Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria

Piddock, L. J. V.

doi:10.1111/j.1365-2672.1990.tb02880.x

Cited by 122 publications

(70 citation statements)

References 34 publications

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“…Furthermore, the inhibition zone sizes for the 32 T. maritimum strains grown on MA with ENR and OTC were significantly lower (P Ͻ 0.05) than those on the other four media tested. This result is perhaps not surprising due to the qualitative and quantitative differences in the composition of the MA in comparison with those of the oligotrophic media, mainly in the carbon and nitrogen sources, and the presence of excessive amounts of divalent cations, which are known to affect the susceptibility testing results obtained with tetracycline in other culture media, such as tryptone yeast extract salts agar (25,26).…”

Section: Resultsmentioning

confidence: 85%

Recommendation of an Appropriate Medium for In Vitro Drug Susceptibility Testing of the Fish Pathogen Tenacibaculum maritimum

Avendaño‐Herrera

Irgang

Núñez

et al. 2005

Antimicrob Agents Chemother

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In the present study, Anacker and Ordal agar, marine agar (MA), and Flexibacter maritimus medium (FMM) were compared with the dilute versions of Mueller-Hinton agar (DMHA) medium recommended by the National Committee for Clinical Laboratory Standards (NCCLS) for their use in disk diffusion tests with Tenacibaculum maritimum strains and to calculate the MICs of five drugs by the Etest method. Preliminary growth tests performed with 32 strains of this pathogen on each medium revealed that all strains failed to grow on DMHA, while the remaining media supported good growth of all isolates. In the susceptibility tests, which were carried out with the other three media, all strains were resistant to oxolinic acid and were highly susceptible to amoxicillin and trimethoprim-sulfamethoxazole, showing a good correspondence with the Etest values, which ranged from 0.064 to 0.75 and 0.006 to 1.5 g/ml, respectively. Enrofloxacin and oxytetracycline produced significantly smaller inhibition zones and MICs on MA than on the other media assayed. However, fast, clear, and well-defined zones of inhibition were displayed for all strains at 24 h of incubation only on FMM by both the disk diffusion assay and Etest. In addition, FMM prepared with commercial sea salts instead of seawater was also suitable for bacterial isolation as well as for susceptibility testing. On the basis of these results, the use of FMM to determine the in vitro susceptibility of T. maritimum and its inclusion in a future revision of the NCCLS M42 report are recommended.

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Section: Resultsmentioning

confidence: 85%

Recommendation of an Appropriate Medium for In Vitro Drug Susceptibility Testing of the Fish Pathogen Tenacibaculum maritimum

Avendaño‐Herrera

Irgang

Núñez

et al. 2005

Antimicrob Agents Chemother

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“…Mycobacteria isolates were also tested for their antibiotic susceptibility according to Piddock [11] by using 10 antibiotic disks: rifampin, kanamycin, streptomycin, azithromycin, ciprofloxacin, tobramycin, cephalothin, pyrazinamide, isoniazid, ethambutol.…”

Section: Methodsmentioning

confidence: 99%

Isolation and identification of Mycobacterium avium complex and other nontuberculosis mycobacteria from drinking-water in Basra governorate, Iraq

Al-Sulami

Al-Taee²,

Wida’a³

2012

East Mediterr Health J

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“…The tubes incubated for determination of MIC in the liquid medium were used to determine the MBC (Phillips, 1991;Piddock, 1990;Clsi, 2007). An aliquot (1ml) of each of it was inoculated into Mueller Hinton Agar plates, and subsequently these plates were incubated at room at 37 ° C for 24h.…”

Section: Minimum Bactericidal Concentrations (Mbc) Of the Ee And Solumentioning

confidence: 99%

“…The MIC determination was performed using the macrodilution method (Phillips, 1991;Piddock, 1990;Nccls, 2003;Clsi, 2007). For this test each bacterial suspension was homogenized in BHI broth in a ratio of 1: 1000 (v / v), which was obtained from a bacterial concentration of about 1-2 x 105 CFU / mL.…”

Section: Minimum Inhibitory Concentrations (Mic) Of the Ee And Solutionmentioning

confidence: 99%

Assessment in Vitro of Antibacterial Activity of Manipulated Product, on Solution Form, Obtained from Dry Extract of Leaves of Psidium guajava L.

Silva

Frazão²,

Ferreira³

et al. 2015

IJC

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The Psidium guajava L. specie is a perennial shrub, belonging to the Myrtaceae family and it is popularly known as guava, its leaves are used in therapy for treating various diseases. The study aims to evaluate the antibacterial activity in vitro of manipulated product obtained from dried extract of the leaves of P. guajava L. front standard bacteria ATCC and clinical isolates. The tests were conducted on bacterial samples: Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27883), Escherichia coli (ATCC 25922), Salmonella spp, Acinetobacter baumannii, Proteus mirabilis, Shigella flexneri, Staphylococcus epidermidis, Staphylococcus haemolyticus, Streptococcus agalactiae, Streptococcus mutans. Among the tests can be mentioned phytochemical of the P. guajava leaves ethanolic extract (EE), microbiological control and physical-chemical analysis of the product and microbiological tests such as agar diffusion method (wells), minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), an evaluation test of hemolytic capacity of the solution and in vitro assay cytotoxic activity were performed, . The best result of the product in the agar diffusion method was front Staphylococcus epidermidis, while the lower MIC and MBC were front Staphylococcus aureus (ATCC 25923). The product showed no hemolytic activity and no cytotoxic activity at the tested concentrations. According to the test results, it is believed on the possibility of the production of a pharmaceutical formulation derived from the dry extract of Psidium guajava, since it showed great antibacterial activity.

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Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria

Cited by 122 publications

References 34 publications

Recommendation of an Appropriate Medium for In Vitro Drug Susceptibility Testing of the Fish Pathogen Tenacibaculum maritimum

Recommendation of an Appropriate Medium for In Vitro Drug Susceptibility Testing of the Fish Pathogen Tenacibaculum maritimum

Isolation and identification of Mycobacterium avium complex and other nontuberculosis mycobacteria from drinking-water in Basra governorate, Iraq

Assessment in Vitro of Antibacterial Activity of Manipulated Product, on Solution Form, Obtained from Dry Extract of Leaves of Psidium guajava L.

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