Telocytes and Other Interstitial Cells 2.0: From Structure to Function

Crețoiu, Dragoș

doi:10.3390/ijms232416221

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…Immunoelectron microscopy is considered a gold standard for the identification of TCs. 8 Genomics and proteomics analyses further discriminated lung TCs from colonized and migrated cell types in lung tissue and were proved to be an independent cell type. [9][10][11] Immunohistochemistry studies proposed double positive immunostaining with CD34/CD117 or CD34/vimentin for identification.…”

Section: Introductionmentioning

confidence: 99%

“…19 Although several TCs subsets were identified and TCsmediated cell interaction had been demonstrated to have benefits on epithelium regeneration, we have little understanding of the molecule mechanisms in these processions. Single-cell RNA sequencing (scRNA-seq) provides us with a way to simultaneously characterize the transcriptional status of thousands of cells, 20,21 In this study, we integrated human lung scRNA-seq databases derived from five articles [6][7][8]12,22 to uncover TCs subpopulations by analyzing cells from human lung tissue in order to understand the functions of TCs profoundly. Enhanced specific signals including integrin beta 1 (ITGB1), CD44, and CD63 emphasized exosomes in the communication of TCs with other cell types.…”

Section: Introductionmentioning

confidence: 99%

“…Immunoelectron microscopy is considered a gold standard for the identification of TCs 8 . Genomics and proteomics analyses further discriminated lung TCs from colonized and migrated cell types in lung tissue and were proved to be an independent cell type 9–11 .…”

Section: Introductionmentioning

confidence: 99%

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Single‐cell RNA sequencing reveals telocytes subsets of human lung

Dai,

Cai,

Wang

et al. 2024

Clinical and Translational Dis

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BackgroundTelocyte (TC) is a recently defined renewed cell and its dominant intercommunication with other cells displays multiple functions in tissue homeostasis and diseases. Alveolar epithelial cells and immune cells were in the lung cancer heterogeneity, progression, and metastasis, and further associated with antitumor therapeutic strategies. However, few studies focus on the roles of TCs in lung cancer.MethodsIn this article, we used the public scRNA‐Seq data (including healthy control, chronic obstructive pulmonary disease, non–small cell lung cancer, lymph node metastases from non–small cell lung cancer, and systemic sclerosis–associated interstitial lung disease patients) to analyze the cellular dynamics in human lung and distinct types of TCs and their communication networks with the variety of cell types.ResultsSix subclusters of TCs were identified by expression of specific biological function markers, which demonstrated the diversity of TCs subsets in lung tissue. Further results showed TCs had communication with epithelial cells or immune cells subsets by the ligand–receptor interaction, including TIMP metallopeptidase inhibitor 1CD63, fibulin 1integrin subunit beta 1, vimentinCD44, macrophage migration inhibitory factorCD74, and amyloid beta precursor proteinCD74. Ligand–receptor interaction heterogeneity was revealed in lung tissue of healthy or diseases. Enhanced specific signals in ligandreceptor interaction were revealed, including integrin beta 1 and CD44 were appraised in the communication of TCs with epithelial cells, NK cells, NKT cells, CD4+ exhausted T cells, CD4+ memory/effector T cells, CD4+ naïve T cells, CD8+ exhausted T cells, CD8+ memory/effector T cells, and CD8+ naïve T cells. CD63, a marker identified in TCs exosomes was emphasized in our current analysis which is closely related to communication of TCs with other cell types.ConclusionThese results will provide us with new insight into the mechanisms of TCs‐dominated communication and promise therapy of TC exosomes in lung diseases.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Single‐cell RNA sequencing reveals telocytes subsets of human lung

Dai,

Cai,

Wang

et al. 2024

Clinical and Translational Dis

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“…Telocytes (TCs), which are novel and specific Interstitial Cajal‐like cells, have gained increasing attention due to their diverse functions in wound healing, cardiovascular injury, tumor growth, and uterine physiology (Cretoiu, 2021; Díaz‐Flores et al, 2021; Kondo & Kaestner, 2019). They can be identified by unique biomarker immunostaining and transmission electron microscopy scanning.…”

Section: Introductionmentioning

confidence: 99%

Matrix metalloproteinase gene mutations and bioinformatics of telocytes in hepatocellular carcinoma

