Technology Renews a Basic Approach

Arnaud, Celia

doi:10.1021/cen-v089n003.p013

Cited by 10 publications

(8 citation statements)

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“…Numerous drugs in dried blood spots, including dextrorphan, amitriptyline, imipramine, citalopram, and imatinib, have been directly analyzed from paper substrates using paper spray. This demonstrated that paper spray can be used as an effective alternative to standard extraction procedures[42] and also to newer methods including DESI [43] and liquid microjunction surface sampling probes (LMJ-SSP)[44]. Linear responses for these drugs were recorded by PS-MS across a concentration range of at least three orders of magnitude, fully covering their therapeutic ranges[39].…”

Section: Introductionmentioning

confidence: 99%

Paper spray ionization devices for direct, biomedical analysis using mass spectrometry

Yang

Wang

Maas

et al. 2012

International Journal of Mass Spectrometry

182

166

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Paper spray ionization has been developed as a direct, fast and low-cost sampling and ionization method for qualitative and quantitative mass spectrometric (MS) analysis of complex mixtures. Analyte ions are generated by applying a high voltage and a small volume (~10 μL) of spray solvent onto a porous substrate. The sample can be preloaded onto the paper or mixed into the spray solution. The geometry of the paper and the method of supplying the necessary internal standard are important factors that affect the ionization efficiency and subsequently the sensitivity and quantitation accuracy of the analytical data. As the cut angle of the paper tip is changed, the spray plume, the total spray current and the electric field intensity at the tip all vary correspondingly, with resulting differences in signal intensity. Sample load is another important factor for obtaining a stable MS signal and accurate quantitative results. The optimal sample load was found to be dependent on the paper size. The dissolution and spray process was also investigated and analyte transfer on paper was shown to be largely associated with bulk solution flow towards the spray tip. The information gathered from these systematic studies provides guidance for the design and optimization of a disposable sample cartridge for paper spray MS, a device which potentially is suitable for fast clinical analysis, especially for point-of-care diagnostics.

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Section: Introductionmentioning

confidence: 99%

Paper spray ionization devices for direct, biomedical analysis using mass spectrometry

Yang

Wang

Maas

et al. 2012

International Journal of Mass Spectrometry

182

166

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“…Furthermore the application of the method to dried samples (dried-blood spots, DBSs), a method developed by Guthrie in 1963, 9 would facilitate long range transportation and storage of such samples. 10,11 To the best of our knowledge, Dua et al and Burse et al were the rst to briey report preliminary data on the potential use of human DBSs for hexachlorocyclohexane (HCH), DDT, and DDE measurement using GC coupled to a non-selective micro-electron capture detector (mECD). 12,13 POP measurements in a small sample volume have also been investigated in 0.5 mL plasma samples, 14 in regular newborn screening program (NSP) samples, 1,[15][16][17] and also in animal samples.…”

Section: Introductionmentioning

confidence: 99%

Cryogenic zone compression GC-HRTOFMS for the measurement of PCB-153 and DDE in 20 μL serum samples

L'Homme

Focant

2016

Anal. Methods

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“…Dried blood spot (DBS) samples stored on filter paper are surging in popularity for applications ranging from screening for genetic disorders in newborn patients 1 to point-of-care testing for infectious diseases 2 to metabolic profiling in drug discovery and lead validation. 3 An example of the former application is the quantification of succinylacetone (SA) in DBS samples collected from newborn patients as a biochemical hallmark for tyrosinemia type 1, also known as hepatorenal tyrosinemia (HT). 4 In such tests, a punch from a DBS is preextracted in methanol and then the SA (which remains in the residual spot) is extracted and derivatized with hydrazine to form a hydrazone derivative that can be quantified using tandem mass spectrometry (MS/MS).…”

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confidence: 99%

“…Although there is great interest in developing new techniques for analyzing DBS samples, 9−13 there are few automated solutions available, and most methods used now are manual, tedious, and slow. 3 We report here a new method to analyze DBS samples for SA and other analytes. This new method is powered by digital microfluidics (DMF), a fluidhandling technique in which discrete droplets are manipulated on an open surface by applying a series of electric potentials to an array of electrodes.…”

mentioning

confidence: 99%

“…Dried blood spot (DBS) samples stored on filter paper are surging in popularity for applications ranging from screening for genetic disorders in newborn patients to point-of-care testing for infectious diseases to metabolic profiling in drug discovery and lead validation . An example of the former application is the quantification of succinylacetone (SA) in DBS samples collected from newborn patients as a biochemical hallmark for tyrosinemia type 1, also known as hepatorenal tyrosinemia (HT) .…”

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confidence: 99%

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Dried Blood Spot Analysis by Digital Microfluidics Coupled to Nanoelectrospray Ionization Mass Spectrometry

et al. 2012

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Dried blood spot (DBS) samples on filter paper are surging in popularity as a sampling and storage vehicle for a wide range of clinical and pharmaceutical applications. For example, a DBS sample is collected from every baby born in the province of Ontario, Canada, for quantification of approximately one hundred analytes that are used to screen for 28 conditions, including succinylacetone (SA), a marker for hepatorenal tyrosinemia. Unfortunately, the conventional methods used to evaluate DBS samples for newborn screening and other applications are tedious and slow, with limited options for automated analysis. In response to this challenge, we have developed a method to couple digital microfluidics (DMF) to nanoelectrospray ionization mass spectrometry (nESI-MS) for SA quantification in DBS samples. The new system is formed by sandwiching a pulled glass capillary emitter between the two DMF substrates such that the capillary emitter is immobilized without external seals or gaskets. Moreover, we introduce a new feedback control system that enables high-fidelity droplet manipulation across DBS samples without manual intervention. The system was validated by application to on-chip extraction, derivatization, and analysis of SA and other analytes from DBS samples, with comparable performance to gold-standard methods. We propose that the new methods described here can potentially contribute to a new generation of analytical techniques for quantifying analytes in DBS samples for a wide range of applications.

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Technology Renews a Basic Approach

Cited by 10 publications

References 0 publications

Paper spray ionization devices for direct, biomedical analysis using mass spectrometry

Paper spray ionization devices for direct, biomedical analysis using mass spectrometry

Cryogenic zone compression GC-HRTOFMS for the measurement of PCB-153 and DDE in 20 μL serum samples

Dried Blood Spot Analysis by Digital Microfluidics Coupled to Nanoelectrospray Ionization Mass Spectrometry

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