Techno-functional, biological and structural properties of Spirulina platensis peptides from different proteases

Akbarbaglu, Zahra; Ayaseh, Ali; Ghanbarzadeh, Babak; Sarabandi, Khashayar

doi:10.1016/j.algal.2022.102755

Cited by 27 publications

(38 citation statements)

References 48 publications

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“…This can be due to different proteolytic actions of enzymes applied in this study. Our results were in line with those by Akbarbaglu et al ( 2022 ) who reported that hydrolysate samples obtained from Spirulina platensis protein by the action of pepsin and pancreatin had higher contents of functional amino acids (EAA, AAA, and charged AA). Due to chemical structure and functional groups of these amino acids, they are capable of scavenging free radicals and can act as an effective antioxidant in biological cells.…”

Section: Resultssupporting

confidence: 93%

“…The amide III band (1210–1320 cm −1 ) is attributed to alpha‐helix, beta‐sheet, beta‐turn, and random coils structures (Akbarbaglu et al, 2022 ). The intensity of this band in H‐Pep and H‐Pan samples was lower than that of obtained for primary CPP.…”

Section: Resultsmentioning

confidence: 99%

“…These amino acids convert free radicals to their stable forms by proton or electron donation (You et al, 2009 ). Hydrolysates obtained from the action of pepsin and pancreatin in other sources of proteins, for example, mung bean (Xie et al, 2019 ), walnuts (Moghadam et al, 2020 ), and Spirulina platensis (Akbarbaglu et al, 2022 ; Mohammadi et al, 2022 ) showed enhanced antioxidant activity too.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, pancreatin, trypsin, and pepsin enzymes were used for enzymatic hydrolysis of corn pollen protein. The conditions of the hydrolysis process were selected based on the optimal activity point of each enzyme previously tested and optimized in our laboratory (Akbarbaglu et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

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Physicochemical, antioxidant, antimicrobial, and in vitro cytotoxic activities of corn pollen protein hydrolysates obtained by different peptidases

Akbarmehr

Peighambardoust

Ghanbarzadeh

et al. 2023

Food Science & Nutrition

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The applications of protein hydrolysates as food preservatives and nutraceutical ingredients have attracted much attention because of their beneficial effects. The interest in these ingredients has shifted toward their biological activities with benefits to human health. Bioactive peptides are known as antioxidant agents that could promote health‐promoting effects and prolong food shelf‐life beyond their basic nutritional value. Thus, the aim of this study was to investigate antioxidant, antimicrobial, and in vitro cytotoxic properties of corn pollen protein (CPP) hydrolysates obtained by different enzymes. Proteolytic activity in terms of degree of hydrolysis (DH) and SDS‐PAGE analysis was measured in pancreatin (H‐Pan), pepsin (H‐Pep), and trypsin (H‐Tri) hydrolysates. Amino acid composition, antioxidant and antimicrobial activities, and cytotoxicity of hydrolysates were evaluated. DH and SDS‐PAGE revealed higher proteolytic activity of pepsin compared to other enzymes. Amino acid analysis showed that the functional amino acids such as antioxidant types were most predominant in H‐Pep compared to two other samples. Antioxidant activity of hydrolysates was found to be affected by the type of enzyme and the concentration of hydrolysates. There was a significant difference ( p < 0.05) between antioxidant activity of different hydrolysates. The highest antioxidant activity in terms of Trolox equivalent antioxidant capacity (0.23–2.75 mM), DPPH (33.3%–64.8%), and hydroxyl (33.7%–63.2%) radical scavenging activities, chelation of iron (33.2%–62.5%) and copper (30.2%–50.5%) metals, and total antioxidant activity (0.65–0.85) was obtained for H‐Pep followed by H‐Pan and H‐Tri samples. Antibacterial tests showed that pepsin‐hydrolyzed protein was not significantly ( P > 0.05) effective against E. coli at any concentrations, however, it showed significant ( P < 0.05) concentration‐dependent effect against S. aureus (with inhibition zones of 15–25 mm). Cytotoxicity results revealed that CPP, as a nonhydrolyzed protein, did not generally show antiproliferative activity, however, a significant ( P < 0.05) ability of H‐Pep hydrolysate in decreasing HT‐29 colon cancer cell line viability was seen in a concentration‐dependent manner (the lowest cell viability of 32% at 5 mg/mL). Overall, investigating the application of protein‐based hydrolysates is one of the possible strategies that govern their applied intentions as preservatives and nutraceuticals in the food and pharmaceutical industries.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Physicochemical, antioxidant, antimicrobial, and in vitro cytotoxic activities of corn pollen protein hydrolysates obtained by different peptidases

