Techniques for improving the specificity of sandwich enzyme‐linked immunosorbent assay‐based drug screening
Rohan Steel,
Antony Botteon,
Mark Timms
Abstract:ELISA assays are commonly used for drug screening by racing laboratories but are known to suffer from limited specificity. Inaccurate ELISA screening results are typically produced by non‐specific antibody interactions or by the retention of chromogenic material in the sample well due to sample degradation. While confirmation of drug positives can be achieved by mass spectrometry, the follow‐up of inaccurate ELISA screening results represents an unnecessary cost in staff time and reagents. This is particularly… Show more
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