Telomerase activation induces elongation of the telomeric single-stranded overhang, but does not prevent chromosome aberrations in human vascular endothelial cells

Anno, Kumiko; Hayashi, Akitatsu; Takahashi, Tomoko; Mitsui, Youji; Ide, Toshinori; Tahara, Hidetoshi

doi:10.1016/j.bbrc.2006.12.112

Cited by 33 publications

(33 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, total telomeres were also significantly shorter in hemodialysis patients than in control participants (Table 1). We previously found that there was a positive correlation between the lengths of telomeric G-tails and total telomeres in vitro (5). In this study, we examined the correlation between telomeric G-tail length and total telomere length in control participants and hemodialysis patients.…”

Section: Resultsmentioning

confidence: 92%

“…In fact, previous reports have shown that telomeric G-tail erosion, rather than total telomere length, serves as a trigger of cellular senescence and eventually leads to loss of cellular viability in vitro (4). We reported that telomeric G-tails in human umbilical vein endothelial cells gradually shortened with cell division and that introduction of the human telomerase reverse transcription (TERT) gene rejuvenated cellular functions (5).…”

Section: Introductionmentioning

confidence: 90%

See 1 more Smart Citation

Telomeric G-Tail Length and Hospitalization for Cardiovascular Events in Hemodialysis Patients

Hirashio

Nakashima

Doi

et al. 2014

Clinical Journal of the American Society of Nephrology

Self Cite

View full text Add to dashboard Cite

Background and objectives Telomeric G-tails play a pivotal role in maintaining the intramolecular loop structure of telomeres. Previous in vitro studies have suggested that the erosion of telomeric G-tails triggers cellular senescence, leading to organ dysfunction and atherosclerosis. The authors recently established a method to measure telomeric G-tail length using a hybridization protection assay. Using this method, this study investigated whether telomeric G-tail length could be used as a novel predictor for future cardiovascular events in hemodialysis patients.Design, setting, participants, & measurements A prospective observational study was performed involving a cohort of 203 Japanese hemodialysis patients to examine the lengths of telomeric G-tails and total telomeres and subsequent cardiovascular events during a median follow-up period of 48 months. The lengths of telomeric G-tails and total telomeres were also measured in 203 participants who did not have CKD and who were age-and sex-matched to hemodialysis patients. ResultsThe lengths of telomeric G-tails and total telomeres were significantly shorter in hemodialysis patients than in control subjects. Telomeric G-tails, but not total telomeres, were independently and negatively associated with clinical history of cardiovascular disease. During follow-up, 80 cardiovascular events occurred. Total telomere length did not predict cardiovascular events. However, the length of telomeric G-tails was associated with new-onset cardiovascular events (hazard ratio per log luminescence signals, 0.12; 95% confidence interval, 0.12 to 0.50) that persisted after adjustment for age, sex, diabetes mellitus, clinical history of cardiovascular disease, inflammation, use of vitamin D, and serum levels of phosphate and intact parathyroid hormone.Conclusions Longer telomeric G-tail length is associated with a lower risk of future cardiovascular events in hemodialysis patients.

show abstract

Section: Resultsmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 90%

Telomeric G-Tail Length and Hospitalization for Cardiovascular Events in Hemodialysis Patients

Hirashio

Nakashima

Doi

et al. 2014

Clinical Journal of the American Society of Nephrology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, it was reported that in human vascular endothelial cells, overexpression of hTERT, a catalytic component of telomerase, induces elongation of the 3 0 overhang. 21 Pot1 is a 3 0 overhang binding protein thought to play an important role in the regulation of the 3 0 overhang. 4 Elongation of the 3 0 overhang was observed in murine POT1a-deficient cells 22 and in chicken cells in which POT1 was knocked down.…”

Section: Telomericmentioning

confidence: 99%

Telomeric 3′ overhangs in chronic HBV‐related hepatitis and hepatocellular carcinoma

