Techniques and applications in supercritical fluid chromatography

Greibrokk, Tyge; Berg, B. E.; Blilie, Ann Lisbeth.; Doehl, Jo; Farbrot, Anne; Lundanes, Elsa

doi:10.1016/s0021-9673(01)83810-3

Cited by 26 publications

(11 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This means that the partition coefficient must increase, because the other terms in this case are constant. Substituting Equation 3 into 6 , 4 into 7, and 5 into 8, By simplifying Equation 9 step by step, we obtain Equation 12. and temperatures are determined experimentally. The temperatures do not have to be exactly the temperatures that will be used for solute focusing.…”

Section: Partition Coefflclent Focusingmentioning

confidence: 99%

Solute focusing in supercritical fluid chromatography

Koski

Lee

1991

J Microcolumn Separations

View full text Add to dashboard Cite

Abstract. Solute focusing is an important topic in chromatography, but it has not been discussed extensively in the recent supercritical fluid chromatography literature. The purpose of this paper is to review the different methods that have been used to focus solutes in supercritical fluid chromatography. The theoretical basis for phase ratio and partition coefficient focusing is described. Different solute focusing devices that are used in on-line supercritical fluid extraction and chromatography are shown. The methods and principles of removing the solvent from the solutes prior to the analytical column, and hence reducing band broadening in the column due to the solvent, are discussed in context with the use of a retention gap or a mixing chamber, and in solvent vent injection.

show abstract

Section: Partition Coefflclent Focusingmentioning

confidence: 99%

Solute focusing in supercritical fluid chromatography

Koski

Lee

1991

J Microcolumn Separations

View full text Add to dashboard Cite

show abstract

“…Focusing was accomplished only by means of the temperature gradient. Other groups (8,13,14) have also reported the use of temperature to vary the mobile phase density for improved focusing of the sample at the front of the analytical column.…”

Section: Solute Focusingmentioning

confidence: 99%

“…TO increase the effectiveness and the speed of solvent elimination, a separate precolumn could be connected to the analytical column via the vent valve as was reported by Greibrokk et al (8,13) (Figure 6). In this way, the resistance of the analytical column to the purging gas, as well as some potentially negative effects of the solvent vapor on the stationary phase film (such as swelling), can be eliminated.…”

Section: Solvent Vent Techniquesmentioning

confidence: 99%

Liquid sample introduction methods in capillary column supercritical fluid chromatography

Lee

Huang

et al. 1989

J Microcolumn Separations

View full text Add to dashboard Cite

“…The main advantages of SFC are the versatility during both Separation and detection [4,5,6]. Separations can be optimized by proper choice of supercritical fluid -with or Without modifier-and the stationary phase, and by density programming achieved via pressure or temperature regulation.…”

Section: Introductionmentioning

confidence: 99%

Packed-capillary supercritical fluid chromatography of pesticides using phosphorus-selective detection

et al. 1991

View full text Add to dashboard Cite

SummaryPacked-capillary, supercritical fluid chromatography using carbon dioxide modified with methanol or 2-propanol as mobile phase, was coupled with a thermionic detector for Phosphorus-selective detection. Optimum conditions for thermionic detection were established for a number of hydrogen flow rates by adjusting the air flow rate and bead Position. Using the mentioned modifiers, a series of organOphosphorus pesticides of varying polarity could be separated within 8 min. An optimum sensitivity of 55-128 fg P/ s and linearity over four ordcrs of magnitude were obtained. The detector showed good selectivity for phosphorus Over carbon and nitrogen, viz., 9 9 104 g C/g P and 75 g N/ g P, respectively. This was demonstrated by determining pesticides such as phoxim, dimethoatc and azinphos-methyl In onion and tomato extracts.

show abstract

Techniques and applications in supercritical fluid chromatography

Cited by 26 publications

References 24 publications

Solute focusing in supercritical fluid chromatography

Solute focusing in supercritical fluid chromatography

Liquid sample introduction methods in capillary column supercritical fluid chromatography

Packed-capillary supercritical fluid chromatography of pesticides using phosphorus-selective detection

Contact Info

Product

Resources

About