusion–bridge Cyclesupporting

confidence: 77%

Section: The Shelterin Complex and Chromosomal Stabilitymentioning

confidence: 99%

Section: The Shelterin Complex and Chromosomal Stabilitymentioning

confidence: 99%

See 2 more Smart Citations

Telomere dynamics: the influence of folate and DNA methylation

Moores

Fenech

O’Callaghan

2011

Annals of the New York Academy of Sciences

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“…Previous studies of placental TL have utilized DNA extracted from an array of sites, with inconsistent attention to confounding maternal tissue, inclusion of multiple cell tissue types, or failing to define the specific sampling site altogether. 28–31,34,35 Given the complexity of placenta these methodological variations curtail their generalizability, warranting further investigation.…”

Section: Introductionmentioning

confidence: 99%

Differences in placental telomere length suggest a link between racial disparities in birth outcomes and cellular aging

Jones

Gambala

Esteves

et al. 2017

American Journal of Obstetrics and Gynecology

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BACKGROUND Health disparities begin early in life and persist across the life course. Despite current efforts Black women exhibit greater risk for pregnancy complications and negative perinatal outcomes compared to White women. The placenta, a complex multi-tissue organ, serves as the primary transducer of bidirectional information between the mother and fetus. Altered placental function is linked to multiple racially disparate pregnancy complications, however little is known about racial differences in molecular factors within the placenta. Several pregnancy complications, including preeclampsia and fetal growth restriction, exhibit racial disparities and are associated with shorter placental telomere length, an indicator of cellular stress and aging. Cellular senescence and telomere dynamics are linked to the molecular mechanisms associated with the onset of labor and parturition. Further, racial differences in telomere length are found in a range of different peripheral tissues. Together these factors suggest that exploration of racial differences in telomere length of the placenta may provide novel mechanistic insight into racial disparities in birth outcomes. OBJECTIVE This study examined whether telomere length measured in four distinct fetally-derived tissues were significantly different between Blacks and Whites. The study had two hypotheses: (1) that telomere length measured in different placental tissue types would be correlated and (2) that across all sampled tissues telomere length would differ by race. STUDY DESIGN In a prospective study, placental tissue samples were collected from the amnion, chorion, villus, and umbilical cord from Black and White singleton pregnancies (N=46). Telomere length was determined using monochrome multiplex quantitative real-time polymerase chain reaction in each placental tissue. Demographic and pregnancy-related data were also collected. Descriptive statistics characterized the sample overall and among Black and White women separately. The overall impact of race was assessed by multilevel mixed-effects linear regression models that included empirically relevant covariates. RESULTS Telomere length was significantly correlated across all placental tissues. Pairwise analyses of placental tissue telomere length revealed significantly longer telomere length in the amnion compared to the chorion (t=−2.06, p=0.043). Overall telomere length measured in placenta samples from Black mothers were significantly shorter than those from White mothers (β=−0.09, p=0.04). Controlling for relevant maternal and infant characteristics strengthened the significance of the observed racial differences (β=−0.12, p=0.02). Within tissue analyses revealed that the greatest difference by race was found in chorionic telomere length (t=−2.81, p=0.007). CONCLUSION These findings provide the first evidence of racial differences in placental telomere length. Telomere length was significantly shorter in placental samples derived from Black mothers compared to White. Given previous studies repo...

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