Techniques of Immunohistochemistry: Principles, Pitfalls and Standardization

Taylor, Clive R.; Shi, Shan‐Rong; Barr, Nancy J.; Wu, Nancy

doi:10.1016/b978-0-443-06652-8.50007-7

Cited by 81 publications

(127 citation statements)

References 116 publications

Supporting

Mentioning

118

Contrasting

Unclassified

Order By: Relevance

“…Immunohistochemistry determination of VEGF protein. [14][15][16][17][18] was detected by using a rabbit anti-human polyclonal anti-VEGF antibody. Sections boiled for 10 minutes in 10mmol/sodium citrate buffer for antigen retrieval followed by quenching of endogenous peroxidase activity, and then blocking with 10% goat serum.…”

Section: Methodsmentioning

confidence: 99%

Correlation of endothelial cell proliferation with vascular endothelial growth factor in endometrium of women with menorrhagia

2017

View full text Add to dashboard Cite

Background: Approximately 30% of women of reproductive age experience excessive blood loss during menstruation. In 50% of cases, menorrhagia has no underlying pathology. However, until recently, the only permanent cure for menorrhagia was hysterectomy. In this study we aim to determine the correlation of vascular endothelial growth factor (VEGF) expression with markers of endometrial endothelial cell proliferation like proliferating cell nuclear antigen (PCNA) and Cluster Determination (CD34).Methods: A total of 100 patients with history of menorrhagia were selected for study. Double Immunohistochemistry was performed on these endometrial biopsy sections. Proliferating endothelial cells were identified by an immunohistochemical double staining technique with PCNA and CD34. VEGF expression was also seen in endometrial biopsy.Results: In general, expression of both VEGF and PCNA was more in functional layer than basal layer in both menorrhagic patients as well as non menorrhagic patients. When glandular cytoplasmic VEGF expression was compared with PCNA the association was statistically significant whereas completely opposite findings was seen with glandular luminal surface VEGF positivity but the association was statistically significant. In secretory phase (p-value<0.001) there was highly statistically significant association in PCNA grading with glandular luminal surface VEGF positivity whereas when we correlated PCNA with cytoplasmic glandular VEGF in secretory phase it was statistically significant (p-value<0.001).Conclusions: The endothelial proliferation was significantly higher in menorrhagia patients during late secretory phase of cycle than controls. We were able to demonstrate increased endothelial proliferation in patients in the premenstrual part of cycle.

show abstract

Section: Methodsmentioning

confidence: 99%

Correlation of endothelial cell proliferation with vascular endothelial growth factor in endometrium of women with menorrhagia

2017

View full text Add to dashboard Cite

show abstract

“…A primary panel of antibodies consisting Cytokeratin (ck), Leucocyte common antigen (LCA), Vimentin, S100 was applied and incubated followed by secondary antibody and peroxidase antiperoxidase complex. 3-3' diaminobezidine tetrahydochloride (DAB) was used as a chromogen then counterstained by hematoxylin and mounted in distrene dibutylphthalate (DPX) [10][11] . Table 4] Among 26 cases of hematopoietic lineage, 16 cases (61.53%) were diagnosed as Non Hodgkin's Lymphoma (DLBCL), all were positive for LCA and CD20.…”

Section: Methodsmentioning

confidence: 99%

Role of Immunohistochemistry in Diagnosis of Undifferentiated Malignant Tumours

Dhakar¹

2018

jmscr

View full text Add to dashboard Cite

“…Immunostaining modules for SOX-2, MAP2, and GFAP were used according to protocols and instructions provided by the supplier (Ventana Medical System, Inc.). All immunostaining protocols were based on a streptavidin-biotin complex (20)(21)(22)(23) .…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemical analysis of retinoblastoma cell phenotype using neuronal and glial cell markers

Orellana¹,

Neto²,

Antecka³

et al. 2016

Arquivos Brasileiros De Oftalmologia

View full text Add to dashboard Cite

Purpose: The cellular origin of retinoblastoma is uncertain as constituent tumor cells heterogeneously express markers of both immature and mature retinal cells. An immunohistochemical analysis of cellular origin may yield valuable insights into disease progression and treatment options. This study aimed to determine the cellular origin of retinoblastoma in a large case series and correlate these findings with histopathological prognostic factors. Methods: Thirty-nine retinoblastoma cases were histopathologically diagnosed and analyzed by immunohistochemistry using monoclonal antibodies against the immature neural cell marker SRY-box containing gene 2 (SOX-2), the mature neuronal cell marker microtubule-associated protein 2 (MAP2), and the mature glial cell marker glial fibrillary acidic protein (GFAP). Histopathological features were also evaluated, including patterns of growth, differentiation, vitreous see ding, and choroidal/scleral, optic nerve, and anterior chamber invasion. Two retinoblastoma cell lines, WERI-1 and Y79, were studied by immunocytochemistry using the same antibodies. Results: Expression of SOX-2 was strong in 97.4% of retinoblastoma cases, while MAP-2 was expressed in 59% of cases. Immunostaining for GFAP was positive only in reactive stromal astrocytes interspersed amongst tumor cells and in peritumoral tissue. There was no correlation between histopathological prognostic factors and immunohistochemical markers. Retinoblastoma cell lines showed strong positivity for SOX2 (90% of WERI-1 cells and 70% of Y79 cells) and MAP2 (90% of cells in both lines). GFAP was completely negative in both cell lines. Conclusion: The majority of retinoblastomas and both RB cell lines expressed an immature neural and/or a mature neuronal cell marker, but not a glial marker. These results indicate a typical neuroblast or neuronal origin and eliminate astrocyte differentiation from neural stem cells as the source of retinoblastoma.Keywords: Retinoblastoma/etiology; Retinoblastoma/pathology; Phenotype; Prognosis; Immunohistochemistry; Antibodies, monoclonal RESUMO Objetivos: Este estudo visa determinar a origem do retinoblastoma em um número de casos e correlacionar essos achados com fatores prognósticos e histopatológicos conhecidos. Métodos: Trinta e nove casos de retinoblastoma foram diagnosticados e analisados com imuno-histoquímica usando marcadores de anticorpos monoclonais contra as células de retina imaturas (SOX-2: SRY-box containing gene 2), contra as células da retina maturas (MAP2: microtubule -associated protein 2) e contra as células gliais maturas (GFAP: glial fibrillar acidic protein). Foram avaliadas características microscópicas dos casos (grau de diferenciação, presença de semeadura vítrea, invasão de coroide/esclera, nervo óptico e câmara anterior). Duas linhas celulares de retinoblastoma (WERI-1 e Y79) também foram testadas, utilizando os três marcadores. Resultados: A expressão de SOX-2 foi positiva em 97,4% dos casos de retinoblastoma, enquanto MAP2 foi positivo em 59% dos casos. ...

show abstract

Techniques of Immunohistochemistry: Principles, Pitfalls and Standardization

Cited by 81 publications

References 116 publications

Correlation of endothelial cell proliferation with vascular endothelial growth factor in endometrium of women with menorrhagia

Correlation of endothelial cell proliferation with vascular endothelial growth factor in endometrium of women with menorrhagia

Role of Immunohistochemistry in Diagnosis of Undifferentiated Malignant Tumours

Immunohistochemical analysis of retinoblastoma cell phenotype using neuronal and glial cell markers

Contact Info

Product

Resources

About