Telomerase-Mediated Apoptosis of Chicken Lymphoblastoid Tumor Cell Line by Lanthanum Chloride

Zhang, Ziwei; Wang, Jintao; Li, Jinlong; Xu, Shiwen

doi:10.1007/s12011-011-9027-8

Cited by 9 publications

(6 citation statements)

References 38 publications

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“…Although it has been previously reported that LaCl 3 affects multiple tumor growth based on cell-based assays and animal experiments ( 10 , 12 – 14 ), its precise biological role remains largely unclear. Therefore, further investigations on the underlying mechanisms are required.…”

Section: Discussionmentioning

confidence: 99%

Effect of lanthanum chloride on tumor growth and apoptosis in human ovarian cancer cells and xenograft animal models

et al. 2018

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Ovarian cancer is the leading cause of mortality resulting from gynecologic cancer. A common anti-ovarian tumor drug is cisplatin; however, repeated use of cisplatin causes severe resistance and leads to poor long-term survival rate in ovarian cancer patients. Recently, it was reported that lanthanum chloride (LaCl3) may inhibit tumor growth and induce apoptosis in certain cancer cells. In the present study, the effect of LaCl3 on ovarian cancer was determined in vivo and in vitro. A cisplatin-sensitive human ovarian cancer cell line, COC1, was used in the current study. A xenograft animal model of ovarian cancer was established injecting COC1 or cisplatin-resistant COC1 cells (COC1/DDP) cells into mice. A TUNEL assay was used to determine the apoptosis of the COC1 or COC1/DDP cells and a immunohistochemical assay was conducted to measure the expression of B-cell lymphoma-2, Ki67, breast cancer 1 (BRCA)1, BRCA2 and excision repair cross-complementation group 1 in COC1 or COC1/DDP cells. It was observed that LaCl3 promoted apoptosis in COC1 and COC1/DDP cells. In addition, LaCl3 plus cisplatin led to further increase in the expression levels of tumor suppressor genes and decrease in the expression of oncogenes. Furthermore, application of LaCl3 and cisplatin inhibited tumor growth in vivo in a xenograft animal model. These results indicated the synergistic role of LaCl3 on cisplatin-induced inhibition of cancer cell proliferation and tumor growth, providing a potential and effective candidate for the treatment of ovarian tumors.

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Section: Discussionmentioning

confidence: 99%

Effect of lanthanum chloride on tumor growth and apoptosis in human ovarian cancer cells and xenograft animal models

et al. 2018

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“…Cellular production of hydrogen peroxide, according to the concentration, can stimulate either growth or death [56]. Interestingly, low concentrations or short incubation times with lanthanum ion induce ROS formation [19] and stimulate cell proliferation [57], while, on the contrary, longer incubation times and larger concentrations of lanthanum ions inhibit growth and induce apoptosis [57][58][59]. Probably, in the first case, proliferative signalling by ROS is prevailing over proapoptotic conditions generated by massive production of ROS, possibly of mitochondrial origin [19].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of thioredoxin reductase by lanthanum chloride

Citta

Folda

Scutari

et al. 2012

Journal of Inorganic Biochemistry

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“…Telomerase is a ribonucleoprotein enzyme that adds tandem (TTAGGG)n repeats to the ends of chromosomes in most eukaryotes [ 1 ]. The structure of chicken telomerase, which consists of chicken telomerase reverse transcriptase (chTERT), telomerase RNA component and telomerase-associated protein, is similar to that of human telomerase [ 2 , 3 ]. Telomerase activation is considered a key step in cell carcinogenesis, and telomerase is one of the most commonly used molecular markers for a wide range of malignant tumours [ 4 – 6 ].…”

Section: Introductionmentioning

confidence: 99%

Mutual regulation between chicken telomerase reverse transcriptase and the Wnt/β-catenin signalling pathway inhibits apoptosis and promotes the replication of ALV-J in LMH cells

Xiang

et al. 2021

Vet Res

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This study aimed to explore the mutual regulation between chicken telomerase reverse transcriptase (chTERT) and the Wnt/β-catenin signalling pathway and its effects on cell growth and avian leukosis virus subgroup J (ALV-J) replication in LMH cells. First, LMH cells stably overexpressing the chTERT gene (LMH-chTERT cells) and corresponding control cells (LMH-NC cells) were successfully constructed with a lentiviral vector expression system. The results showed that chTERT upregulated the expression of β-catenin, Cyclin D1, TCF4 and c-Myc. chTERT expression level and telomerase activity were increased when cells were treated with LiCl. When the cells were treated with ICG001 or IWP-2, the activity of the Wnt/β-catenin signalling pathway was significantly inhibited, and chTERT expression and telomerase activity were also inhibited. However, when the β-catenin gene was knocked down by small interfering RNA (siRNA), the changes in chTERT expression and telomerase activity were consistent with those in cells treated with ICG001 or IWP-2. These results indicated that chTERT and the Wnt/β-catenin signalling pathway can be mutually regulated. Subsequently, we found that chTERT not only shortened the cell cycle to promote proliferation but also inhibited apoptosis by downregulating the expression of Caspase 3, Caspase 9 and BAX; upregulating BCL-2 and BCL-X expression; and promoting autophagy. Moreover, chTERT significantly enhanced the migration ability of LMH cells, upregulated the protein and mRNA expression of ALV-J and increased the virus titre. ALV-J replication promoted chTERT expression and telomerase activity.

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Telomerase-Mediated Apoptosis of Chicken Lymphoblastoid Tumor Cell Line by Lanthanum Chloride

Cited by 9 publications

References 38 publications

Effect of lanthanum chloride on tumor growth and apoptosis in human ovarian cancer cells and xenograft animal models

Effect of lanthanum chloride on tumor growth and apoptosis in human ovarian cancer cells and xenograft animal models

Inhibition of thioredoxin reductase by lanthanum chloride

Mutual regulation between chicken telomerase reverse transcriptase and the Wnt/β-catenin signalling pathway inhibits apoptosis and promotes the replication of ALV-J in LMH cells

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