Telomerase activity in pleural effusions: diagnostic significance.

Yang, Cheng‐Ta; Lee, Mei-Hsia; Lan, Ray-Shee; Chen, Jan-Kan

doi:10.1200/jco.1998.16.2.567

Cited by 63 publications

(38 citation statements)

References 29 publications

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“…Likewise, the rate of positive telomerase activity in nonmalignant cytological samples has been reported to be y10% in previous reports [9,17,21]. Among the 46 benign samples, a total of nine (19.6%) samples, composed of six with empyema and three with tuberculosis effusions, showed telomerase expression in the present study.…”

Section: Discussionsupporting

confidence: 77%

“…Furthermore, telomerase expression has been shown to be a potential diagnostic biomarker in the fluids of body cavities, however, the present authors are aware of only a few reports investigating the telomerase activity with special reference to pleural effusions [6,17]. These reports showed that the sensitivity of telomerase activity in malignant pleural effusions were 67 and 91%, respectively.…”

Section: Discussionmentioning

confidence: 76%

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Diagnostic implications of telomerase activity in pleural effusions

Dikmen¹,

Di̇kmen²,

Kara³

et al. 2003

Eur Respir J

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The aim of the present study was to investigate the diagnostic efficacy of telomerase activity for discrimination of malignant and benign pleural effusions.Pleural effusions were collected from 109 consecutive patients in whom the diagnosis was confirmed with cytological and/or histological examinations. Cytological samples were classified as malignant (n=63) and benign (n=46). Telomerase activity was determined with the polymerase chain reaction-based telomeric repeat amplification protocol assay.Telomerase activity was detected in 52 (82.5%) and nine (19.6%) samples from the malignant and benign groups, respectively, which was a significant difference. The sensitivity rate of cytological examination when combined with telomerase activity (92.1%) was significantly greater than that of cytological examination alone (53.9%). The sensitivity and specificity of telomerase activity were 82.5 and 80.4%, respectively. Diagnostic accuracy of telomerase activity was 81.6%.Telomerase activity is a highly sensitive diagnostic biomarker for malignancy and may be used as an adjunct to cytological findings in determining malignant pleural effusions. Eur Respir J 2003; 22: 422-426.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 76%

Diagnostic implications of telomerase activity in pleural effusions

Dikmen¹,

Di̇kmen²,

Kara³

et al. 2003

Eur Respir J

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“…47 In bronchial washing cell samples including bronchio-alveolar lavage (BAL) samples, the sensitivity of telomerase is as high as 80% of that in primary cancer tissues resected. [48][49][50] In addition, Yang et al 51 showed more convincing evidence for the clinical utility of telomerase in pleural effusions in three groups: (a) malignant pleural effusions as diagnosed by cytology; (b) nonmalignant pleural effusions; and (c) pleural effusions that were suspected to be malignant, but the findings were inconclusive by cytology. Of 144 samples, telomerase activity was detected in 91% (64 of 70) of malignant samples, 91% (20 of 22) of suspicious samples and only 5.8% (three of 52) of nonmalignant samples.…”

Section: Discussionmentioning

confidence: 99%

Infectivity enhanced, hTERT promoter-based conditionally replicative adenoviruses are useful for SCLC treatment

et al. 2005

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Treatment of advanced small-cell lung cancer (SCLC) remains one of the major challenges in current medicine because of the high morbidity and mortality of the disease. Advanced stage lung cancer is refractory to conventional therapies and it also has an extremely poor prognosis. As a result, new therapeutic approaches are needed. Telomere maintenance to the regulation of replicative lifespan strongly implies that alterations in telomere biology play an important role during malignant transformation. Cancers that exhibit high levels of telomerase activity, such as all of the SCLC, were examined in a previous study. In this study, we turned the expression of human telomerase reverse transcriptase (hTERT) by tumors to a therapeutic advantage using a conditionally replication-competent adenovirus (CRAd) in which the expression of E1 (early region 1) is controlled by the hTERT promoter. This virus achieved good levels of viral replication in SCLC cells and induced a substantial anticancer effect in vitro and in vivo. As a further enhancement, the cancer cell killing effect was improved with a tropism modification of the virus to express the knob domain of Ad3 (serotype 3 adenovirus), and this improved infectivity for cancer cells. Conversely, the hTERT promoter has low activity in normal tissues, and the CRAd caused no damage to normal lung fibroblast cells. Since the telomerase activity is common in many types of cancers, these CRAds may be applicable to a wide range of tumors. We concluded that the use of hTERT promoterbased CRAds may be a potentially effective strategy for cancer treatment.

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“…Our data support the view that, as is the case with exsudates, cytologic and molecular evaluation is warranted in transudates as well. (Yang et al, 1998;Chang et al, 2002;Grünewald et al, 2002). The big advantage of FISH is the direct visualisation of tumour cells exhibiting numeric chromosomal aberrations (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

Sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (FISH)

et al. 2004

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Diagnosis of malignant cells in effusions is important for staging procedures and resulting therapeutic decisions. Cytodiagnostics in effusions is sometimes difficult since reactive mesothelial cells can mimic malignant cells. We used fluorescence in situ hybridisation (FISH) in single-colour or if appropriate in dual-colour evaluation to detect chromosomal aberrations in effusion cells as markers of malignancy, to raise the diagnostic yield. Cytologic and FISH evaluations -by using probes representing several chromosomes always including chromosomes 11 and 17 -were performed in 358 effusion fluids. Cytology was positive for malignancy in 44.4% of all effusions, whereas FISH was positive in 53.9% (P ¼ 0.0001). The combination of cytology and FISH was diagnostic for malignancy in 60.9% of effusions. Diagnostic superiority of FISH was demonstrated in effusions from breast cancer, lung cancer, pancreatic cancer, and in effusions from the entire group of gynaecological and gastrointestinal carcinomas. In transudates (effusion protein o2.5 g dl À1 ), malignant cells were detectable by cytology, FISH, and combined use of both methods in 18.6, 30, and 37.1% of effusions, respectively, suggesting that cytologic and molecular analysis should be performed also with transudates. In conclusion, FISH in combination with conventional cytology is a highly sensitive and specific diagnostic tool for detecting malignant cells in effusions.

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Telomerase activity in pleural effusions: diagnostic significance.

Abstract: Our results indicate that the detection of telomerase activity may be a useful adjunct to cytopathologic methods in the diagnosis of malignant pleural effusions.

Cited by 63 publications

References 29 publications

Diagnostic implications of telomerase activity in pleural effusions

Diagnostic implications of telomerase activity in pleural effusions

Infectivity enhanced, hTERT promoter-based conditionally replicative adenoviruses are useful for SCLC treatment

Sensitive detection of tumour cells in effusions by combining cytology and fluorescence in situ hybridisation (FISH)

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