Telomerase catalytic subunit in laryngeal carcinogenesis—an immunohistochemical study

Luzar, Boštjan; Poljak, Mario; Gale, Nina

doi:10.1038/modpathol.3800275

Cited by 16 publications

(18 citation statements)

References 21 publications

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“…The patterns of hTERT expression in these frozen sections did not vary essentially from the picture presented on the paraffin sections from HNSCC already examined (47)(48)(49)(50)(51). With an antibody not described in more detail, Nguyen et al (47) demonstrated hTERT in all carcinomas examined and, albeit significantly less, in dysplastic squamous epithelia.…”

Section: Discussionmentioning

confidence: 97%

“…None of the few immunohistochemical studies dealing with hTERT on squamous cell carcinomas in the head and neck has correlated its results to telomerase activity (47)(48)(49)(50)(51). Gonzalez-Quevedo et al (52), who demonstrated hTERT in the RT-PCR in various tumors, pointed to a direct correlation between telomerase activity (determined in TRAP-ELISA) and hTERT.…”

Section: Correlation Of Htert Score Values and Telomerase Activity Inmentioning

confidence: 99%

“…Volpi et al (69) reported nuclear, nucleolar and cytoplasmic proof of hTERT with a monoclonal antibody Tel 366-10 in different cell lines and in normal tissue (skin, prostate, mamma, cervix, bladder, kidney, pancreas and colon). Using the monoclonal NCL-hTERT, Luzar et al (49) found the lowest hTERT expression in normal larynx epithelium from control persons (mean hTERT index 0.17) in comparison to increasing expression in hyperplastic tissue (0.44), in basal hyperplasia (0.54), atypical hyperplasia (0.91), carcinoma in situ (1.05) and in larynx carcinoma (0.96).…”

Section: Correlation Of Htert Score Values and Telomerase Activity Inmentioning

confidence: 99%

“…Luzar et al (49) compared immunohistochemical proof of hTERT detected with the NCL-hTERT antibody (our Ab 3) with RT-PCR results in tumor margin tissues from larynx carcinomas showing varying degrees of hyperplasia. Both methods showed that increasing hTERT expression is an early event in larynx carcinogenesis.…”

Section: Cells Which May Be Responsible For Telomerase Activity Outsimentioning

confidence: 99%

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Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues

Fabricius

Kruse-Boitschenko²,

Khoury³

et al. 2009

Int J Oncol

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Abstract.In previous studies we demonstrated telomerase activity in frozen tissue from head and neck squamous cell carcinoma (HNSCC) and their tumor-free tumor margins. In the present study frozen sections from the same tissues were examined for in situ presence of hTERT. In preliminary investigations we established that the most suitable method of tissue preparation was fixation in acetone and methanol followed by steaming and visualization by APAAP. Most of the assays involved eleven anti-hTERT antibodies and were supplemented with the inclusion of antibodies Ki-67, antinucleolin and CD45. hTERT expression was investigated in the tissues of 61 patients with HNSCC and 37 patients without tumor. Semi-quantitative immunoreactive scores were correlated with telomerase activity. We examined the prognostic significance of hTERT expression with Kaplan-Meier curves and tested the immunological specificity of the antibodies by immunoabsorption with two hTERT peptides and a nucleolin peptide. Nuclear staining of satisfactory distribution and intensity was achieved in seven anti-hTERT antibodies both in the carcinomas and in the squamous epithelia of the tumor resection margins and in the control tissues. Proof of hTERT did not differ from telomerase activity. The telomerase activity demonstrated in tumor-free resection margins and in control tissues did, however, correlate with lymphocytic-monocytic infiltration (CD45 expression). This telomerase activity might be related to nuclear hTERT expression in the squamous epithelium, given that the hTERT score values in the connective tissue tended to be negative. The prognostic significance of hTERT expression demonstrated on paraffin sections from different tumor localizations was not confirmed for the frozen sections of patients with HNSCC. The hTERT specificity of the monoclonal NCL-L-hTERT, whose use as an antibody against hTERT has been questioned, was reexamined with immunohistochemical methods, but the intensity of its immunoabsorption with the nucleolin peptide did not exceed that observed in the other anti-hTERT antibodies.

