Teicoplanin-Based Enantiomeric Separations in CZE Using a Partial Filling Technique

Carotti, Angelo; Gioia, Francesco Di; Cellamare, Saverio; Fanali, Salvatore

doi:10.1002/(sici)1521-4168(19990601)22:6<315::aid-jhrc315>3.0.co;2-n

Cited by 14 publications

(1 citation statement)

References 40 publications

(34 reference statements)

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“…The applicability of this approach is demonstrated with the chiral separation of 9-fluorenylmethoxycarbonyl (FMOC)-amino acids and ketoprofen. Proof of the hypothesis that the enantiorecognition of macrocyclic antibiotics takes place at the D-Ala-D-Ala binding site, located in the aglycon core, was given by Carotti et al [127]. Teicoplanin was applied in a mixture with D-Ala-D-Ala or L-Ala-L-Ala in the electrolyte using p-methoxymandelic acid as a model analyte.…”

Section: Macrocyclic Antibioticsmentioning

confidence: 99%

Recent progress in chiral separation principles in capillary electrophoresis

2000

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This review summarizes recent developments in the field of chiral separations by electromigration techniques including capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), isotachophoresis (ITP), electrokinetic chromatography (EKC), and capillary electrochromatography (CEC). This overview focuses on the development of new chiral selectors and the introduction of new techniques rather than applications of already established selectors and methods. The mechanisms of the different chiral separation principles are discussed.

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Section: Macrocyclic Antibioticsmentioning

confidence: 99%

Recent progress in chiral separation principles in capillary electrophoresis

2000

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Fundamental aspects of chiral separations by capillary electrophoresis

2001

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A review is presented that surveys the basic theory of direct separation of enantiomers by capillary electrophoretic (CE) techniques. These separations are based on the formation of diastereomeric complexes between the enantiomeric analytes and a chiral selector added to the electrolyte solution. The review covers a comprehensive treatment of the equations needed for optimization of selectivity coefficients, resolution and analysis time in the zone electrophoretic mode. In this context, it takes into account combined equilibria of complexation and protonation/deprotonation as well as complexation and paritition into micelles. On the basis of these equations, the benefits of charged selectors and the optimization potential inherent to pH tuning can be documented. In addition, the review deals with some basic aspects of chiral isoelectric focusing and briefly discusses indirect enantioseparation. In a subsequent section a survey is given on particularfeatures of the various types of chiral selectors. Finally, the recent developments in preparative enantioseparation in continuous free-flow system and by use of isoelectric membranes are discussed.

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Chiral resolution of diastereomeric di‐ and tripeptides on a teicoplanin aglycone phase by capillary electrochromatography

et al. 2003

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The chiral separation ability of a capillary packed with teicoplanin aglycone as a chiral stationary phase was investigated. This stationary phase was used successfully for chiral resolution of both diastereomeric dipeptides and tripeptides possessing one or two chiral centers. The composition of the mobile phase was shown to be crucial for separation. The use of reversed-phase mode was clearly superior to the polar-organic mode. The nature of the organic modifier was found to have a marked influence on separation. After optimizing conditions, all diasteromeric dipeptides and tripeptides investigated were baseline-resolved, however, it was not possible to find a uniform mobile phase showing optimal results for all peptides investigated.

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Teicoplanin-Based Enantiomeric Separations in CZE Using a Partial Filling Technique

Cited by 14 publications

References 40 publications

Recent progress in chiral separation principles in capillary electrophoresis

Recent progress in chiral separation principles in capillary electrophoresis

Fundamental aspects of chiral separations by capillary electrophoresis

Chiral resolution of diastereomeric di‐ and tripeptides on a teicoplanin aglycone phase by capillary electrochromatography

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