Telocytes promote VEGF expression and alleviate ventilator-induced lung injury in mice

Ma, Ru; Wu, Pinwen; Shi, Qiqing; Song, Dongli; Fang, Hao

doi:10.1093/abbs/gmy066

Cited by 22 publications

(28 citation statements)

References 35 publications

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“…VEGF increase the vascular permeability by the endothelial cells through increasing the fenestrations 78 . Both VEGF and PDGF Receptor-α are expressed by TCs 79,80 . In the current study, VEGF was also expressed by camel efferent ductule TCs.…”

Section: Discussionmentioning

confidence: 99%

Morphological changes of telocytes in camel efferent ductules in response to seasonal variations during the reproductive cycle

Abdel-Maksoud

Abd‐Elhafeez

Soliman

2019

Sci Rep

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Telocytes (TCs) are a distinct stromal cell type described in many organs. The present study investigated the existence of TCs within the efferent ductules in camel and the changes that occur in their morphology and activity during active and inactive reproductive seasons. TCs in the camel had a cell body and multiple telopodes (TPs), and most TCs had indented nuclei that exhibited prominent intranucleolar chromatin. TCs exhibited seasonal differences which were evaluated by histochemistry, immunohistochemistry (IHC), Transimition electron microscopy (TEM) and scanning electron microscopy (SEM). The presence of TCs in camel efferent ductules has been confirmed by CD34 positive immunostaing. In addition to the expression of the vascular endothelial growth factor (VEGF) which was stronger in the summer season. TCs exhibited stronger immunoreactivity for progesterone and oestrogen alpha receptors (ESR1) in the spring than in the summer. In addition, TCs showed strong positive immunostaining for both vimentin and androgen receptor (AR). Several ultrastructural changes were observed in TCs during the two seasons. TPs in the summer season had delicate ramifications whereas, in the spring, TPs displayed fine arborization and became more corrugated. TCs acquired signs of exaggerated secretory activities in the spring; TPs became expanded and packed with secretory vesicles. Thus, we conclude that, hormonal alterations during the reproductive cycle impact the morphology and secretory behavior of TCs.

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Section: Discussionmentioning

confidence: 99%

Morphological changes of telocytes in camel efferent ductules in response to seasonal variations during the reproductive cycle

Abdel-Maksoud

Abd‐Elhafeez

Soliman

2019

Sci Rep

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“…Induced apoptosis in dermal keratinocytes can delay wound healing processes [22]. In our research, TCs non-contact co-culture reduced the LPS induced early, late and whole stages of apoptosis and cell death in HaCaT Recently study showed that cytokines, including VEGF and EGF, released by TCs are considered to be key factors were considered to be the mechanism for TCs promotion on angiogenesis [16,17].…”

Section: Discussionmentioning

confidence: 54%

“…As some groups described, TCs could secret many cytokines, including EGF and VEGF [16,17]. Since the proliferation of cells cocultured with TCs was altered, EGF was selected as the representative factor and being detected.…”

Section: Egf Mrna Level Was Increase In Tcs After Lps Stimulationmentioning

confidence: 99%

Telocytes inhibited inflammatory factor expression and enhanced cell migration in LPS-induced skin wound healing models in vitro and in vivo

et al. 2020

Self Cite

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Background: Cell proliferation and death are key components of wound healing and tissue repair. Telocytes (TCs) represent a newly discovered cell type that can protect tissue from acute injury via cell-cell communication with adjacent cells. The aim of this study was to use a mouse model of skin wound healing and lipopolysaccharide (LPS)induced cell injury to evaluate the effects of TCs on skin wound healing in vivo and in vitro. Material/methods: Immunohistochemical staining was performed to evaluate the alteration of TCs in tissues from normal and chronic wound patients. Then, a male C57BL/6 mouse wound model of the back was established. The mice were divided randomly into three groups, and wound healing was estimated according to the wound healing rate and histology. An LPS-induced co-culture model of a mouse lung telocyte cell line (TCs) with human keratinocyte (HaCaT), human dermal microvascular endothelial cell (HDMEC) or murine fibroblast (L929) cell lines was established to analyse the effects of TCs on constitutive cell types of the skin. Cell proliferation, migration and apoptosis were examined, and reactive oxygen species (ROS) and inflammatory factors in HaCaT cells, HDMECs, and L929 cells were detected to study the mechanisms involved in TC protection in skin wounds. Results: TCs were significantly increased in tissues from chronic wound patients compared with healthy controls. Wound healing was significantly improved in wound mouse models treated with exogenous TCs compared with LPSinduced models. TCs reversed the LPS-induced inhibition of HaCaT cells and HDMECs and reduced the LPS-induced apoptosis of HaCaT cells and the death ratios of HDMECs and L929 cells. TCs reversed LPS-induced ROS in HDMECs and L929 cells and decreased inflammatory factor mRNA levels in HaCaT cells, HDMECs and L929 cells. Conclusions: TCs reduce wound healing delay, and inflammatory responses caused by LPS might be mediated by inflammatory inhibition, thus restricting apoptosis and promoting migration of the main component cell types in the skin.

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“…Also, TCs secretome factors could participate in decidua formation [17]. Some studies pointed to the angiogenic properties of TCs Telocytes: New Connecting Devices in the Stromal Space of Organs DOI: http://dx.doi.org /10.5772/intechopen.89383 due to vascular endothelial growth factor (VEGF) expression [15,82,83], while others indicated the anti-oxidative properties of TCs because the TCs specific morphology can be changed by modifying the redox balance of their environment or by aging due to their richness in SOD2 (mitochondrial superoxide dismutase) [21,84].…”

Section: Telocytes In Uterus and Fallopian Tubes In Healthy Conditionmentioning

confidence: 99%

Telocytes: New Connecting Devices in the Stromal Space of Organs

Crețoiu¹,

Vannucchi²,

Bei³

et al. 2020

Innovations in Cell Research and Therapy

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Telocytes (TCs) represent a new type of interstitial cells, and were discovered by Prof. Popescu and his collaborators from Bucharest in 2005, and described as Interstitial Cajal-Like Cells (ICLCs). In 2010, Prof. Popescu and Prof. Faussone-Pellegrini from Florence, based on their expertise in morphology, agreed that in fact ICLCs were a brand-new entity and they renamed them telocytes. TCs are characterized by specific veil-or ribbon-like extensions called telopodes. Telopodes aid TCs in forming homo-or hetero-cellular contacts; thus, assembling three-dimensional networks that organizes the stromal and the parenchymal components of the organs. TCs can transfer information to neighbor cells ensuring a short-distance communication, and remotely by the release a wide variety of extracellular vesicles: exosomes, ectosomes, and multivesicular bodies. Here, we reviewed the evolution of the interest regarding TCs in different organs, in normal and pathological conditions. The main focus was on the role of TCs in gastrointestinal tract, urinary bladder, reproductive tract, and heart. This chapter sums up information about the possibilities that TCs are capable to behave as sensors/mediators in nervous activity, to represent mesenchymal stem cell precursors in adulthood, and to control and determine the differentiation/maturation of other cell types either during development or in postnatal life.

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Telocytes promote VEGF expression and alleviate ventilator-induced lung injury in mice

Cited by 22 publications

References 35 publications

Morphological changes of telocytes in camel efferent ductules in response to seasonal variations during the reproductive cycle

Morphological changes of telocytes in camel efferent ductules in response to seasonal variations during the reproductive cycle

Telocytes inhibited inflammatory factor expression and enhanced cell migration in LPS-induced skin wound healing models in vitro and in vivo

Telocytes: New Connecting Devices in the Stromal Space of Organs

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