Técnicas de conservación in vitro para el establecimiento de bancos de germoplasma en cultivos tropicales.

Sánchez-Chiang, Neiva; Jiménez, Víctor M.

doi:10.15517/am.v21i1.11836

Cited by 12 publications

(9 citation statements)

References 7 publications

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“…In vitro preservation: in vitro storage is an essential part of the overall strategy for the preservation and exchange of plant genetic resources worldwide [ 22 ]. In vitro germplasm banks conserve plant genetic resources under controlled aseptic conditions and involve various cell and tissue culture techniques, as well as synthetic seeds [ 23 ]. In vitro culture of slow growth is widely used to obtain medium-term storage of tropical species explants, crops with vegetative propagation (e.g., potato, banana, cassava), or endangered species [ 24 ].…”

Section: Preservation Of Plant Genetic Resourcesmentioning

confidence: 99%

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Roque‐Borda

Kulus

Souza

et al. 2021

IJMS

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Numerous environmental and endogenous factors affect the level of genetic diversity in natural populations. Genetic variability is the cornerstone of evolution and adaptation of species. However, currently, more and more plant species and local varieties (landraces) are on the brink of extinction due to anthropopression and climate change. Their preservation is imperative for the sake of future breeding programs. Gene banks have been created worldwide to conserve different plant species of cultural and economic importance. Many of them apply cryopreservation, a conservation method in which ultra-low temperatures (−135 °C to −196 °C) are used for long-term storage of tissue samples, with little risk of variation occurrence. Cells can be successfully cryopreserved in liquid nitrogen (LN) when the adverse effect of ice crystal formation and growth is mitigated by the removal of water and the formation of the so-called biological glass (vitrification). This state can be achieved in several ways. The involvement of key cold-regulated genes and proteins in the acquisition of cold tolerance in plant tissues may additionally improve the survival of LN-stored explants. The present review explains the importance of cryostorage in agronomy and presents an overview of the recent works accomplished with this strategy. The most widely used cryopreservation techniques, classic and modern cryoprotective agents, and some protocols applied in crops are considered to understand which parameters provide the establishment of high quality and broadly applicable cryopreservation. Attention is also focused on the issues of genetic integrity and functional genomics in plant cryobiology.

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Section: Preservation Of Plant Genetic Resourcesmentioning

confidence: 99%

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Roque‐Borda

Kulus

Souza

et al. 2021

IJMS

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“…These pressures justify the development of a sustainable means of T. domingensis production to preserve its environmental services and benefits ( Liu et al, 2012 ; He et al, 2015 ), such as a method of propagation that enables T. domingensis extraction independent from the natural environment. For aquatic monocotyledons, with the exception of rice ( Indoliya et al, 2016 ), in vitro methods of asexual or somatic embryogenesis to conserve and propagate germplasm and to achieve the sustainable production of genetic varieties ( Von Arnold et al, 2002 ; Sánchez-Chiang & Jiménez, 2010 ; Reed et al, 2011 ) have rarely been applied. Investigations of somatic embryos (SEs) have contributed greatly to understanding the physiological, biochemical, and molecular mechanisms underlying sexual embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