Lee

Choi

et al. 2008

Intl Journal of Cancer

View full text Add to dashboard Cite

Telomeric 30 overhang is a key component of telomere structure, but little is known about its role in hepatocarcinogenesis. We examined the 3 0 overhang and telomere length, mRNA levels of hTERT, POT1, TRF1 and cytokeratin 19 (CK19) in 41 hepatitis B virus (HBV)-related hepatocellular carcinomas (HCCs) and adjacent non-HCCs of B viral chronic hepatitis/cirrhosis. 3 0 overhang length was positively correlated with telomere length (p < 0.001). In non-HCCs, the 3 0 overhang shortened with increasing age (p 5 0.043). Twenty-six HCCs had shorter and 15 HCCs had longer 3 0 overhangs than the adjacent non-HCCs. The mRNA levels of hTERT, POT1 and TRF1 were upregulated in HCCs than in nonHCCs. HCCs with lengthened 3 0 overhangs expressed higher hTERT mRNA levels than those with shortened 3 0 overhangs, when compared to 3 0 overhangs in non-HCCs (p 5 0.044). POT1 and TRF1 showed no significant difference according to the 3 0 overhangs. HCCs with long 3 0 overhangs had higher mitosis (p 5 0.046) and more frequent multipolar mitosis compared to those with short 3 0 overhangs (p 5 0.034). HCCs with high cytokeratin 19 mRNA levels, a marker for hepatic progenitor cells, had longer 3 0 overhangs than HCCs with low cytokeratin 19 mRNA levels (p 5 0.019). In conclusion, the 3 0 overhang erosion might be closely related to the number of cell divisions in telomerase-negative hepatocytes of chronic hepatitis/cirrhosis. In telomerase-positive HCCs, an altered 3 0 overhang are involved in HBV-related hepatocarcinogenesis and hTERT might be involved in regulation of 3 0 overhang. ' 2008 Wiley-Liss, Inc.

show abstract

“…Immortalized human umbilical vein endothelial cells (HUVECs; HuhT1 cells) were used in this study. HuhT1 cells were previously established by transfection with human telomerase reverse transcriptase (20). HuhT1 cells was maintained in HuMedia-EG2 (Kurabo, Okayama, Japan) under the condition of 5% CO 2 in air at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…By using conditioned medium from MMP-13-overexpressing cells, we examined the migration of immortalized HUVECs. The HuhT1 cell line was previously established from HUVECs by transfection with human telomerase reverse transcriptase (20). Conditioned medium from MMP-13-overexpressing cells promoted migration of HuhT1 cells (Fig.…”

Section: Mmp-13mentioning

confidence: 99%

Matrix Metalloproteinase-13 (MMP-13) Directly and Indirectly Promotes Tumor Angiogenesis

Kudo

Iizuka

Yoshida

et al. 2012

Journal of Biological Chemistry

Self Cite

120

View full text Add to dashboard Cite

Background:Angiogenesis is an important step in the metastatic cascade of tumors. Results: MMP-13 itself as well as VEGF-A secretion from fibroblasts promotes angiogenesis. Indeed, MMP-13 is well correlated with blood vessel density in human cancer tissues. Conclusion: MMP-13 can be a marker for prediction of malignant behaviors and a therapeutic target in cancer. Significance: This work provides new insights regarding the role of MMP-13 in tumor angiogenesis.

show abstract

Telomerase activation induces elongation of the telomeric single-stranded overhang, but does not prevent chromosome aberrations in human vascular endothelial cells

Cited by 33 publications

References 20 publications

Telomeric G-Tail Length and Hospitalization for Cardiovascular Events in Hemodialysis Patients

Telomeric G-Tail Length and Hospitalization for Cardiovascular Events in Hemodialysis Patients

Telomeric 3′ overhangs in chronic HBV‐related hepatitis and hepatocellular carcinoma

Matrix Metalloproteinase-13 (MMP-13) Directly and Indirectly Promotes Tumor Angiogenesis

Contact Info

Product

Resources

About