Telomerase Activity in Diagnosis of Bladder Cancer*

Erdem, Erim; Dikmen, Günnur; Atsü, Necmettin; Doǧan, Pakize; Özen, Haluk

doi:10.1080/00365590310008055

Cited by 11 publications

(4 citation statements)

References 16 publications

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“…For detection of blad der carcinoma in urine specimens, telomerase proved far more sensitive than cytology or other available screens. The sensitivity of telomerase RT-PCR assay ranged from 75.6% to 94%, compared with 34% to 51% for routine cytology and the specificity ranges from 72% to 100% [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

The Diagnostic Potential of Dielectric Properties, Telomerase Activity and Cytokeratin 20 in Urine Cells of Bladder Cancer Patients

Ibrahim¹,

Ghannam²

2012

JCST

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Aim of the study: This work aims to search for mark ers suitable for the screening of bladder cancer, which should be specific, sensitive, reproducible, non-invasive and at acceptable cost. Patients and methods:The study included 45 patients diagnosed as bladder cancer (30 TCC, 15 SCC) of different stages and grades, 20 patients with various urothelial diseases, besides 15 healthy volunteers of matched age and sex to the malignant group.A random midstream urine sample was collected in a sterile container for the determination of telomerase by RT-PCR, keratin 20 by RT-PCR and immunohistochemical staining, urine cytology in addition to DNA dielectric properties.Results: All parameters (telomerase, K20, cytology and DNA dielectric properties) for the malignant group showed significant difference from both the benign and the control groups. With respect to the grade, only K20 showed a significant positive correlation with grade in both TCC and SCC.Conclusion: K20 is the best candidate as screening test for the diagnosis of bladder cancer, representing the highest sensitivity and specificity, beside the radiological and histopathological studies.As a method, RT-PCR is superior to immunostaining for the detection of bladder cancer, meanwhile K20 immunohistochemistry (IHC) results were much better than urine cytology as a bladder cancer screening test. Haematuria and inflammation reduced the specificity of telomerase assay, which reduced its validity as a tumor marker of bladder cancer. The studied DNA has a dielectric dispersion in the frequency range used. There is change in the electric properties of DNA of bladder cancer patients. The dielectric properties of DNA may be used as valuable supplementary markers in diagnosis of bladder cancer.

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Section: Introductionmentioning

confidence: 99%

The Diagnostic Potential of Dielectric Properties, Telomerase Activity and Cytokeratin 20 in Urine Cells of Bladder Cancer Patients

Ibrahim¹,

Ghannam²

2012

JCST

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“…The specificity was increased to 85% for individuals 75 years or younger [34]. A separate study demonstrated similar results with a specificity of 93.5% for the TRAP assay, but a lower sensitivity of 77.4% [35]. For the measurement of the catalytic component of telomerase, hTERT has shown even better results.…”

Section: Telomerasementioning

confidence: 78%

Current and Emerging Bladder Cancer Urinary Biomarkers

Parker

Spiess

2011

The Scientific World JOURNAL

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Bladder cancer continues to be one of the most common malignancies. Those who have been already diagnosed are at high risk for recurrence, especially if the pathology demonstrates high-grade disease. Diagnosis and surveillance is reliant on invasive evaluation with cystoscopy. Urinary cytology has been used to aid in diagnosis, but its use is limited. Other assays have been developed that may aid in clinical decision making. The ultimate goal will be the development of a highly sensitive and specific urinary marker for bladder cancer. This would provide a noninvasive means of diagnosing the disease and limit the number of unnecessary cystoscopies. This article will review the currently available urinary bladder cancer markers. It will also review new and investigational urinary markers that have shown promise for future clinical use.

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“…55 In one study telomerase activity was found in 77% patients with transitional cell carcinoma of bladder (vs. 6% without). 56 However, in that study, there were no correlation between telomerase activity and the grade or stage of the tumor. The investigators used the TRAP assay to measure telomerase activity in urine cell pellets and noted that presence of gross hematuria was associated with false-negative results, which was resolved by washing and diluting the pellets before the assay.…”

Section: Findings Implicationsmentioning

confidence: 95%

Telomerase, Telomerase Inhibition, and Cancer

Ahmed

Tollefsbol

2003

Journal of Anti-Aging Medicine

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Telomeres, located at the ends of eukaryotic chromosomes, are synthesized by the enzyme telomerase and are responsible for maintaining chromosome length. The absence of telomerase in most somatic cells has been associated with telomere shortening and aging of these cells. In contrast, high levels of telomerase activity are observed in over 90% of human cancer cells. The absence of telomerase in normal and aging cells is considered a natural defense against development of cancer. However, we do not know what triggers the reappearance of telomerase in cancer cells. Telomerase activity is directly correlated with the expression of its active catalytic component, the human telomerase reverse transcriptase (hTERT), which is believed to be controlled primarily at the level of transcription. Elucidation of the control of telomerase in aging and in cancer as an age-related disease has considerable potential in leading to novel approaches in anti-aging medicine.

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Telomerase Activity in Diagnosis of Bladder Cancer*

Cited by 11 publications

References 16 publications

The Diagnostic Potential of Dielectric Properties, Telomerase Activity and Cytokeratin 20 in Urine Cells of Bladder Cancer Patients

The Diagnostic Potential of Dielectric Properties, Telomerase Activity and Cytokeratin 20 in Urine Cells of Bladder Cancer Patients

Current and Emerging Bladder Cancer Urinary Biomarkers

Telomerase, Telomerase Inhibition, and Cancer

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