Akbarmehr

Peighambardoust

Ghanbarzadeh

et al. 2023

Food Science & Nutrition

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“…So far, the exact reason for such condition remains unknown. In term of S. platensis, flavonoid compound of S. platensis was actually expected to function as a bacterial clearing agent (Akbarbaglu et al, 2022). In accordance with this, S. platensis administration resulted in reduced caecal LNE content in this study, although the values did not reach the significant levels.…”

Section: Discussionsupporting

confidence: 81%

Dietary supplementation of Spirulina platensis and Saccharomyces cerevisiae on egg quality, physiological condition and ammonia emission of hens at the late laying period

Wahyuni

Yudiarti

Widiastuti

et al. 2023

J. Indonesian Trop. Anim. Agric.

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The study was aimed to evaluate the effect of Spirulina platensis and Saccharomyces cerevisiae or its combination on egg quality, physiological condition and ammonia excretion of hens at the late lay-ing period. At 81 weeks old, 144 Lohmann Brown layer chickens were divided into four treatment groups included CON (hens fed basal feed), SP (basal feed + 0.3% S. platensis), SC (basal feed + 0.2% S. cerevisiae), and SPSC (basal feed + 0.3% S. platensis + 0.2% S. cerevisiae). At the end of the study, eggs, intestinal mucosa, digesta, excreta and blood sample were collected. Results showed that albumin index was higher (P<0.05) in SPSC group than in CON, SP and SC. The yolk index and yolk colour were greater (P<0.05) in SP and SPSC groups than in CON and SC. The erythrocyte values were high-er (P<0.05) in SP group compared to CON, SC and SPSC groups. The ileum pH was higher (P<0.05) in SP than in CON, SC and SPSC. Lactic acid bacteria counts were lower (P<0.05) in the caecum of SC and SPSC than in CON and SP groups. The lower (P<0.05) counts of lactose-negative Enterobacte-riaceae were shown in SC and SPSC than in CON. The excreta pH was lower (P<0.05) in SC group compared to CON, SP and SPSC groups. There were better (P<0.05) protein digestibility coefficient and nitrogen retention in SPSC group than others. Faecal ammonia decreased (P<0.05) in SP, SC and SPSC groups. In conclusion, S. platensis improved egg yolk index and colour, increased erythrocyte counts and played an important role in maintaining the balance of bacteria in the intestine resulting in reduced ammonia excretion. Dietary inclusion of S. cerevisiae reduced ammonia excretion of laying hens during the late laying period.

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Physicochemical characteristics and antioxidant stability of spray‐dried soy peptide fractions

Akbarbaglu

Tamjidi

Sarabandi

et al. 2023

Food Science & Nutrition

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Nowadays, with increasing human population and industrialization, and changing lifestyle, there are some concerns regarding to poor quality of foods and prevalence of chronic diseases. Therefore, scientists and manufacturers are trying to produce functional foods by fortifying food products with natural bioactive compounds. Some of the most common health-promoting substances include polyphenols, carotenoids, anthocyanins, vitamins, minerals, probiotics, fatty acids, phytosterols, and bioactive peptides.Proteins are inexpensive, economic, available, and rich sources of bioactive peptides. Protein-derived bioactive peptides are specific encrypted and inactive fragments within the parent protein, which may exert various health-enhancing functions after release by different methods, such as enzymolysis (Udenigwe, 2014). Some of their beneficial physiological effects include anti-aging, anti-cancer,

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Techno-functional, biological and structural properties of Spirulina platensis peptides from different proteases

Cited by 27 publications

References 48 publications

Physicochemical, antioxidant, antimicrobial, and in vitro cytotoxic activities of corn pollen protein hydrolysates obtained by different peptidases

Physicochemical, antioxidant, antimicrobial, and in vitro cytotoxic activities of corn pollen protein hydrolysates obtained by different peptidases

Dietary supplementation of Spirulina platensis and Saccharomyces cerevisiae on egg quality, physiological condition and ammonia emission of hens at the late laying period

Physicochemical characteristics and antioxidant stability of spray‐dried soy peptide fractions

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