Techniques for measuring intracellular Ca2+ concentrations in the isolated perfused heart

Bentivegna, L

doi:10.1016/0022-2828(91)91381-z

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The investigation of these pathways and the related mechanisms regulating calcium hemostasis have been aided by quantitative techniques that allow for the direct measurement of [Ca2+)1 accumulation. [13][14][15] Nuclear magnetic resonance (NMR), bioluminescent, and fluorescent indicators have all been used to advantage to measure [Ca2+)1 accumulation during ischemia in the isolated perfused heart. NMR studies have shown that in the ischemic rat heart increased cytosolic calcium accumulation and ATP depletion were linked and may directly contribute to lethal ischemic injury.13,1s The use of NMR provides a sensitive measurement of cytosolic calcium accumulation in the ischemic myocardium but does not allow the recording of beat-to-beat cytosolic calcium changes.…”

Section: Cvtosolic Calcium Determinationmentioning

confidence: 99%

Myocardial Cytosolic Calcium Accumulation During Ischemia/Reperfusion: The Effects of Aging and Cardioplegia

McCully

Tsukube

Ataka

et al. 1994

Journal of Cardiac Surgery

View full text Add to dashboard Cite

Cytosollc calcium In the myocardium Is rapidly accumulated during Ischemia and has been correlated with the attenuation of functional recovery In the myocardium. The aged myocardium Is more sensitive to Ischemia and accumulates significantly more cytosollc cal· clum than either the newborn or the mature myocardium. Modification of the age-related pro· penalty to Increased cytosollc calcium accumulation may be achieved through the use of magnesium or potassium/magnesium cardioplegia. Improved postlschemlc ventricular func· tlon obtained with magnesium or potassium/magnesium cardioplegia may have Important lm· pllcatlons In the reduction of myocardial morbidity and mortality. (J Card Surg 1994; 9[Supp/]:449-452)

show abstract

Section: Cvtosolic Calcium Determinationmentioning

confidence: 99%