Telocytes transfer extracellular vesicles loaded with micro<scp>RNA</scp>s to stem cells

Cismasiu, Valeriu B.; Popescu, Laurențiu M.

doi:10.1111/jcmm.12529

Cited by 93 publications

(79 citation statements)

References 47 publications

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“…Cardiac TCs have been reported to be able to regulate cardiac stem/progenitor cells, angiogenesis, and anti-fibrosis by heterocellular junctions and secreting extracellular vesicles containing microR-NAs [24,25,[44][45][46][47][48][49][50][51]. A deeper understanding of the immunocytochemical phenotypes of cardiac TCs might help better develop cardiac TCs as a novel source for cardiac repair as preliminarily demonstrated in cardiac infarction [52][53][54][55].…”

Section: Discussionmentioning

confidence: 99%

Cardiac telocytes are double positive for CD34/PDGFR‐α

Zhou

Wei

Zhong

et al. 2015

J Cellular Molecular Medi

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Telocytes (TCs) are a distinct type of interstitial cells, which are featured with a small cellular body and long and thin elongations called telopodes (Tps). TCs have been widely identified in lots of tissues and organs including heart. Double staining for CD34/PDGFR-β (Platelet-derived growth factor receptor β) or CD34/Vimentin is considered to be critical for TC phenotyping. It has recently been proposed that CD34/PDGFR-α (Platelet-derived growth factor receptor α) is actually a specific marker for TCs including cardiac TCs although the direct evidence is still lacking. Here, we showed that cardiac TCs were double positive for CD34/PDGFR-α in primary culture. CD34/PDGFR-α positive cells (putative cardiac TCs) also existed in mice ventricle and human cardiac valves including mitral valve, tricuspid valve and aortic valve. Over 87% of cells in a TC-enriched culture of rat cardiac interstitial cells were positive for PDGFR-α, while CD34/PDGFR-α double positive cells accounted for 30.25% of the whole cell population. We show that cardiac TCs are double positive for CD34/PDGFR-α. Better understanding of the immunocytochemical phenotypes of cardiac TCs might help using cardiac TCs as a novel source in cardiac repair.

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Section: Discussionmentioning

confidence: 99%

Cardiac telocytes are double positive for CD34/PDGFR‐α

Zhou

Wei

Zhong

et al. 2015

J Cellular Molecular Medi

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“…). A case in point is that when TCs are transfected with microRNA‐21 mimics labelled with Cy5, the recipient cells will become Cy5 positive after incubation with the conditioned medium from TCs, indicating that vesicles are released with microRNAs from TCs . Moreover, intestinal TCs display a phagocytic‐like property termed endocytosis, which enables themselves to uptake small particles .…”

Section: Cellular Junctions Of Tcs and Their Functional Implicationsmentioning

confidence: 99%

Telocytes in regenerative medicine

Bei

Wang

Yang

et al. 2015

J Cellular Molecular Medi

120

103

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Telocytes (TCs) are a distinct type of interstitial cells characterized by a small cell body and extremely long and thin telopodes (Tps). The presence of TCs has been documented in many tissues and organs (go to http://www.telocytes.com). Functionally, TCs form a three-dimensional (3D) interstitial network by homocellular and heterocellular communication and are involved in the maintenance of tissue homeostasis. As important interstitial cells to guide or nurse putative stem and progenitor cells in stem cell niches in a spectrum of tissues and organs, TCs contribute to tissue repair and regeneration. This review focuses on the latest progresses regarding TCs in the repair and regeneration of different tissues and organs, including heart, lung, skeletal muscle, skin, meninges and choroid plexus, eye, liver, uterus and urinary system. By targeting TCs alone or in tandem with stem cells, we might promote regeneration and prevent the evolution to irreversible tissue damage. Exploring pharmacological or non-pharmacological methods to enhance the growth of TCs would be a novel therapeutic strategy besides exogenous transplantation for many diseased disorders.

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“…Recent evidence has been found that TCs can transfer extracellular vesicles loaded with microRNAs to stem cells from the heart and blood, and this epigenetic pathway is bidirectional (Cismasiu and Popescu, 2015). In this study, some extracellular vesicles of TCs were observed by living cell imaging and TEM ( Figure S4 in Supporting Information).…”

Section: The Underlying Mechanisms By Which Tcs Promote the Proliferamentioning

confidence: 65%

“…In this study, some extracellular vesicles of TCs were observed by living cell imaging and TEM ( Figure S4 in Supporting Information). Based on previous studies, these vesicles might function as intercellular shuttles for biological signals (Cismasiu and Popescu, 2015;Manole et al, 2011). However, whether the vesicles of vascular TCs are loaded with miR-24 or other molecules requires more study.…”

Section: The Underlying Mechanisms By Which Tcs Promote the Proliferamentioning

confidence: 99%

Increased telocytes involved in the proliferation of vascular smooth muscle cells in rat carotid artery balloon injury

Zhang

Juan

et al. 2016

Sci. China Life Sci.

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Telocytes (TCs) are a novel type of interstitial cells that are thought to be involved in tissue regeneration and repair. However, the possible roles of TCs in vascular diseases remain unclear. In this study, we used a rat model of carotid artery balloon injury (CABI) to study the changes and potential roles of vascular TCs after vascular injury. Transmission electron microscopy (TEM) and CD34/vimentin immunolabeling were used to identify and quantify TCs in normal and injured carotid arteries. Quantitative immunofluorescence analysis revealed that, compared with the sham group, the number of TCs in the CABI group increased from 7.

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Telocytes transfer extracellular vesicles loaded with microRNAs to stem cells

Cited by 93 publications

References 47 publications

Cardiac telocytes are double positive for CD34/PDGFR‐α

Cardiac telocytes are double positive for CD34/PDGFR‐α

Telocytes in regenerative medicine

Increased telocytes involved in the proliferation of vascular smooth muscle cells in rat carotid artery balloon injury

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