Telomerase, p53 and Human Papillomavirus Infection in the Uterine Cervix

Nair, Pradip

doi:10.1080/028418600430996

Cited by 25 publications

(25 citation statements)

References 14 publications

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“…In contrast to the findings of Zheng et al [22], no correlation between increased telomerase activity and high-risk HPV was found in this study. Activation of telomerase did not appear to be associated with high-risk HPV, as suggested by Nair et al [23]. Thus, the telomerase assay is not useful in improving the detection of high-grade CIN in cervical screening.…”

Section: Discussionmentioning

confidence: 59%

Telomerase Assay and HPV 16/18 Typing as Adjunct to Conventional Cytological Cervical Cancer Screening

Ngan¹,

Cheung²,

Liu³

et al. 2002

Tumor Biol

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Objective: This study aims at exploring the potential use of telomerase activity assay and typing of human papillomaviruses (HPV) 16 and 18 in improving the identification of high-grade cervical intraepithelial neoplasia (CIN). Methods: From 86 women with normal cervical smears and from 114 patients with abnormal cervical smears cervical scrapings were collected. The telomerase activity was assayed using the Telomerase Repeat Amplification Protocol, and HPV was detected using consensus primers and specific primers for HPV 16 and HPV 18. Results: HPV 16 in cervical scrapes was significantly associated with high-grade squamous epithelial lesions on cytology and with high-grade CIN, i.e., CIN 2/3 on biopsy. The detection of HPV 18 or telomerase activity had no significant association with high-grade squamous intraepithelial lesions or high-grade CIN. Conclusion: The use of the telomerase activity assay in cervical scrapes, unlike HPV 16 typing, did not improve the detection of high-grade CIN.

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Section: Discussionmentioning

confidence: 59%

Telomerase Assay and HPV 16/18 Typing as Adjunct to Conventional Cytological Cervical Cancer Screening

Ngan¹,

Cheung²,

Liu³

et al. 2002

Tumor Biol

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“…Previous studies also reported mixed results, with some studies indicating an association with HPV positivity and others not reporting a correlation [12,15,17,20,21,[24][25][26]31,32]. We previously demonstrated that exogenous expression of HPV type 16 E6 could activate telomerase [33].…”

Section: Discussionmentioning

confidence: 82%

“…Telomerase activity is widely detected in human cancers, whereas nonmalignant somatic tissue exhibits little to no telomerase activity [11]. For cervical cancer, a number of studies have demonstrated that telomerase activity can be detected in biopsy specimens from high-grade lesions at a frequency of >90%, but this occurs at much lower levels and frequency in biopsy specimens from lower-grade lesions [12][13][14][15][16][17][18][19][20][21][22][23]. There is evidence that biopsy specimens that are positive for high-risk HPV types are more likely to contain telomerase activity, although this parameter is difficult to separate from disease grade because nearly all high-grade lesions are positive for HPV.…”

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confidence: 99%

Telomerase Activity as a Potential Diagnostic Marker for Triage of Abnormal Pap Smears

Ault

Allen

Phillips

et al. 2005

Journal of Lower Genital Tract Disease

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Objectives-To determine whether there is an association between high levels of telomerase and premalignant cervical disease and to provide a preliminary analysis of telomerase activity as a potential triage strategy.Materials and Methods-Premenopausal women were invited to participate in the study during routine gynecologic visits as well as visits where colposcopy was performed. Samples were taken from the cervix using a broom device and placed in cold phosphate-buffered saline. A total of 92 samples were evaluated. Cells were counted and lysed, and a semi-quantitative measure of telomerase activity was determined using a commercially available telomerase enzyme-linked immunosorbent assay kit. The presence of human papillomavirus (HPV) types 16 and 18 was assessed by polymerase chain reaction analysis. One-way analysis of variance was used to test for the association of telomerase activity with cytology, HPV type 16 or 18 status, and colposcopy and/or biopsy findings.Results-When telomerase levels were analyzed according to Pap smear results, there were no differences among four groups of cytology findings (normal, atypical squamous cells of undetermined significance, low-grade squamous intraepithelial lesion, and high-grade squamous intraepithelial lesion). When colposcopy and/or biopsy results were considered, significantly higher levels of telomerase were detected in cervical intraepithelial neoplasia (CIN) 2,3 samples than in normal Pap smear samples and CIN 1 samples (p = .035). There was no significant difference in telomerase levels between samples that tested positive for HPV type 16 or 18 and those that did not (p = .111).Conclusions-Telomerase levels were significantly higher in cytologic samples from women with biopsy-proven CIN 2,3 than in samples from women with normal cytology results or CIN 1. These results warrant larger studies to determine whether telomerase activity may be a useful triage tool for abnormal cytologic findings. [5,6]. However, HPV is also widely present in patients without clinically significant disease, thus limiting its use as an indicator of cancer. KeywordsTelomeres are at the ends of human chromosomes and consist of the repeated base pair sequence TTAGGG [7,8]. With each cell division, the telomeres decrease in length until the process results in a signal that triggers senescence [9]. Telomerase is an enzyme that adds a repeated TTAGGG sequence to the telomeres [10]. This prevents senescence by maintaining chromosomal length and function. Telomerase activity is widely detected in human cancers, whereas nonmalignant somatic tissue exhibits little to no telomerase activity [11]. For cervical cancer, a number of studies have demonstrated that telomerase activity can be detected in biopsy specimens from high-grade lesions at a frequency of >90%, but this occurs at much lower levels and frequency in biopsy specimens from lower-grade lesions [12][13][14][15][16][17][18][19][20][21][22][23]. There is evidence that biopsy specimens that are positive for high-risk HPV type...

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“…In this study association between p53 over-expression and HPV infection was noted (Table 3), confirming the inactivation of p53 protein by complex formation with E6 oncoprotein and subsequent stabilization. Altered expression and inactivation of p53 protein in HPV infected cases is well associated with disease progression (Singh et al, 2009;Oh et al, 2004) and reflects the presence of HPV in cervical cells (Scheffner et al, 1990;Nair et al, 2000), thus, could be a useful parameter for clinical interpretations of HPV presence in low resource hospital settings. Along with these, in some of the HPV negative cases also p53 over-expression was noted in our study (Table 3); this might be evoked via pathways other than HPV infection through gene mutation, hypomethylation or hypermethylation, which suggested that some of the observed cervical preneoplastic and neoplastic lesions could be induced in a heterogeneous way.…”

Section: Discussionmentioning

confidence: 99%

Expression of P-glycoprotein is Positively Correlated with p53 in Human Papilloma Virus Induced Squamous Intraepithelial Lesions of Uterine Cervix: Poor Prognosis Association

Singh

Singh²,

Mathur³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Telomerase, p53 and Human Papillomavirus Infection in the Uterine Cervix

Cited by 25 publications

References 14 publications

Telomerase Assay and HPV 16/18 Typing as Adjunct to Conventional Cytological Cervical Cancer Screening

Telomerase Assay and HPV 16/18 Typing as Adjunct to Conventional Cytological Cervical Cancer Screening

Telomerase Activity as a Potential Diagnostic Marker for Triage of Abnormal Pap Smears

Expression of P-glycoprotein is Positively Correlated with p53 in Human Papilloma Virus Induced Squamous Intraepithelial Lesions of Uterine Cervix: Poor Prognosis Association

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