Technoeconomic evaluation of bio-based styrene production by engineered <i>Escherichia coli</i>

Claypool, Joshua T.; Raman, D. Raj; Jarboe, Laura R.; Nielsen, David R.

doi:10.1007/s10295-014-1469-5

Cited by 33 publications

(20 citation statements)

References 18 publications

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“…Another historically inexpensive petroleum-derived bulk chemical is styrene (1.9$/kg). 51 The demand for styrene shows a steady increase from 2.0 million metric tons (Mt) in 1970 to 3.2 million Mt in 1980 and 5.8 million Mt in 2004 in the United States alone. 234 A number of aromatic building blocks on Figure 23 could be potentially selectively defunctionalized to styrene; however, this will be the subject of future research.…”

Section: Catalytic Strategies Aiming At High Yield and Selective Prodmentioning

confidence: 99%

Bright Side of Lignin Depolymerization: Toward New Platform Chemicals

et al. 2018

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Lignin, a major component of lignocellulose, is the largest source of aromatic building blocks on the planet and harbors great potential to serve as starting material for the production of biobased products. Despite the initial challenges associated with the robust and irregular structure of lignin, the valorization of this intriguing aromatic biopolymer has come a long way: recently, many creative strategies emerged that deliver defined products via catalytic or biocatalytic depolymerization in good yields. The purpose of this review is to provide insight into these novel approaches and the potential application of such emerging new structures for the synthesis of biobased polymers or pharmacologically active molecules. Existing strategies for functionalization or defunctionalization of lignin-based compounds are also summarized. Following the whole value chain from raw lignocellulose through depolymerization to application whenever possible, specific lignin-based compounds emerge that could be in the future considered as potential lignin-derived platform chemicals.

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Section: Catalytic Strategies Aiming At High Yield and Selective Prodmentioning

confidence: 99%

Bright Side of Lignin Depolymerization: Toward New Platform Chemicals

et al. 2018

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“…Currently, styrene production predominantly comes from the energy-intensive chemocatalytic dehydrogenation of petroleum-derived ethylbenzene [ 2 , 3 ]. Because of concerns over depleting feedstock availability and deleterious environmental impacts, a bio-based method could be a low energy, renewable alternative to petroleum-derived styrene [ 4 ]. Thus, an artificial pathway for styrene and hydroxystyrene biosynthesis from glucose in Escherichia coli was previously engineered [ 5 – 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, several of artificial biosynthetic pathways have been engineered in microorganisms to produce useful, functionalized phenolic compounds from glucose [ 4 , 6 , 11 – 15 ]. In our laboratory, we investigate artificial biosynthetic pathways in microorganisms to produce a number of useful phenylpropanoids from plants [ 16 – 18 ].…”

Section: Introductionmentioning

confidence: 99%

Artificial de novo biosynthesis of hydroxystyrene derivatives in a tyrosine overproducing Escherichia coli strain

et al. 2015

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BackgroundStyrene and its derivatives as monomers and petroleum-based feedstocks are valuable as raw materials in industrial processes. The chemical reaction for styrene production uses harsh reaction conditions such as high temperatures or pressures, or requires base catalysis with microwave heating. On the other hand, production of styrene and its derivatives in Escherichia coli is an environmental friendly process to produce conventional petroleum-based feedstocks.ResultsAn artificial biosynthetic pathway was developed in E. coli that yields 4-hydroxystyrene, 3,4-dihydroxystyrene and 4-hydroxy-3-methoxystyrene from simple carbon sources. This artificial biosynthetic pathway has a codon-optimized phenolic acid decarboxylase (pad) gene from Bacillus and some of the phenolic acid biosynthetic genes. E. coli strains with the tal and pad genes, the tal, sam5, and pad genes, and the tal, sam5, com, and pad genes produced 4-hydroxystyrene, 3,4-dihydroxystyrene and 4-hydorxy-3-methoxystyrene, respectively. Furthermore, these pathways were expressed in a tyrosine overproducing E. coli. The yields for 4-hydroxystyrene, 3,4-dihydroxystyrene and 4-hydorxy-3-methoxystyrene reached 355, 63, and 64 mg/L, respectively, in shaking flasks after 36 h of cultivation.ConclusionsOur system is the first to use E. coli with artificial biosynthetic pathways for the de novo synthesis of 3,4-dihydroxystyrene and 4-hydroxy-3-methoxystyrene in a simple glucose medium. Similar approaches using microbial synthesis from simple sugar could be useful in the synthesis of plant-based aromatic chemicals.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-015-0268-7) contains supplementary material, which is available to authorized users.

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“…53 As a note, for this study, styrene is not considered to be a renewably sourceable monomer; however, styrene can be produced biologically and this is an active area of research. [54][55][56] In the present work, methacrylic acid and acrylic acid were used as reactive diluents to enable the use of the rPET-polymer backbones in the FRPs and to improve material properties. Methacrylic acid can be produced via decarboxylation of itaconic acid, 57,58 which can be obtained biologically in high yields.…”

Section: Discussionmentioning

confidence: 99%

Combining Reclaimed PET with Bio-based Monomers Enables Plastics Upcycling

Rorrer

Nicholson

Carpenter

et al. 2019

Joule

208

167

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This study develops an approach to incentivize both higher extents of waste plastics reclamation and use of bio-based chemicals. In particular, reclaimed plastics (polyethylene terephthalate) and chemicals derivable from renewable resources are combined to create high-performance, long-lifetime composite materials with properties that exceed those of standard petroleum-based materials and that exhibit higher selling prices than reclaimed plastic. Analysis predicts that this approach results in reductions in energy input and greenhouse gas emissions relative to standard composites manufacturing today.

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Technoeconomic evaluation of bio-based styrene production by engineered Escherichia coli

Cited by 33 publications

References 18 publications

Bright Side of Lignin Depolymerization: Toward New Platform Chemicals

Bright Side of Lignin Depolymerization: Toward New Platform Chemicals

Artificial de novo biosynthesis of hydroxystyrene derivatives in a tyrosine overproducing Escherichia coli strain

Combining Reclaimed PET with Bio-based Monomers Enables Plastics Upcycling

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