Techniques for analyzing biomaterial surface structure, morphology and topography

Murthy, N. Sanjeeva

doi:10.1533/9780857090768.2.232

Cited by 12 publications

(4 citation statements)

References 25 publications

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“…In addition to the surface chemistry, the surface wettability is influenced by the topography (Figure ). , AFM analysis showed that the mean roughness ( R a ) increased as PS < poly(DTEc) < gold < poly(NaSS) < Ti6Al4V (Figure ). Thus, smooth, hydrophobic surfaces are obtained by spin coating of PS and poly(DTEc).…”

Section: Resultsmentioning

confidence: 96%

Competitive Adsorption of Plasma Proteins Using a Quartz Crystal Microbalance

Felgueiras

Murthy

Sommerfeld

et al. 2016

ACS Appl. Mater. Interfaces

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Proteins that get adsorbed onto the surfaces of biomaterials immediately upon their implantation mediate the interactions between the material and the environment. This process, in which proteins in a complex mixture compete for adsorption sites on the surface, is determined by the physicochemical interactions at the interface. Competitive adsorption of bovine serum albumin (BSA), fibronectin (Fn), and collagen type I (Col I), sequentially and from mixtures, was investigated so as to understand the performances of different surfaces used in biomedical applications. A quartz crystal microbalance with dissipation was used to monitor the adsorption of these proteins onto two materials used in functional bone replacement, a titanium alloy (Ti6Al4V) and Ti6Al4V physisorbed with poly(sodium styrenesulfonate) [poly(NaSS)], and three controls, gold, poly(desaminotyrosyltyrosine ethyl ester carbonate) [poly(DTEc)], and polystyrene (PS). In experiments with individual proteins, the adsorption was the highest with Fn and Col I and the least with BSA. Also, protein adsorption was the highest on poly(NaSS) and Ti6Al4V and the least on poly(DTEc). In sequential adsorption experiments, protein exchange was observed in BSA + Fn, Fn + Col I, and BSA + Col I sequences but not in Fn + BSA and Col I + BSA because of the lower affinity of BSA to surfaces relative to Fn and Col I. Protein adsorption was the highest with Col I + Fn on hydrophobic surfaces. In experiments with protein mixtures, with BSA & Fn, Fn appears to be preferentially adsorbed; with Fn & Col I, both proteins were adsorbed, probably as multilayers; and with Col I & BSA, the total amount of protein was the highest, greater than that in sequential and individual adsorption of the two proteins, probably because of the formation of BSA and Col I complexes. Protein conformational changes induced by the adsorbing surfaces, protein-protein interactions, and affinities of proteins appear to be the important factors that govern competitive adsorption. The findings reported here will be useful in understanding the host response to surfaces used for implants.

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Section: Resultsmentioning

confidence: 96%

Competitive Adsorption of Plasma Proteins Using a Quartz Crystal Microbalance

Felgueiras

Murthy

Sommerfeld

et al. 2016

ACS Appl. Mater. Interfaces

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“…Digital photos of the inner surfaces of seed cotyledon were taken before and after each CAP treatment using the Keyence digital microscope VHX-600 with ×5,000 optical lens (Murthy 2011). For depth profiling, the image stacks were collected under identical conditions (i.e., similar area and vertical resolution) at 5 μm Z intervals.…”

Section: Microscopy and Image Analysismentioning

confidence: 99%

Inactivation of Surface-Borne Microorganisms and Increased Germination of Seed Specimen by Cold Atmospheric Plasma

Mitra

Klämpfl

et al. 2013

Food Bioprocess Technol

166

117

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Sustaining the quality of seeds is a major task in attempting to supply nutrition to the growing world population. In this study, the seeds of Cicer arietinum were exposed to cold atmospheric plasma (CAP). A significant reduction of the natural microbiota attached to the seed surface was observed for increasing CAP treatment times-2 and 5 min were sufficient to achieve a 1 and 2 log reductions, respectively. Furthermore a 1 min CAP treatment showed a strongly improved seed germination (89.2 %), speed of germination (7.1±0.1 seeds/day), and increased seed vigor, beside a decrease in the mean germination time (2.7 days) compared with controls. The roughness profile of the seed cotyledon was altered significantly, only in case of longer treatment times from 5 min. These results suggest that CAP technology has the potentiality to reduce health risks associated with contaminated seeds, while improving food quality.

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“…CLSM is a common technique used to examine the surface properties of materials using either light emission or absorption techniques [24]. Herein, a fluorescein dye probe [25,26] was used to map the surface of the pellets.…”

Section: Confocal Laser Scanning Microscopy (Clsm)mentioning

confidence: 99%

Animal Biopolymer-Plant Biomass Composites: Synergism and Improved Sorption Efficiency

2020

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Pelletized biomaterial composites that contain chitosan (C) and torrefied wheat straw (S) at variable weight composition (C:S) were prepared using a facile blending process. The fractional content of the wheat straw was studied to elucidate the role of biomass on the pelletized product and effects of S-content on the physicochemical properties relevant to adsorption phenomena. Chitosan pellets (with and without S) were characterized by spectroscopic (FT-IR and 13C NMR) and thermal (TGA and DSC) techniques to provide support for their respective C:S composition. Confocal microscopy using fluorescein (FL) as a dye probe revealed the presence and an increase in the accessibility of the active sites for the composite pellets according to the S-content (wt %). Equilibrium and kinetic sorption studies using FL and reactive black (RB) dyes revealed an incremental adsorption affinity of the pellets with anionic dyes in variable charge states (FL and RB). The trend for dye adsorption parallels the incremental S-content (wt %) in the composite pellets. This study reports a first-example of a low-cost, facile, and sustainable approach for the valorization of straw and chitosan suitable for sorption-based applications in aqueous media.

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Techniques for analyzing biomaterial surface structure, morphology and topography

Cited by 12 publications

References 25 publications

Competitive Adsorption of Plasma Proteins Using a Quartz Crystal Microbalance

Competitive Adsorption of Plasma Proteins Using a Quartz Crystal Microbalance

Inactivation of Surface-Borne Microorganisms and Increased Germination of Seed Specimen by Cold Atmospheric Plasma

Animal Biopolymer-Plant Biomass Composites: Synergism and Improved Sorption Efficiency

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