Technologies and strategies for ex situ conservation of aquatic organisms: The role of cryopreservation in long-term management

Cabrita, Elsa; Horváth, Ákos; Marinović, Zoran; Asturiano, Juan F.

doi:10.1016/b978-0-12-822273-7.00011-2

Cited by 3 publications

(9 citation statements)

References 210 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The first step to achieving success in a cryopreservation protocol is choosing the correct container to store the sperm at low temperatures. The composition, thickness, and mainly the volume of sperm that the container holds directly influence the freezing and thawing curves of the sample and the purpose of using the samples (Cabrita et al, 2022).…”

Section: Recipientsmentioning

confidence: 99%

“…Cryotubes are used to cryopreserve sperm in higher volumes than those supported by straws (Cabrita et al, 2001). These containers are also used in germ stem cells and reproductive tissues cryopreservation protocols (Cabrita et al, 2022). Cryotubes are resistant, easily labeled, and store a high volume of cells.…”

Section: Recipientsmentioning

confidence: 99%

“…In fish sperm cryopreservation protocols, the choice and concentration of permeable cryoprotectants, the addition of cryoprotectants temperature, and the equilibrium time should be practiced assertively. Thus, the cryoprotectant solution will not be toxic to the cells and will promote efficient protection during cryopreservation (Cabrita et al, 2022). Two of the most commonly used permeable cryoprotectants in fish sperm cryopreservation protocols are dimethyl sulfoxide (Me2SO) and methanol (MeOH).…”

Section: Cryoprotectantsmentioning

confidence: 99%

“…Cryopreservation is a biotechnique that maintains cells and tissues at extremely low temperatures, ensuring their functionality, development, and growth can be preserved even after thawing, with minimal loss of efficiency. Among its various applications, fish sperm cryopreservation is a valuable tool for enhancing aquaculture practices, particularly in optimizing broodstock management at hatcheries while safeguarding natural fish stocks (Asturiano et al, 2017;Cabrita et al, 2022). Nowadays, several protocols are established for the cryopreservation of fish sperm (Cabrita et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Among its various applications, fish sperm cryopreservation is a valuable tool for enhancing aquaculture practices, particularly in optimizing broodstock management at hatcheries while safeguarding natural fish stocks (Asturiano et al, 2017;Cabrita et al, 2022). Nowadays, several protocols are established for the cryopreservation of fish sperm (Cabrita et al, 2022). Most protocols use plastic straws (0.25, 0.5, 1, 2 and 5 mL) or cryovials (1, 2, 3 and 5 mL) as sperm storage containers.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Development and application of low-cost and environment-friendly techniques for fish sperm cryopreservation

de Souza França

View full text Add to dashboard Cite

Fish sperm cryopreservation is a technique that can increase the reproduction in captive efficiency of freshwater and marine fishes. The technique is applied in environmental conservation programs and aquaculture. Over the last few decades, protocols for sperm cryopreservation from many fishes have been established. However, the researchers' main focus was successfully freezing and thawing sperm, neglecting the period in which the gametes remain in contact with the cryoprotective solution until fertilization. Exposure of sperm to cryoprotectant solutions after thawing can be harmful to the quality of the gametes since they can be toxic.The vast majority of the established protocols use ultra-resistant plastic containers to store sperm during the cryopreservation process. These containers usually are not reused, generating highly polluting waste for the environment. Furthermore, in some countries, the containers usually used are sold by a few industries, which makes acquisition difficult and increases the product's price. Thus, the main objective of the thesis was to create and test low-cost methodologies that enhance the use of fish post-thaw sperm and make the sperm cryopreservation process more environmentally friendly.The experiments in the Chapters 1 and 2 were developed in Brazil. We used the South American silver catfish Rhamdia quelen, a species considered an experimental model for native South American fishes. In vitro fertilization and sperm cryopreservation protocols for the species were already established; thus, we based our work on these protocols to apply innovations.In Chapter 1, we tested the use of post-thawing dilution to reduce the toxicity of the cryoprotectant solution. This technique is commonly used in mammalian sperm cryopreservation protocols but has never before been applied to post-thaw sperm of South American fishes. South American silver catfish post-thaw sperm samples were diluted in a saline extender (1.1% NaCl -325 mOsm kg -1 ; pH 7.6). The post-thaw sperm diluted samples showed higher velocities, straightness, progression, and flagellar beat frequency than SUMMARY 2 the cells of undiluted samples (control). The post-thawing dilution also provided higher fertilization and hatching rates than the control group. Thus, the post-thawing sperm dilution proved to be a simple, cheap, and efficient methodology that should be included in the silver catfish sperm cryopreservation protocol.

