Technologies, Tools, and Genetic Models to Study Osteoclasts

Vacher, Jean; Saad, Lina; Pata, Monica

doi:10.1016/b978-0-12-801238-3.11169-9

Cited by 1 publication

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“…Moreover, as these cells are terminally differentiated and non-proliferative, a large number of cells have to be isolated at once. However, despite these impediments, osteoclast specific markers have been defined and novel efficient tools have been developed to analyze osteoclast biology ex vivo and in vivo [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Ostm1 from Mouse to Human: Insights into Osteoclast Maturation

Vacher

Bruccoleri

Pata

2020

IJMS

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The maintenance of bone mass is a dynamic process that requires a strict balance between bone formation and resorption. Bone formation is controlled by osteoblasts, while osteoclasts are responsible for resorption of the bone matrix. The opposite functions of these cell types have to be tightly regulated not only during normal bone development, but also during adult life, to maintain serum calcium homeostasis and sustain bone integrity to prevent bone fractures. Disruption of the control of bone synthesis or resorption can lead to an over accumulation of bone tissue in osteopetrosis or conversely to a net depletion of the bone mass in osteoporosis. Moreover, high levels of bone resorption with focal bone formation can cause Paget’s disease. Here, we summarize the steps toward isolation and characterization of the osteopetrosis associated trans-membrane protein 1 (Ostm1) gene and protein, essential for proper osteoclast maturation, and responsible when mutated for the most severe form of osteopetrosis in mice and humans.

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Section: Introductionmentioning

confidence: 99%