Technology for the production of Lyophilizate of an association of Lactic acid bacteria included in the Medicinal product AS-Probionorm

Abstract

The study aims to develop the technology of producing lyophilizate association of lactic acid bacteria included in the AS-Probionorm medicinal product. The association for obtaining the lyophilizate includes the following cultures of microorganisms – Lactobacillus fermentum 30 +Lactobacillus cellobiosus 36. It is found that to obtain a lyophilizate of the association of lactic acid bacteria included in AS-Probionorm, the best medium for cultivation is MRS with CoCl2. The optimal variant of lyophilic drying involves shelf temperature equalization (+20°C) – 5min, freezing (-30°C) – 10h, freezing (-60°C) – 5h, vacuum – 0.2mbar, drying 1(-26°C) – 6h, drying 2(+20°C) – 18h, drying 3(+30°C) – 2h with the entire process lasting at least 26hours and the final product temperature being +(25-27)°C. This procedure produces lyophilizates of the lactic acid bacteria association with high indicators of bacterial viability and antagonistic activity upon drying and after storage. Following the developed technology, the production of this effective probiotic drug against intestinal and associated infections will be established in Kazakhstan for domestic use, as well as for export to other countries.