Techniques microbiolization seed forage radish with Trichoderma spp. and Bacillus subtilis

Abstract

The objective of this study was the effect of leverage microbiolization forage radish seed with Trichoderma spp. and Bacillus subtilis by physiological conditioning techniques, suspension of biological structures and film coating. The microbiolization suspension of biological structures was carried out with commercial products Rhizoliptus ® and Agrotrich plus ® . Fluid restriction was held in PDA medium + mannitol (-0.7 MPa), when was the first seed radicle protrusion in the other were removed and dried in a laboratory environment for 48 h. The film coating was performed with the addition of the polymer Color Seed ® (300 mL kg -1 ) treatment of the syrup containing Trichoderma spp. or product Rhizoliptus ® . We used a treatment covering the seeds primed with organisms, or their association. The microbiolization with Trichoderma spp. by physiological conditioning controls pathogens infecting the seeds. Trichoderma spp. and Bacillus subtilis promote the growth and development of seedlings of forage radish, and the coating of the seeds primed in the presence of organisms which provides greater shoot growth of seedlings in the field. Técnicas de microbiolização de sementes de nabo forrageiro com Trichoderma spp. e Bacillus subtilis RESUMO O objetivo deste trabalho foi potencializar o efeito da microbiolização de sementes de nabo forrageiro com Trichoderma spp. e Bacillus subtilis pelas técnicas de condicionamento fisiológico, suspensão de estruturas biológicas e peliculização. A microbiolização com suspensão de estruturas biológicas foi realizada com os produtos comerciais Agrotrich plus ® e Rhizoliptus ® . A restrição hídrica foi realizada em meio BDA + Manitol (-0,7 MPa), ao ocorrer protrusão radicular na primeira semente, as demais foram retiradas e secas em ambiente de laboratório por 48 h. A peliculização foi realizada com a adição do polímero Color Seed ® (300 mL kg -1 ) à calda de tratamento contendo Trichoderma spp. ou ao produto Rhizoliptus. Foi utilizado um tratamento recobrindo as sementes condicionadas com os organismos isolados ou em associação. A microbiolização com Trichoderma spp. pelo condicionamento fisiológico controla os patógenos infectantes das sementes. Trichoderma spp. e Bacillus subtilis promovem o crescimento e desenvolvimento de plântulas de nabo forrageiro, sendo o recobrimento das sementes condicionadas na presença dos organismos o que proporciona maior crescimento de parte aérea de plântulas no campo.

Biopriming in black oat seeds

“…Hölbig et al (2011) observed that the use of films in trials with coating of hydroconditioned onion seeds impaired seedling vigor. Junges et al (2017) observed that the coating of forage turnip seeds, which had been osmotically conditioned in the presence of the same organisms, provided greater seedling shoot growth in the field. The root growth of black oat seedlings was only reduced with water restriction in isolation for the fungus, not representing a reduction in root dry matter, which did not differ between treatments.…”