by a 1.6-mm mesh screen (Fowler et al. 1983) and returned to germinating conditions until their roots were 1-2 cm long. They were then placed in hydroponic tanks filled with continuously aerated one-half strength modified Hoagland's solution.Temperatures were maintained at 25"C day and night with a 16 h day/8 h night for one day, then reduced to 22"C day/15'C night untii the plants reached the threeleaf stage (approximately 7 d). Daylength was then reduced to 72h and the following temperature regime was followed: 5'C day/3"C night for 7 d; 3"C dayi I "C night for 14 d; 2"C dayl -2"C night for 14 d.
LTro DeterminationPlants were prepared and frozen by a method similar to that described by Fowler and Carles (1979)