Luan¹,

2022

Cell Biology International

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Telocytes (TCs) have crucial functions to promote the metastasis of hepatocellular carcinoma (HCC) by over-expressing matrix metalloproteinases (MMPs), but the mechanism by which TCs secrete MMPs in the genome is still unknown. We first cultured and isolated primary TCs from distinct liver cancer tissues and hepatic hemangioma surrounding tissues (Control group). Their whole exon genes were tested by Illumina HiSeq family of platforms and by high-throughput sequencing as well as variant mutations. Moreover, immunohistochemistry, Western blot, and quantitative reverse transcription-polymerase chain reaction assays were utilized to assess the expression of MMPs. The perniciousness of signal-nucleotide polymorphism (SNP) mutations of proteins were predicted by the Polyphen-2 database. Divergent expression and overall survival (OS) of MMPs was screened by StarBase-Pan Cancer plate; and MMPs associated signaling pathways were found by Kyoto Encyclopedia Genes and Genomes. The "competing endogenous RNA (ceRNA)" network was constructed by Cytoscape software. We found that 12 specific types of SNP mutations related to 5

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Exosomes enriched by miR-429-3p derived from ITGB1 modified Telocytes alleviates hypoxia-induced pulmonary arterial hypertension through regulating Rac1 expression

Qi,

Zhang,

Yan

2024

Cell Biol Toxicol

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Background Recent studies have emphasized the critical role of Telocytes (TCs)-derived exosomes in organ tissue injury and repair. Our previous research showed a significant increase in ITGB1 within TCs. Pulmonary Arterial Hypertension (PAH) is marked by a loss of microvessel regeneration and progressive vascular remodeling. This study aims to investigate whether exosomes derived from ITGB1-modified TCs (ITGB1-Exo) could mitigate PAH. Methods We analyzed differentially expressed microRNAs (DEmiRs) in TCs using Affymetrix Genechip miRNA 4.0 arrays. Exosomes isolated from TC culture supernatants were verified through transmission electron microscopy and Nanoparticle Tracking Analysis. The impact of miR-429-3p-enriched exosomes (Exo-ITGB1) on hypoxia-induced pulmonary arterial smooth muscle cells (PASMCs) was evaluated using CCK-8, transwell assay, and inflammatory factor analysis. A four-week hypoxia-induced mouse model of PAH was constructed, and H&E staining, along with Immunofluorescence staining, were employed to assess PAH progression. Results Forty-five miRNAs exhibited significant differential expression in TCs following ITGB1 knockdown. Mus-miR-429-3p, significantly upregulated in ITGB1-overexpressing TCs and in ITGB1-modified TC-derived exosomes, was selected for further investigation. Exo-ITGB1 notably inhibited the migration, proliferation, and inflammation of PASMCs by targeting Rac1. Overexpressing Rac1 partly counteracted Exo-ITGB1’s effects. In vivo administration of Exo-ITGB1 effectively reduced pulmonary vascular remodeling and inflammation. Conclusions Our findings reveal that ITGB1-modified TC-derived exosomes exert anti-inflammatory effects and reverse vascular remodeling through the miR-429-3p/Rac1 axis. This provides potential therapeutic strategies for PAH treatment. Graphical Abstract 1. Identification of Differentially Expressed microRNAs (DEmiRs) in ITGB1 overexpressed TCs. 2. Effects of Exo-ITGB1 or miR-429-3p on Hypoxia-Induced PASMCs in vitro. 3. Exo-ITGB1 inhibits the hyper-proliferation and migration of PASMCs through regulating miR-429-3p/Rac1 axis in vitro. 4. The therapeutic potential of Exo-ITGB1 in hypoxia-induced PAH model in vivo.

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Telocytes and Other Interstitial Cells 2.0: From Structure to Function

Abstract: Interstitial cells are often seen as those cells that fill the space between parenchymal cells, responsible for fulfilling the function of an organ [...]

Cited by 5 publications

References 23 publications

Single‐cell RNA sequencing reveals telocytes subsets of human lung

Single‐cell RNA sequencing reveals telocytes subsets of human lung

Matrix metalloproteinase gene mutations and bioinformatics of telocytes in hepatocellular carcinoma

Exosomes enriched by miR-429-3p derived from ITGB1 modified Telocytes alleviates hypoxia-induced pulmonary arterial hypertension through regulating Rac1 expression

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