Technological Advances of 3D Scaffold-Based Stem Cell/Exosome Therapy in Tissues and Organs

Gu, Chenyang; Feng, Jia; Waqas, Ahmed; Deng, Yushu; Zhang, Yifan; Chen, Wanghao; Long, Jun; Huang, Shiying; Chen, Lukui

doi:10.3389/fcell.2021.709204

Cited by 26 publications

(14 citation statements)

References 172 publications

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“…It also provides a suitable matrix environment for cell adhesion and growth [ 46 ] and maintains cellular functions, such as cytoskeleton reorganization, integrin activation, gene expression, and ECM organization [ 47 ]. Compared with cell suspensions, cell-laden scaffolds show higher cell viability and better integration at the host site [ 48 ]. Furthermore, in tissues where cell orientations are necessary, a good alignment of cells and ECM is feasible.…”

Section: Corneal Regenerative Approach: Cell-based Vs Scaffold-based ...mentioning

confidence: 99%

Human SMILE-Derived Stromal Lenticule Scaffold for Regenerative Therapy: Review and Perspectives

Santra

Liu

Jhanji

et al. 2022

IJMS

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A transparent cornea is paramount for vision. Corneal opacity is one of the leading causes of blindness. Although conventional corneal transplantation has been successful in recovering patients’ vision, the outcomes are challenged by a global lack of donor tissue availability. Bioengineered corneal tissues are gaining momentum as a new source for corneal wound healing and scar management. Extracellular matrix (ECM)-scaffold-based engineering offers a new perspective on corneal regenerative medicine. Ultrathin stromal laminar tissues obtained from lenticule-based refractive correction procedures, such as SMall Incision Lenticule Extraction (SMILE), are an accessible and novel source of collagen-rich ECM scaffolds with high mechanical strength, biocompatibility, and transparency. After customization (including decellularization), these lenticules can serve as an acellular scaffold niche to repopulate cells, including stromal keratocytes and stem cells, with functional phenotypes. The intrastromal transplantation of these cell/tissue composites can regenerate native-like corneal stromal tissue and restore corneal transparency. This review highlights the current status of ECM-scaffold-based engineering with cells, along with the development of drug and growth factor delivery systems, and elucidates the potential uses of stromal lenticule scaffolds in regenerative therapeutics.

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Section: Corneal Regenerative Approach: Cell-based Vs Scaffold-based ...mentioning

confidence: 99%

Human SMILE-Derived Stromal Lenticule Scaffold for Regenerative Therapy: Review and Perspectives

Santra

Liu

Jhanji

et al. 2022

IJMS

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“…The effectiveness of 3D scaffold-based exosome treatment for skin regeneration has been examined in several research. Wang et al verified that a biocompatible 3D porous self-healing methylcellulose-chitosan hydrogel, supplied with placental MSC-derived exosomes, promoted wound healing by cooperatively promoting angiogenesis and inhibiting apoptosis [ 106 ]. Therefore, using secretome 3D printing technology for wound healing is a promising area for further research.…”

Section: Structural Formulation Using Biomaterials With Secretome For...mentioning

confidence: 99%

Cell Secretome Strategies for Controlled Drug Delivery and Wound-Healing Applications

et al. 2022

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There is significant interest in using stem cells in the management of cutaneous wounds. However, potential safety, efficacy, and cost problems associated with whole-cell transplantation hinder their clinical application. Secretome, a collective of mesenchymal stem-cell-stored paracrine factors, and immunomodulatory cytokines offer therapeutic potential as a cell-free therapy for the treatment of cutaneous wounds. This review explores the possibility of secretome as a treatment for cutaneous wounds and tissue regeneration. The review mainly focuses on in vitro and in vivo investigations that use biomaterials and secretome together to treat wounds, extend secretome retention, and control release to preserve their biological function. The approaches employed for the fabrication of biomaterials with condition media or extracellular vesicles are discussed to identify their future clinical application in wound treatment.

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“…From the perspective of pathogenesis, 85% of strokes are ischemic strokes, which are caused by intracranial vascular occlusion, leading to apoptosis or necrosis of cells in the brain, and changes in neurovascular units and brain damage. Secondary cell death after ischemic stroke is coordinately mediated by multiple pathophysiological mechanisms, such as inflammation, oxidative stress, vascular dysfunction, energy depletion, excitotoxicity, autophagy, and apoptosis ( 4 , 5 ).…”

Section: Introducationmentioning

confidence: 99%

The PI3K/AKT Pathway—The Potential Key Mechanisms of Traditional Chinese Medicine for Stroke

Zhang

et al. 2022

Front. Med.

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Stroke is associated with a high disability and fatality rate, and adversely affects the quality of life of patients and their families. Traditional Chinese Medicine (TCM) has been used effectively in the treatment of stroke for more than 2000 years in China and surrounding countries and regions, and over the years, this field has gleaned extensive clinical treatment experience. The Phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) pathway is important for regulation of cell migration, proliferation, differentiation, and apoptosis, and plays a vital role in vascularization and oxidative stress in stroke. Current Western medicine treatment protocols for stroke include mainly pharmacologic or mechanical thrombectomy to restore blood flow. This review collates recent advances in the past 5 years in the TCM treatment of stroke involving the PI3K/AKT pathway. TCM treatment significantly reduces neuronal damage, inhibits cell apoptosis, and delays progression of stroke via various PI3K/AKT-mediated downstream pathways. In the future, TCM can provide new perspectives and directions for exploring the key factors, and effective activators or inhibitors that affect occurrence and progression of stroke, thereby facilitating treatment.

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Technological Advances of 3D Scaffold-Based Stem Cell/Exosome Therapy in Tissues and Organs

Abstract: Graphical AbstractA summary schematic of application of 3D scaffold-based stem cell/exosome therapy.

Cited by 26 publications

References 172 publications

Human SMILE-Derived Stromal Lenticule Scaffold for Regenerative Therapy: Review and Perspectives

Human SMILE-Derived Stromal Lenticule Scaffold for Regenerative Therapy: Review and Perspectives

Cell Secretome Strategies for Controlled Drug Delivery and Wound-Healing Applications

The PI3K/AKT Pathway—The Potential Key Mechanisms of Traditional Chinese Medicine for Stroke

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