show abstract

Section: Recipientsmentioning

confidence: 99%

Section: Recipientsmentioning

confidence: 99%

Section: Cryoprotectantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Development and application of low-cost and environment-friendly techniques for fish sperm cryopreservation

de Souza França

View full text Add to dashboard Cite

show abstract

La pandemia de COVID-19 como oportunidad para poner de relieve la migración de los trabajadores agrícolas a través de la cobertura mediática

Șerban

Croitoru

2022

Estud. geogr.

View full text Add to dashboard Cite

En los últimos años, el interés por las representaciones mediáticas de los emigrantes y los medios de comunicación como espacio de participación ha aumentado dentro del campo de los estudios migratorios. Sin embargo, la mayor parte de la atención de los estudiosos se centra en los inmigrantes y los medios de comunicación en los países de destino, mientras que se presta menos atención a los países de origen y a la representación de los emigrantes. Aprovechando la mayor atención prestada a los emigrantes y a la migración durante la primera fase de la pandemia del COVID-19, investigamos las representaciones mediáticas de los emigrantes rumanos en la agricultura que trabajan en otros países europeos e interpretamos cómo sus voces podían ser escuchadas a través de los relatos mediáticos. Mediante un análisis de contenido, investigamos una muestra de 297 artículos publicados entre el 1 de abril y el 31 de mayo de 2020 en los sitios web de los seis medios de comunicación rumanos más visibles. Este estudio contribuye al conocimiento existente sobre las representaciones mediáticas de los migrantes rumanos al documentar una serie de tendencias, incluyendo un enfoque orientado a los acontecimientos, representaciones excesivamente simplificadas de la migración, masificación y esquematización de las representaciones de los migrantes, y la alta sensibilidad a los informes de los medios de comunicación de los países de destino sobre los migrantes rumanos. Nuestro análisis revela que el enfoque adoptado para informar sobre la migración durante la pandemia del COVID-19, al menos durante su primera fase, dependió en gran medida de los modos existentes e institucionalizados de informar sobre la migración en los medios de comunicación.

show abstract

Development and Application of Techniques for the Control of Captive Breeding in Elasmobranchs

García Salinas

View full text Add to dashboard Cite

Appearing 420 million years ago, sharks and rays constitute an ancient and ecologically diverse group of aquatic vertebrates known as elasmobranchs. This diverse group possesses life strategies that make them highly vulnerable to rapid environmental changes, such as those resulting from anthropogenic actions. Despite being key elements in regulating the ecosystems in which they live, they are currently one of the most threatened vertebrate groups on the planet.In parallel with in situ conservation, ex situ conservation programs can be used to improve the situation of some sensitive species. Among these programs, captive breeding plans would increase the sustainability of public aquariums and research centres, as well as allow the development of conservation strategies in the wild. However, to be effective, these plans should include the use of assisted reproductive control techniques. Unfortunately, these techniques have been scarcely developed in the past, so their usefulness has not been adequately proven.

show abstract

Technologies and strategies for ex situ conservation of aquatic organisms: The role of cryopreservation in long-term management

Cited by 3 publications

References 210 publications

Development and application of low-cost and environment-friendly techniques for fish sperm cryopreservation

Development and application of low-cost and environment-friendly techniques for fish sperm cryopreservation

La pandemia de COVID-19 como oportunidad para poner de relieve la migración de los trabajadores agrícolas a través de la cobertura mediática

Development and Application of Techniques for the Control of Captive Breeding in Elasmobranchs

Contact Info

Product

Resources

About