Técnicas de laboratório

Moura, Rafael de Deus

doi:10.1590/s0036-46651991000400020

Cited by 30 publications

(44 citation statements)

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“…Serum glucose was quantified enzymatically using the glucose oxidase and peroxidase method (Moura 1982) The serum concentration of total cholesterol was determined by enzymatic methods using cholesterol ester/oxidase. The serum high-density lipoprotein (HDL) content was determined in the supernatant fraction after precipitation of the very low-density lipoproteins (VLDL) and low-density lipoproteins (LDL) with phosphotungstic acid and MgCl 2 .…”

Section: Measurement Of Glycaemia and Dyslipidaemiamentioning

confidence: 99%

Evaluation of lipid profile and oxidative stress in STZ-induced rats treated with antioxidant vitamin

Almeida

Braga

Novelli

et al. 2012

Braz. arch. biol. technol.

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The present study investigated the effect of supplementation of vitamin E on streptozotocin (STZ)-induced diabetic rats by measuring blood glucose, changes in body weight, food and water intake, lipid profile, serum urea and creatinine level, and antioxidant enzyme activity. Male Wistar rats were divided into four groups: control rats (GI); rats receiving vitamin E (GII); STZ-induced diabetic rats (GIII) and STZ-induced diabetic rats treated with vitamin E (GIV). Vitamin E reduced (p<0.05) blood glucose and urea, improved the lipid profile (decreased the serum levels of total cholesterol, LDL cholesterol, VLDL cholesterol and triacylglycerols, and increased HDL cholesterol) and increased total protein in STZ-induced diabetic rats (GIV). Vitamin prevented changes in the activity of SOD and GSH-Px and in the concentration of lipid hydroperoxide. These results suggested that vitamin E improved hyperglycaemia and dyslipidaemia while inhibiting the progression of oxidative stress in STZ-induced diabetic rats.

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Section: Measurement Of Glycaemia and Dyslipidaemiamentioning

confidence: 99%

Evaluation of lipid profile and oxidative stress in STZ-induced rats treated with antioxidant vitamin

Almeida

Braga

Novelli

et al. 2012

Braz. arch. biol. technol.

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“…Zymosan particles preincubated together with lipoproteins or zymosan particles alone (control) were added to the cells that adhered to the coverslips and incubated for an additional 30 min at 37 o C in a humid 5% CO 2 atmosphere. The coverslips were then washed with pre-warmed RPMI 1640 medium to remove excess particles, stained with Leishman solution (15) and read under the light microscope. The ANOVA test was used for statistical comparison of the percentage of macrophages that phagocytized zymosan particles; a 0.05-confidence level was considered significant.…”

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confidence: 99%

Zymosan phagocytosis by mouse peritoneal macrophages is increased by apoHDL- and not by intact HDL-covered particles

Carvalho

Tobias

Vendrame

et al. 2000

Braz J Med Biol Res

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The uptake of lipids and lipoprotein particles by macrophages undergoes phagocytic activation and the formation of foam cells are key events in atherosclerosis. In this study we determined how intact high density lipoproteins (HDL) and apolipoproteins-HDL (removal of the lipid component from HDL, i.e., apoHDL) influence the phagocytosis of zymosan by mouse peritoneal macrophages. Zymosan particles preincubated together with lipoproteins or alone (control) were incubated with the macrophages. Phagocytosis activity was reported as the percent of macrophages that internalized three or more zymosan particles. HDL co-incubated with zymosan did not influence the overall uptake of zymosan particles compared to apoHDL, which greatly enhanced the ability of the particle to be phagocytized (P<0.001). Part of this effect might be related to a greater binding of apoHDL to the particles compared to that of HDL (P<0.05). We conclude that this can be a useful method to study the ability of lipoproteins, including modified lipoproteins obtained from subjects with genetic forms of hyperlipidemia, to opsonize particles such as red blood cells and thus to investigate the processes that control the formation of foam cells and the mechanisms of atherogenesis.

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“…O número de grãos de pólen por antera foi estimado utilizando-se uma câmara de Neubauer (Moura et al 1987).…”

Section: Methodsunclassified

Biologia floral e sistema de polinização de Solanum stramonifolium Jacq. (Solanaceae) em remanescente de Mata Atlântica, Pernambuco

Bezerra

Machado

2003

Acta Bot. Bras.

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). A deiscência de anteras através de pequenos poros apicais é uma característica encontrada em várias espécies da família Solanaceae, especialmente no gênero Solanum, característica esta que restringe a polinização a um grupo de abelhas fêmeas capazes de vibrar as anteras para retirada do pólen. A fenologia, biologia floral e a polinização de Solanum stramonifolium foram estudadas entre os meses de agosto/1999 a dezembro/2000 em populações naturais ocorrentes no Parque Estadual de Dois Irmãos, um dos poucos remanescentes de Mata Atlântica localizada em Recife, Pernambuco (8°7'30"S e 34°52'30"W). Solanum stramonifolium é um arbusto com flores dispostas em inflorescências racemosas, apresentando corola branca e cinco estames de um amarelo intenso, com anteras poricidas dispostas ao redor do gineceu. A antese ocorre nas primeiras horas do dia, havendo reflexão de luz ultravioleta e presença de áreas de concentração de emissão de odor por toda a corola e na região apical das anteras. Solanum stramonifolium tem padrão de floração contínuo e apresenta flores hermafroditas (62%) e funcionalmente masculinas (38%), o que caracteriza a espécie como andromonóica. Treze espécies de abelhas foram observadas visitando as flores de S. stramonifolium (nove polinizadores e quatro pilhadores). A constante e grande produção de flores faz com que Solamun stramonifolium mantenha sua guilda de polinizadores e garanta assim a sua reprodução, sendo, ao mesmo tempo, uma importante fonte de recursos para a manutenção destas abelhas.Palavras-chave -Solanum, polinização vibrátil, andromonoicia, Floresta Atlântica, melitofilia ABSTRACT -(Floral biology and pollination system of Solanum stramonifolium Jacq. (Solanaceae) in an Atlantic Forest remnant in Pernambuco). The anthers deiscense through two small apical pores is a feature found in many species of Solanaceae, especially in the genus Solanum. This feature restricts pollination to a group of female bees which are able to vibrate the anthers (buzz pollination). The phenology, floral biology and pollination of Solanum stramonifolium were studied between August/1999 and December/2000

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Técnicas de laboratório

Cited by 30 publications

References 0 publications

Evaluation of lipid profile and oxidative stress in STZ-induced rats treated with antioxidant vitamin

Evaluation of lipid profile and oxidative stress in STZ-induced rats treated with antioxidant vitamin

Zymosan phagocytosis by mouse peritoneal macrophages is increased by apoHDL- and not by intact HDL-covered particles

Biologia floral e sistema de polinização de Solanum stramonifolium Jacq. (Solanaceae) em remanescente de Mata Atlântica, Pernambuco

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