Technological advances in hydrogen production by Enterobacter bacteria upon substrate, luminosity and anaerobic conditions

Souza, Isadora Torres de; Moreira, Felipe Santos; Ferreira, Juliana de Souza; Cardoso, Vicelma Luiz; Batista, Fabiana Regina Xavier

doi:10.1016/j.ijhydene.2019.05.036

Cited by 9 publications

(3 citation statements)

References 38 publications

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“…Other factors that also contribute to antagonist action of LAB include the production of hydrogen peroxide, bacteriocins, and antibiotic-like substances (Arqués et al, 2015). Other minor organic acids produced during the fermentative process can be associated with the growth of sub-dominant bacteria reported by the 16S rRNA gene high-throughput sequencing, including succinic acid produced by Leuconostoc or Acinetobacter, propionic acid from hexose metabolism of Enterobacter species, and acetic acid by both acetic acid bacteria and heterofermentative LAB (Andriani et al, 2019;Souza et al, 2019;Kang et al, 2012).…”

Section: Substrates and Metabolitesmentioning

confidence: 99%

Presence and persistence of Pseudomonas sp. during Caspian Sea-style spontaneous milk fermentation highlights the importance of safety and regulatory concerns for traditional and ethnic foods

et al. 2021

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The aim of this study was to evaluate the performance of Caspian Sea-style spontaneous milk fermentation to improve the quality of pasteurized milk containing high levels of Pseudomonas contamination, with a focus on microbiological safety and stability of the final product. Bacterial diversity of pasteurized milk, fermentation process, and after 60 days of storage was analyzed by Illumina-based sequencing, and presence of viable taxa was confirmed by culturing on selective media. Low quality pasteurized milk harbored mainly Gram-negative bacteria, markedly dominated by Pseudomonas. Following fermentation, lactic acid bacteria rapidly became dominant with maximum population of 10.15 log CFU/mL at 18 h, represented mainly by Lactococcus. However, sequences related to Pseudomonas, and to a lesser extent for enterobacteria, remained constant throughout the fermentation process. The cultured-dependent approach confirmed the presence of viable Pseudomonas, with a final population of 5.60 log CFU/mL. Biochemical transformations were further analyzed, indicating lactic acid as the main end-metabolite produced (maximum concentration of 5.93 g/L at 24 h). In addition, the increase of 2-nonanone can be correlated as a volatile biomarker of P. aeruginosa and related species. Altogether, the results demonstrated that natural milk fermentation may often not inhibit the development of pathogens and food spoilage microorganisms.

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Section: Substrates and Metabolitesmentioning

confidence: 99%

Presence and persistence of Pseudomonas sp. during Caspian Sea-style spontaneous milk fermentation highlights the importance of safety and regulatory concerns for traditional and ethnic foods

et al. 2021

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“…are believed to be the most efficient hydrogen producer, some studies outline that Enterobacter sp. can also yield a high hydrogen production [58][59][60], on some occasions even higher than Clostridium sp. [61].…”

Section: Effect Of the Inoculum Type On The Metabolic Profilesmentioning

confidence: 99%

“…An oxygen-free environment can be achieved by nitrogen sparging or by microorganisms that deplete oxygen (facultative anaerobes). However, previous studies have stressed that nitrogen sparging not only increases the hydrogen production costs, but also dilutes the hydrogen content in the acidogenic off-gas [59,63], hence, the importance of facultative anaerobes in DF is highlighted.…”

Section: Effect Of the Inoculum Type On The Metabolic Profilesmentioning

confidence: 99%

Dark Fermentation Process Response to the Use of Undiluted Tequila Vinasse without Nutrient Supplementation

et al. 2021

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The technical feasibility of valorizing tequila vinasse (TV), a wastewater with high pollution potential, through the production of biogenic hydrogen via dark fermentation, has long been proven in diverse lab-scale reactors that were operated either in batch or continuous mode. However, such systems have mainly been tested with diluted streams and nutrient supplementation, hindering the techno-economic attractiveness of the TV-to-hydrogen concept at large scale. In this study, the feasibility of producing hydrogen from high-strength undiluted TV with no added extra nutrients was evaluated under batch mesophilic conditions. Additionally, the use of two different acidogenic inocula obtained either by heat or heat-aeration pretreatment was investigated to get a greater understanding of the effect of inoculum type on the process. The results obtained showed that the TV utilized herein contained macro- and micro-nutrients high enough to support the hydrogenogenic activity of both cultures, entailing average hydrogen yields of 2.4–2.6 NL H2/L vinasse and maximum hydrogen production rates of 1.4–1.9 NL H2/L-d. Interestingly, the consumption of lactate and acetate with the concomitant production of butyrate was observed as the main hydrogen-producing route regardless of the inoculum, pointing out the relevance of the lactate-driven dark fermentative process. Clostridium beijerinckii was ascertained as key bacteria, but only in association with microorganisms belonging to the genera Enterobacter and Klebsiella, as revealed by phylogenetic analyses.

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Overview of glycerol as a carbon source in the hydrogen production process

Pio,

Silva,

Cardoso

et al. 2023

Biomass Conv. Bioref.

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Technological advances in hydrogen production by Enterobacter bacteria upon substrate, luminosity and anaerobic conditions

Cited by 9 publications

References 38 publications

Presence and persistence of Pseudomonas sp. during Caspian Sea-style spontaneous milk fermentation highlights the importance of safety and regulatory concerns for traditional and ethnic foods

Presence and persistence of Pseudomonas sp. during Caspian Sea-style spontaneous milk fermentation highlights the importance of safety and regulatory concerns for traditional and ethnic foods

Dark Fermentation Process Response to the Use of Undiluted Tequila Vinasse without Nutrient Supplementation

Overview of glycerol as a carbon source in the hydrogen production process

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