Technological-economic optimization of enzymatic hydrolysis used for the processing of chrome-tanned leather waste

Pecha, Jiří; Bařinová, Michaela; Kolomaznı́k, Karel; Nguyen, Thanh Nhu; Dao, Anh Tuan; Le, Van Thi

doi:10.1016/j.psep.2021.06.009

Cited by 14 publications

(4 citation statements)

References 31 publications

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“…In the literature, there are some methods for the production of an amino acid hydrolysate using chrome leather waste. In particular, there is a method for the production of the amino acid fertilizer by hydrolysis of the organic waste using isolated enzymes ( 40 – 42 ). In our current work, we sought to determine whether amino acid residues could have toxic or stimulatory properties.…”

Section: Discussionmentioning

confidence: 99%

Biotransformation of protein-rich waste by Yarrowia lipolytica IPS21 to high-value products—amino acid supernatants

Wieczorek,

Gendaszewska,

Miśkiewicz

et al. 2023

Microbiol Spectr

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The yeast strain Yarrowia lipolytica IPS 21 was tested for its ability to degrade potentially toxic chrome-tanned leather shavings (CTLS) in a liquid environment. Biological and chemical parameters were monitored during a 48-h period of biotransformation of the protein-rich waste. CTLS was added at a concentration of 0.1–4% (wt/wt) to a modified YPG medium (15 g L −1 yeast extract and 5 g L −1 NaCl). Biodegradation and bioconversion were performed in a one-step process. It was found that the higher degradation rate depended on the activity of the proteases and the pH of the medium, but not on the initial inoculum ratio and the activity of the dehydrogenase. The highest efficiency of the process was obtained for 4% (wt/wt) CTLS on day 2 (degradation rate 58–67%, biomass production 2.11–2.20 g L −1 , protease activity 312 U mg −1 protein, and pH 9.20). Our results showed that total chromium was probably not transported across the cytoplasmic membrane of Y. lipolytica IPS21 and that chromium (III) was not oxidized to chromium (VI). The phytotoxicity of selected amino acid supernatants [2.5% (vol/vol)] was tested after the bioconversion process. It was found that the supernatants had a stimulating effect on the plants tested. The root elongation was 29–28% higher than that of the reference samples. This result makes Y. lipolytica IPS21 a potential candidate for safely converting potentially toxic protein-rich wastes into valuable products without enzyme isolation, e.g., amino acid fertilizers. IMPORTANCE Enzyme technologies have the greatest practical relevance to environmental trends. Overcoming the barrier of the high cost of carbon substrates used for biotransformation is the main challenge of these methods. The huge potential of the use of extracellular proteases of Yarrowia species or amino acids in various industries indicates the need for the extension of basic research on waste as a carbon source for this yeast. The experiments demonstrated that it is possible to use Y. lipolytica IPS21 for bioconversion of chrome-tanned leather shavings (CTLS) in a single-step process and to produce high-value amino acid supernatant without having an isolated enzyme. In our study, we show the effect of 2.5% (vol/vol) CTLS supernatant obtained from Y. lipolytica IPS21 on the elongation of the root system of selected plants and provide information on the effect of environmental factors on the efficiency of the bioconversion and the migration of chromium.

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Section: Discussionmentioning

confidence: 99%

Biotransformation of protein-rich waste by Yarrowia lipolytica IPS21 to high-value products—amino acid supernatants

Wieczorek,

Gendaszewska,

Miśkiewicz

et al. 2023

Microbiol Spectr

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“…The molecular weight of the collagen hydrolysate can be influenced by the reaction mixture and concentration of enzymes. Also, the economy of the process can be influenced by a whole series of factors, such as enzyme price, enzyme concentration, reaction speed, reaction time, and reaction yield [ 81 ].…”

Section: Enzymatic Extraction Of Chromiummentioning

confidence: 99%

Depollution of Polymeric Leather Waste by Applying the Most Current Methods of Chromium Extraction

Codreanu (Manea),

Stefan,

Kim

et al. 2024

Polymers

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The leather industry is one of the most polluting industries in the world due to the large amounts of waste following raw hide processing but also due to the high content of chemical substances present in leather waste. The main problem with chromium-tanned leather solid waste is related to the storage, due to the ability of chromium to leach into soil or water, and also owing to the high ability of trivalent chromium to oxidize to its toxic form, hexavalent chromium. The purpose of this article is to present the most current methods of chromium extraction from solid tanned leather waste in order to obtain non-polluting leather, which can constitute secondary raw material in new industrial processes. The extraction methods identified in the present study are based on acid/basic/enzymatic hydrolysis and substitution with the help of organic chelators (organic acids and organic acid salts). In addition, this study includes a comparative analysis of the advantages and disadvantages of each identified extraction method. At the same time, this study also presents alternative chromium extraction methods based on the combination of conventional extraction methods and ultrasound-assisted extraction.

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“…The molecular weight of the collagen hydrolysate can be influenced by the reaction mixture and concentration of enzymes. Also, the economy of the process can be influenced by a whole series of factors, such as: enzyme price, enzyme concentration, reaction speed, reaction time, reaction yield [75].…”

Section: Enzymatic Extraction Of Chromiummentioning

confidence: 99%

The Most Used Methods of Chrome Extraction from Leather Polymer Wastes

Codreanu (Manea),

Stefan,

Kim

et al. 2024

Preprint

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The leather industry is one of the most polluting industries in the world due to the large amounts of waste following the raw hides processing, but also due to the high content of chemicals present in their composition. The main problem of chromium-tanned leather solid waste is related to their storage, because of the ability of chromium to leach in soil or water, and also owing to the high ability of trivalent chromium to oxidize to its toxic form, hexavalent chromium. The purpose of this study is to present the latest methods for extracting trivalent chromium from the composition of solid leather wastes. The extraction methods identified in the present study are based on acid/basic/enzymatic hydrolysis and substitution with the help of organic chelators (organic acids and organic acid salts). In addition, the study includes a comparative analysis between the advantages and disadvantages of each identified extraction method. At the same time, the study also presentes alternative chromium extraction methods based on the combination of conventional extraction methods and ultrasound-assisted extraction.

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Technological-economic optimization of enzymatic hydrolysis used for the processing of chrome-tanned leather waste

Cited by 14 publications

References 31 publications

Biotransformation of protein-rich waste by Yarrowia lipolytica IPS21 to high-value products—amino acid supernatants

Biotransformation of protein-rich waste by Yarrowia lipolytica IPS21 to high-value products—amino acid supernatants

Depollution of Polymeric Leather Waste by Applying the Most Current Methods of Chromium Extraction

The Most Used Methods of Chrome Extraction from Leather Polymer Wastes

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