Morpho-histological development of the somatic embryos of Typha domingensis

Hernández-Piedra

Ruiz-Carrera

Sánchez

et al. 2018

PeerJ

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BackgroundSustainable methods of propagation of Typha domingensis through somatic embryogenesis can help mitigate its current condition of ecological marginalization and overexploitation. This study examined whether differentiation up to coleoptilar embryos could be obtained in an embryogenic line proliferated with light and high auxin concentration.MethodsMurashige and Skoog medium at half ionic strength and containing 3% sucrose and 0.1% ascorbic acid was used for the three embryogenic phases. Induction started with aseptic 9-day-old germinated seeds cultured in 0.5 mg L−1 2,4-dichlorophenoxyacetic (2,4-D). Proliferation of the embryogenic callus was evaluated at 2,4-D concentrations ranging from 0 to 2 mg L−1 in cultures maintained in the dark. The dominant embryogenic products obtained in each treatment were used as embryogenic lines in the third phase. Thus, maturation of the somatic embryos (SEs) was analyzed using four embryogenic lines and under light vs. dark conditions. Embryogenic differentiation was also monitored histologically.ResultsProliferation of the nine morphogenetic products was greater in the presence of 2,4-D, regardless of the concentration, than in the absence of auxin. Among the products, a yellow callus was invariably associated with the presence of an oblong SE and suspended cells in the 2,4-D treatments, and a brown callus with scutellar somatic embryos (scSEs) in the treatment without 2,4-D. During the maturation phase, especially the embryogenic line but also the light condition resulted in significant differences, with the highest averages of the nine morphogenetic products obtained under light conditions and the maximum concentration of auxin (YC3 embryogenic line). Only this line achieved scSE growth, under both light and dark conditions. Structurally complete coleoptilar somatic embryos (colSEs) could be anatomically confirmed only during the maturation phase.DiscussionIn the embryogenic line cultured with the highest auxin concentration, light exposure favored the transdifferentiation from embryogenic callus to scSE or colSE, although growth was asynchronous with respect to the three embryogenic phases. The differentiation and cellular organization of the embryos were compatible with all stages of embryogenic development in other monocotyledons. The growth of colSEs under light conditions in the YC3 embryogenic line and the structurally complete anatomic description of colSEs demonstrated that differentiation up to coleoptilar embryos could be obtained. The diversity of embryogenic products obtained in the YC3 embryogenic line opens up the opportunity to synchronize histological descriptions with the molecules associated with the somatic embryogenesis of Typha spp.

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“…Currently, in vitro technology for the conservation of oil palm germplasm is focused almost exclusively on the technique of cryopreservation of zygotic embryos (Villa et al, 2007), somatic embryos (Konan et al, 2010), and pollen (Tandon et al, 2007). However, despite its relative effectiveness, cryopreservation requires a highly-specialized structure and labor, and it is a technique aimed at long-term conservation (Sánchez-Chiang & Jiménez, 2010). A complementary alternative is in vitro maintenance of whole plants, for the purpose of making the germplasm available at short and medium terms.…”

mentioning

confidence: 99%

“…A complementary alternative is in vitro maintenance of whole plants, for the purpose of making the germplasm available at short and medium terms. However, for this technique to be truly effective, it is necessary to adapt the used methodologies, by determining the best formulation of the culture media, the number of samples to be conserved, the frequency of the subcultures, and the optimal temperature and light conditions for the maintenance of in vitro collections (Sánchez-Chiang & Jiménez, 2010;Scherwinski-Pereira et al, 2010). For that reason, studies on in vitro conservation of whole oil palm plants are still incipient.…”

mentioning

confidence: 99%

In vitro maintenance, under slow-growth conditions, of oil palm germplasm obtained by embryo rescue

Camillo

Scherwinski‐Pereira

2015

Pesq. agropec. bras.

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-The objective of this work was to evaluate the in vitro maintenance of oil palm (Elaeis guineensis and E. oleifera) accessions under slow-growth conditions. Plants produced by embryo rescue were subject to ½MS culture medium supplemented with the carbohydrates sucrose, mannitol, and sorbitol at 1, 2, and 3% under 20 and 25±2ºC. After 12 months, the temperature of 20°C reduced plant growth. Sucrose is the most appropriate carbohydrate for maintaining the quality of the plants, whereas mannitol and sorbitol result in a reduced plant survival.

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Técnicas de conservación in vitro para el establecimiento de bancos de germoplasma en cultivos tropicales.

Cited by 12 publications

References 7 publications

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Morpho-histological development of the somatic embryos of Typha domingensis

In vitro maintenance, under slow-growth conditions, of oil palm germplasm obtained by embryo rescue

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