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Section: Discussionmentioning

confidence: 97%

Section: Correlation Of Htert Score Values and Telomerase Activity Inmentioning

confidence: 99%

Section: Correlation Of Htert Score Values and Telomerase Activity Inmentioning

confidence: 99%

Section: Cells Which May Be Responsible For Telomerase Activity Outsimentioning

confidence: 99%

See 2 more Smart Citations

Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues

Fabricius

Kruse-Boitschenko²,

Khoury³

et al. 2009

Int J Oncol

View full text Add to dashboard Cite

show abstract

“…Using the monoclonal NCL-hTERT (our Ab 3), according to Wu et al (64) an anti-nucleolin antibody, Luzar et al (82) found the lowest hTERT expression in normal larynx epithelium from control persons (mean hTERT index 0.17) in comparison to increasing expression in hyperplastic tissue (mean hTERT index 0.44), in basal hyperplasia (mean hTERT index 0.54) and atypical hyperplasia (mean hTERT index 0.91). Volpi et al (83) reported nuclear, nucleolar and cytoplasmic staining of hTERT with a monoclonal antibody Tel 366-10 in normal tissue from skin (among other tissues).…”

Section: Comparison Of Htert Results In Basal Cell Carcinomas and Squmentioning

confidence: 99%

Localization of telomerase hTERT protein in frozen sections of basal cell carcinomas (BCC) and tumor margin tissues

Fabricius

Kruse-Boitschenko²,

Khoury³

et al. 2009

Int J Oncol

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Abstract.In previous studies we demonstrated telomerase activity in frozen tissues from BCC and their tumor-free margins by the PCR ELISA. In this study we examined in the same frozen sections immunohistochemical presence of hTERT in the nucleus. After fixation in acetone and methanol followed by steaming we used for visualization the antigenantibody reactions by APAAP. This was the best method of preparation of the frozen sections in our preliminary hTERT-study with squamous cell carcinomas. This study was supplemented with antibodies against Ki-67, nucleolin, common leucocyte antigen CD45 and mutated p53. The immunoreactive scores were determined and included the comparison with telomerase activity. The investigation of hTERT expression was performed in the tissues of 41 patients with BCC and control tissues of 14 patients without tumor. Eleven commercial antibodies were used for a nuclear staining of hTERT expression. With the anti-hTERT antibodies we looked for both satisfactory distribution and intensity of immunohistochemical labeling in the carcinomas and in the squamous epithelia of the tumor centers, of the tumor-free margins and of the control tissues. The hTERT expression in the BCC was distributed heterogeneously. The score values established by the anti-hTERT antibodies used were variably or significantly increased. In the stroma they tended to be negative, so we disregarded stroma hTERT. Proof of hTERT did not differ uniformly from telomerase activity. We compared the high with the lower median hTERT values in the Kaplan-Meier curve. Patients with lower hTERT scores in the center or tumor margin as shown by some of the antibodies suffered relapse earlier. Finally, we compared the hTERT expression in BCC tissues with the hTERT scores in HNSCC tissues from our previous study. Only one anti-hTERT antibody (our Ab 7) yielded significantly higher scores in BCC than in HNSCC.

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Molecular Pathology of Head and Neck Tumors

Fisch,

St. John,

Sajed

2023

Molecular Surgical Pathology

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Telomerase catalytic subunit in laryngeal carcinogenesis—an immunohistochemical study

Cited by 16 publications

References 21 publications

Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues

Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues

Localization of telomerase hTERT protein in frozen sections of basal cell carcinomas (BCC) and tumor margin tissues

Molecular Pathology of Head and Neck Tumors

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