Technological advances in bovine mastitis diagnosis

Duarte, Carla; Freitas, P. P.; Bexiga, Ricardo

doi:10.1177/1040638715603087

Cited by 70 publications

(71 citation statements)

References 78 publications

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“…Evidence‐based mastitis therapy implies the identification of the MCP before antibiotic treatment is initiated (DVG, ). Conventional bacteriological culture and PCR are the most common and reliable tools for diagnosis of MCP but do not allow for timely treatment decisions (Duarte, Freitas, & Bexiga, ). The time span between milk sampling and the arrival of the microbiological and sensitivity test result amounts to at least 48–72 hr including approximately 24 hr of transportation time to the laboratory (Mansion‐de Vries et al., ; Neeser, Hueston, Godden, & Bey, ; Owens, Ray, Watts, & Yancey, ).…”

Section: Mastitis Management—therapeutic Measuresmentioning

confidence: 99%

Mastitis treatment—Reduction in antibiotic usage in dairy cows

Krömker¹,

Leimbach²

2017

Reprod Domestic Animals

184

134

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Contents Animal‐friendly, economical, resource‐saving milk production provides the basis for sustained consumer acceptance. Bovine mastitis plays a decisive role in the dairy industry—disturbing animal health and welfare and causing considerable economic losses on the other hand. Currently, antimicrobial treatment is indispensable to keep bovine udder health, animal welfare and economic aspects in balance. On the contrary, emergence and spread of antimicrobial resistance (AMR) is an urgent matter of particular public interest, and as a consequence, antimicrobial usage (AMU) in production livestock is a critically discussed subject. In urgent need of future reduction in AMU in the dairy industry, this review article describes and discusses possible approaches promising prompt implementation, including therapeutical alternatives as well as pro‐ and metaphylactic concepts such as the implementation of evidence‐based mastitis therapy concepts and selective dry cow treatment (sDCT), in search of the most effective and contemporary methods for decreasing AMU and AMR in dairy production.

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Section: Mastitis Management—therapeutic Measuresmentioning

confidence: 99%

Mastitis treatment—Reduction in antibiotic usage in dairy cows

Krömker¹,

Leimbach²

2017

Reprod Domestic Animals

184

134

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“…Mastitis is capable of affecting the mammary glands and it changes milk composition (Korhonen & Kaartinen, 1995). Ot is a disease with high treatment costs which makes mastitis an economical concern for dairy farmers (Duarte et al, 2015). S. aureus is one of the main pathogens causing mastitis in dairy animals, which leads to overuse of antimicrobial agents (Roberson et al, 1998;Peles et al, 2007;Dlde Riekerink et al, 2006;Barkema et al, 2009;Gomes & Henriques, 2016).…”

Section: Discussionmentioning

confidence: 99%

Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk

et al. 2020

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Staphylococcus aureus is the leading cause of intoxications in humans. Also, methicillin-resistant S. aureus (MRSA) is an emerging problem in food-producing animals. The presence of MRSA in milk may create a risk to public health. The aim of this study was to evaluate the presence of MRSA in bulk tank milk samples. Dne hundred and twenty bulk tank milk samples were analyzed using the convenient sampling method. The isolates were confirmed by real-time polymerase chain reaction (RT-PCR) targeting nuc and mecA gene in S. aureus. Antibiotic resistance profiles of the isolates were tested by disc agar diffusion method. On the current study, S. aureus was detected in 44 (36.66%) of the samples. The presence of the mecA gene was found to be positive in 40 (75.4%) of the 53 S. aureus isolates. As a result of the study, a high incidence of MRSA was detected in bulk tank milk samples. Antibiotic-resistant bacteria are at risk of being transferred to humans via milk. For safe and healthy milk consumption, uncontrolled use of antibiotics in dairy cows should be avoided.

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“…An inherent weakness in routine methods seems to be that the expression of characteristics varies by Strep. uberis isolate (Odierno et al, 2006;Krömker et al, 2014;Duarte et al, 2015). The routine identification procedure could be misleading with respect to the distinction between atypical Strep.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic accuracy of a standardized scheme for identification of Streptococcus uberis in quarter milk samples: A comparison between conventional bacteriological examination, modified Rambach agar medium culturing, and 16S rRNA gene sequencing

Wald

Baumgartner

Urbantke

et al. 2017

Journal of Dairy Science

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Bacteriological examination of milk samples is a prerequisite for pathogen-specific therapy and aids in limiting antimicrobial resistance. The aims of this study were to establish a standardized scheme for reliable Streptococcus uberis identification in routine diagnosis and to evaluate the accuracy of conventional tests and growing patterns of Strep. uberis on a selective medium (modified Rambach agar medium, MRAM) using 16S rRNA gene sequencing analysis as a reference method. We obtained isolates of presumptive Strep. uberis (n = 336) from quarter milk samples of dairy cows with intramammary infections and classified the isolates into 2 clusters using biochemical characterization. In cluster 1 (n = 280), cocci grew as non-hemolytic colonies, hydrolyzing esculin, carrying no Lancefield antigen (A/B/C/D/G) or Christie Atkins Munch-Petersen factor, and their growth was inhibited on an Enterococcus agar. Production of β-d-galactosidase on MRAM was shown by 257 of the cluster 1 isolates (91.79%), and 16S rRNA gene sequencing verified 271 (96.79%) of the isolates to be Strep. uberis. In 264 isolates (94.29%), MRAM agreed with the sequencing results. In cluster 2 (n = 56), isolates showed different characteristics: 37 (66.07%) were β-d-galactosidase-positive, and based on 16S sequencing results, 36 (64.29%) were identified correctly as Strep. uberis using biochemical methods. Identification success in this group differed significantly between routine diagnosis and MRAM application: MRAM agreed with sequencing results in 47 isolates (83.93%). To identify Strep. uberis and differentiate it from other lactic acid bacteria in routine diagnosis, we suggest using catalase reaction, hemolysis, esculin hydrolysis, and growth on enterococci agar. Isolates that show a typical biochemical profile can be identified satisfactorily with these tests. For Strep. uberis isolates with divergent patterns, application of MRAM as a follow-up test increased the diagnostic accuracy to 94.64%.

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Technological advances in bovine mastitis diagnosis

Cited by 70 publications

References 78 publications

Mastitis treatment—Reduction in antibiotic usage in dairy cows

Mastitis treatment—Reduction in antibiotic usage in dairy cows

Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk

Diagnostic accuracy of a standardized scheme for identification of Streptococcus uberis in quarter milk samples: A comparison between conventional bacteriological examination, modified Rambach agar medium culturing, and 16S rRNA gene sequencing

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