Telomerase in relation to clinicopathologic prognostic factors and survival in cervical cancer

Telomerase activity as an adjunct to high-risk human papillomavirus types 16 and 18 and cytology screening in cervical cancer

“…This may be explained due to the upregulation of hTERT before the reactivation of telomerase activity. Similar observations were made in other cancers including cervical cancer by several authors (Kyo et al, 1997;Nakano et al, 1998;Shroyer et al, 1998;Wisman et al, 2001).…”

“…Possible inhibition of the TRAP assay by the presence of normal cervical cells is not going to interfere with sensitivity of the assay since Kyo et al (1997) observed that the detection limit of TRAP assay was 100 cancer cells. Furthermore, Wisman et al (2001) successfully assayed telomerase activity even in 10 cervical cells.…”

“…The average telomerase activity increased with the progression of the clinical stage. The gradual elevation of telomerase activity from mild to moderate to high to very high telomerase activity in different dysplsia and invasive cancer samples indicate that with the progression of the disease there is a concomitant increase in the telomerase activity (Kyo et al, 1997;Shroyer et al, 1998: Wisman et al, 2001Baege et al, 2002).…”

Clinical utility of telomerase in cancer

“…Telomerase activity is a useful indicator to determine malignant grade in the group of tumors in which benign and malignant tumors are sometimes indistinguishable by morphological examination such as brain tumors (Falchetti et al, 1999;Langford et al, 1997), papillary adenocarcinoma in thyroid (Matthews et al, 2001), pancreatic endocrine tumors (Pearson et al, 2000), urothelial cell carcinoma (Lancelin et al, 2000), and cervical intraepithelial neoplasia (Wisman et al, 2001). In these tumors, the levels of telomerase activity subsequently correlated to the prognosis of the patients.…”

Analysis of human serum by liquid chromatography–mass spectrometry: Improved sample preparation and data analysis

“…Various methods have been applied in recent years for the discovery of biomarkers or biomarker patterns of major human diseases, especially for various types of cancer [1][2][3][4][5][6][7][8][9][10][11][12][13]. Amongst these surface enhanced laser desorption ionization mass spectrometry (SELDI-MS), which combines on-chip sample preparation with mass spectrometric analysis, has taken a prominent position [14], although more recent results question the viability of this approach [15,16].…”