Tectorigenin, a Flavonoid-Based Compound of Leopard Lily Rhizome, Attenuates UV-B-Induced Apoptosis and Collagen Degradation by Inhibiting Oxidative Stress in Human Keratinocytes

Abstract

Ultraviolet (UV) light, a major risk factor for external skin photoaging, induces oxidative stress in skin. UV causes a breakdown of skin homeostasis by impairing the extracellular matrix and inducing cell death. Tectorigenin, a constituent of leopard lily (Belamcanda chinensis L.) rhizome, has been reported to possess antioxidant, hair-darkening, and anti-inflammatory activities; however, the effect of tectorigenin on UV-B-induced skin damage is unknown. Here, we investigated the anti-skin-damage effects of tectorigenin against UV-B-stimulated oxidative stress in human keratinocytes. We irradiated HaCaT cells with UV-B (25 mJ/cm2), followed by treatment with tectorigenin for 24 h. We found that tectorigenin decreased the levels of intracellular reactive oxygen species by increasing the expression of anti-oxidative enzymes, such as glutathione and catalase. Furthermore, tectorigenin inhibited apoptosis by reducing caspase-3- and Bcl-2-associated protein-X levels, and increasing Bcl-2 protein levels. Tectorigenin also decreased matrix metalloproteinase-1 levels and increased type 1 collagen levels, thus preventing collagen degradation. These data demonstrate that tectorigenin exerts anti-skin-damage effects in human keratinocytes by attenuating UV-B-induced hyper-oxidation, apoptosis, and collagen degradation.

Disease-Dependent Antiapoptotic Effects of Cannabidiol for Keratinocytes Observed upon UV Irradiation

“…The UV irradiation enhances ROS production and activates proinflammatory pathways, including biosynthesis of prostaglandins and “the death ligands”, especially TNFα, which activates the extrinsic pathway of apoptosis through caspase 8 [ 55 ]. Other authors also point to the role of caspases 3 and 8 in the apoptosis of keratinocytes from healthy people after exposure to UVB irradiation [ 56 , 57 ], but so far there have been no data on the response of psoriatic keratinocytes. The results of our study indicate that UVB leads mainly to the activation of the extrinsic apoptotic pathway in healthy and psoriatic cells, likely due to its impact on the level of proapoptotic 15-d-PGJ 2 .…”

Tectorigenin regulates migration, invasion, and apoptosis in dexamethasone‐induced human airway epithelial cells through up‐regulating miR‐222‐3p

“…Bax activates caspase‐3 and induces the release of cytochrome c in mitochondria. The activated caspase‐3 is Cleaved Caspase‐3 (Noh et al, 2018). Studies have shown that Tectorigenin inhibits apoptosis by suppressing caspase‐3 and Bax levels and promoting Bcl‐2 protein level (Noh et al, 2018).…”

“…The activated caspase‐3 is Cleaved Caspase‐3 (Noh et al, 2018). Studies have shown that Tectorigenin inhibits apoptosis by suppressing caspase‐3 and Bax levels and promoting Bcl‐2 protein level (Noh et al, 2018). Our results showed that Dex promoted the expressions of Bax and Cleaved Caspase‐3, but inhibited the expression of Bcl‐2, which was further reversed by Tectroigenin.…”

“…MiRNAs plays crucial roles in pathogenesis and wound repair process of asthma, miR‐222‐3p activates MAPK signaling pathway to promote the viability, migration of airway epithelial cells and the repair of GCs‐induced airway epithelial injury (Noh et al, 2018; Xu et al, 2020). However, the relationship between Tectorigenin and miR‐222‐3p in asthma currently remained unclear.…”

“…It has been previously revealed that many natural products, such as Panax notoginseng saponins R1 and Tectorigenin, possess regulatory effects on improving asthma (Pan et al, 2008). Tectorigenin is a substance isolated from leopard lily ( Belamcanda chinensis L.) rhizome and belongs to O ‐methylated isoflavone (Noh et al, 2018). It can act as a transmitter to scavenge oxygen free radicals, thus playing an important role in protecting liver and reducing ischemia–reperfusion injury (Pan et al, 2008; Zeng et al, 2018).…”